Faisal F Alotaibi, Zainab M AlFaltawi, Sharon R Oyhanart, Jonathan C Knowles, Francesco D'Aiuto, David Y S Chau
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Membranes were then analyzed using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), contact angle measurements (CAM), and cell culture-based assays.</p><p><strong>Results: </strong>FTIR revealed similar collagen spectra among membranes. SEM showed structural similarities between ESMs and Bio-Gide. DSC indicated integrity maintenance at 37°C and varied storage conditions. CAM testing demonstrated collagen-based membranes' higher hydrophilicity compared to d-PTFE. DMA analysis showed duck ESM's superior tensile strength and Young's modulus compared to chicken ESM and porcine CM. Biological evaluation revealed high compatibility with human gingival fibroblasts for all materials.</p><p><strong>Conclusion: </strong>Findings illustrate a novel sustainable biomaterial that could be utilized for GBR and other periodontal therapies, particularly in its capacity to function as a physical barrier consistent with the traditional role of GBR membranes.</p>","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":" ","pages":"285-298"},"PeriodicalIF":2.6000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355667/pdf/","citationCount":"0","resultStr":"{\"title\":\"The eggshell membrane as a barrier membrane for guided bone regeneration.\",\"authors\":\"Faisal F Alotaibi, Zainab M AlFaltawi, Sharon R Oyhanart, Jonathan C Knowles, Francesco D'Aiuto, David Y S Chau\",\"doi\":\"10.1080/17460751.2025.2542056\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The eggshell membrane (ESM) is a natural resource with a distinct design and composition, offering structural features consistent with barrier membranes used in guided bone regeneration (GBR), making it a promising candidate for this application. 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DMA analysis showed duck ESM's superior tensile strength and Young's modulus compared to chicken ESM and porcine CM. 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引用次数: 0
摘要
背景：蛋壳膜（ESM）是一种具有独特设计和组成的自然资源，其结构特征与用于引导骨再生（GBR）的屏障膜一致，使其成为该应用的有前途的候选者。本研究旨在通过将鸡和鸭esm与可吸收性猪源胶原蛋白（Porcine CM, Bio-Gide®）和不可吸收性聚四氟乙烯（d-PTFE, CytoplastTM）进行比较，评估其作为GBR替代品的可行性。方法：采用标准化提取方法提取esm。然后使用扫描电子显微镜（SEM），傅里叶变换红外光谱（FTIR），差示扫描量热法（DSC），动态力学分析（DMA），接触角测量（CAM）和基于细胞培养的分析对膜进行分析。结果：FTIR显示膜间胶原蛋白谱相似。SEM显示esm与Bio-Gide结构相似。DSC显示在37°C和不同的储存条件下保持完整性。CAM测试表明，与d-PTFE相比，胶原基膜具有更高的亲水性。DMA分析表明，鸭ESM的抗拉强度和杨氏模量优于鸡ESM和猪CM。生物学评价表明，所有材料均与人牙龈成纤维细胞具有良好的相容性。结论：研究结果说明了一种新的可持续生物材料可用于GBR和其他牙周治疗，特别是其作为物理屏障的功能与GBR膜的传统作用一致。
The eggshell membrane as a barrier membrane for guided bone regeneration.
Background: The eggshell membrane (ESM) is a natural resource with a distinct design and composition, offering structural features consistent with barrier membranes used in guided bone regeneration (GBR), making it a promising candidate for this application. This study aims to assess the feasibility of chicken and duck ESMs as GBR alternatives by comparing them with resorbable porcine-derived collagen (Porcine CM, Bio-Gide®) and non-resorbable dense polytetrafluoroethylene (d-PTFE, CytoplastTM).
Methods: ESMs were extracted using a standardized protocol. Membranes were then analyzed using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), contact angle measurements (CAM), and cell culture-based assays.
Results: FTIR revealed similar collagen spectra among membranes. SEM showed structural similarities between ESMs and Bio-Gide. DSC indicated integrity maintenance at 37°C and varied storage conditions. CAM testing demonstrated collagen-based membranes' higher hydrophilicity compared to d-PTFE. DMA analysis showed duck ESM's superior tensile strength and Young's modulus compared to chicken ESM and porcine CM. Biological evaluation revealed high compatibility with human gingival fibroblasts for all materials.
Conclusion: Findings illustrate a novel sustainable biomaterial that could be utilized for GBR and other periodontal therapies, particularly in its capacity to function as a physical barrier consistent with the traditional role of GBR membranes.
期刊介绍：
Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization.
Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community.
Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.
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