Wei Li, Si-Xue He, Yi-Wen Liu, Qian-Yu Zhou, Shu-Fen Xiao, Yanshan Chen, Yue Cao, Lena Q Ma
{"title":"亚砷酸盐反转运蛋白ACR3/3;1对硒增强植物生长和砷超富集植物Pteris multifida和Pteris cretica的砷积累至关重要。","authors":"Wei Li, Si-Xue He, Yi-Wen Liu, Qian-Yu Zhou, Shu-Fen Xiao, Yanshan Chen, Yue Cao, Lena Q Ma","doi":"10.1016/j.jhazmat.2025.139434","DOIUrl":null,"url":null,"abstract":"<p><p>Selenium (Se) enhances plant growth and As accumulation in As-hyperaccumulator Pteris vittata, but its effects on As-hyperaccumulators Pteris multifida and Pteris cretica are unknown. Here, they were exposed to 50 μM arsenate (As<sub>50</sub>) plus 1.25 (Se<sub>1.25</sub>) or 2.5 μM (Se<sub>2.5</sub>) selenate under hydroponics. After 2 weeks of growth, the biomass, As, Se and malondialdehyde (MDA) contents, and important genes related to As metabolism in two plants were determined. Both plants were able to take up and translocate As to the fronds, with Se-enhanced plant growth being observed only under As exposure. For both plants, the As+Se treatments increased their biomass by 22-32 % compared to the As<sub>50</sub> treatment, which may be attributed to 42-45 % reduction in the MDA contents. However, the effects of Se on plant growth and As accumulation varied with Se doses and plant species. For P. multifida, 1.25 µM Se enhanced the As content by 52 % to 329 mg kg<sup>-1</sup> in the fronds, while 2.5 µM Se decreased. For P. cretica, Se promoted its frond As contents by 42-106 % to 155-225 mg kg<sup>-1</sup>, with 2.5 μM being more effective. Though showing little effect on phosphate transporter Pht1 or arsenate reductase HAC1, Se induced upregulation of arsenite antiporters ACR3/3;1 by 1.6-3.0 fold, which promoted As translocation from the roots to fronds and As sequestration into the fronds under As exposure. Se effectively enhances plant growth and As accumulation in P. multifida and P. cretica by upregulating the expression of arsenite antiporters ACR3. Based on literature and our data, ACR3/3;1 play critical roles in Se-enhanced As accumulation in As-hyperaccumulators, which may have application in phytoremediation of As-contaminated soils in temperate zones.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"496 ","pages":"139434"},"PeriodicalIF":11.3000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Arsenite antiporters ACR3/3;1 are critical for Se-enhanced plant growth and As accumulation in As-hyperaccumulators Pteris multifida and Pteris cretica.\",\"authors\":\"Wei Li, Si-Xue He, Yi-Wen Liu, Qian-Yu Zhou, Shu-Fen Xiao, Yanshan Chen, Yue Cao, Lena Q Ma\",\"doi\":\"10.1016/j.jhazmat.2025.139434\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Selenium (Se) enhances plant growth and As accumulation in As-hyperaccumulator Pteris vittata, but its effects on As-hyperaccumulators Pteris multifida and Pteris cretica are unknown. Here, they were exposed to 50 μM arsenate (As<sub>50</sub>) plus 1.25 (Se<sub>1.25</sub>) or 2.5 μM (Se<sub>2.5</sub>) selenate under hydroponics. After 2 weeks of growth, the biomass, As, Se and malondialdehyde (MDA) contents, and important genes related to As metabolism in two plants were determined. Both plants were able to take up and translocate As to the fronds, with Se-enhanced plant growth being observed only under As exposure. For both plants, the As+Se treatments increased their biomass by 22-32 % compared to the As<sub>50</sub> treatment, which may be attributed to 42-45 % reduction in the MDA contents. However, the effects of Se on plant growth and As accumulation varied with Se doses and plant species. For P. multifida, 1.25 µM Se enhanced the As content by 52 % to 329 mg kg<sup>-1</sup> in the fronds, while 2.5 µM Se decreased. For P. cretica, Se promoted its frond As contents by 42-106 % to 155-225 mg kg<sup>-1</sup>, with 2.5 μM being more effective. Though showing little effect on phosphate transporter Pht1 or arsenate reductase HAC1, Se induced upregulation of arsenite antiporters ACR3/3;1 by 1.6-3.0 fold, which promoted As translocation from the roots to fronds and As sequestration into the fronds under As exposure. Se effectively enhances plant growth and As accumulation in P. multifida and P. cretica by upregulating the expression of arsenite antiporters ACR3. Based on literature and our data, ACR3/3;1 play critical roles in Se-enhanced As accumulation in As-hyperaccumulators, which may have application in phytoremediation of As-contaminated soils in temperate zones.</p>\",\"PeriodicalId\":94082,\"journal\":{\"name\":\"Journal of hazardous materials\",\"volume\":\"496 \",\"pages\":\"139434\"},\"PeriodicalIF\":11.3000,\"publicationDate\":\"2025-09-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of hazardous materials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jhazmat.2025.139434\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/6 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of hazardous materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.jhazmat.2025.139434","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/6 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Arsenite antiporters ACR3/3;1 are critical for Se-enhanced plant growth and As accumulation in As-hyperaccumulators Pteris multifida and Pteris cretica.
Selenium (Se) enhances plant growth and As accumulation in As-hyperaccumulator Pteris vittata, but its effects on As-hyperaccumulators Pteris multifida and Pteris cretica are unknown. Here, they were exposed to 50 μM arsenate (As50) plus 1.25 (Se1.25) or 2.5 μM (Se2.5) selenate under hydroponics. After 2 weeks of growth, the biomass, As, Se and malondialdehyde (MDA) contents, and important genes related to As metabolism in two plants were determined. Both plants were able to take up and translocate As to the fronds, with Se-enhanced plant growth being observed only under As exposure. For both plants, the As+Se treatments increased their biomass by 22-32 % compared to the As50 treatment, which may be attributed to 42-45 % reduction in the MDA contents. However, the effects of Se on plant growth and As accumulation varied with Se doses and plant species. For P. multifida, 1.25 µM Se enhanced the As content by 52 % to 329 mg kg-1 in the fronds, while 2.5 µM Se decreased. For P. cretica, Se promoted its frond As contents by 42-106 % to 155-225 mg kg-1, with 2.5 μM being more effective. Though showing little effect on phosphate transporter Pht1 or arsenate reductase HAC1, Se induced upregulation of arsenite antiporters ACR3/3;1 by 1.6-3.0 fold, which promoted As translocation from the roots to fronds and As sequestration into the fronds under As exposure. Se effectively enhances plant growth and As accumulation in P. multifida and P. cretica by upregulating the expression of arsenite antiporters ACR3. Based on literature and our data, ACR3/3;1 play critical roles in Se-enhanced As accumulation in As-hyperaccumulators, which may have application in phytoremediation of As-contaminated soils in temperate zones.