从塑料薄膜温室土壤中分离出的灰顶双歧杆菌及其重新分类为梳状灰顶双歧杆菌的建议。11月。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-08-09 DOI:10.1007/s00284-025-04430-8

Xinyu Liu, Hailong Liang, Yingying Liu, Huijun Zhang, Hui Han, Bingyue Xin, Xiaomin Wu, Feng Li, Man Song, Long Zhang

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引用次数: 0

摘要

从淮北地膜温室土壤中分离到一株革兰氏阴性、可动、棒状、淡黄色菌株LB2ST，并采用多相法对其分类位置进行了研究。生长条件为15-45℃（最适30℃）、pH 5.0-9.0（最适pH 8.0）和0-1.5% (w/v) NaCl（最适0% NaCl）。菌株LB2ST的16S rRNA基因序列与propanilivorax Oryzisolibacter EPL6T相似度最高（97.4%），其次是嗜碱三聚氰胺菌（Melaminivora CY1T）（97.1%）、反硝化aliicycliphilus K601T（97.0%）和paenidovorax caeni R-24608 T（96.9%）。菌株LB2ST与其他共藓科植物的配对平均核苷酸同源性（ANI）、平均氨基酸同源性（AAI）和数字DNA-DNA杂交（dDDH）值分别低于85.3%、85.0%和29.4%。综合系统发育、表型和基因型数据，菌株LB2ST （= CCTCC AB 2023230 T = KCTC 8325 T）代表了Alicycliphilus属的一个新种，建议将其命名为Alicycliphilus soli sp. nov。此外，基于系统基因组分析，建议将肥厚Diaphorobacter limosus重新分类为Alicycliphilus limosus comb。11月。

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Alicycliphilus soli sp. nov., Isolated from a Plastic Film Greenhouse Soil and the Proposal for the Reclassification of Diaphorobacter limosus as Alicycliphilus limosus comb. nov.

A Gram-stain-negative, aerobic, motile, rod-shaped, pale yellow bacterial strain LB2S^T was isolated from a plastic film greenhouse soil in Huaibei, China, and its taxonomic position was investigated using polyphasic approach. Growth occurred at 15-45 ℃ (optimum, 30 °C), at pH 5.0-9.0 (optimum, pH 8.0) and at 0-1.5% (w/v) NaCl (optimum, 0% NaCl). The 16S rRNA gene sequence of strain LB2S^T showed high similarity to Oryzisolibacter propanilivorax EPL6T (97.4% similarity), followed by Melaminivora alkalimesophila CY1T (97.1%), Alicycliphilus denitrificans K601T (97.0%), and Paenacidovorax caeni R-24608^T (96.9%). The pairwise average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridizations (dDDH) values between strain LB2S^T and other Comamonadaceae species were below 85.3%, 85.0% and 29.4%, respectively. Considering the results from phylogeny, phenotypic and genotypic data, strain LB2S^T (= CCTCC AB 2023230^T = KCTC 8325^T) represent a novel species of the genus Alicycliphilus, for which the name Alicycliphilus soli sp. nov. is proposed. Furthermore, based on the phylogenomic analysis, it is recommended that Diaphorobacter limosus be reclassified as Alicycliphilus limosus comb. nov.

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.