集成微流控管的3d打印光遗传神经探针,用于视蛋白/药物输送。

IF 3.9 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Revathi Sukesan, Mohsin Mohammed, Keonghwan Oh, Malvika Sharma, Dipesh Chaudhury, Sohmyung Ha
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引用次数: 0

摘要

光遗传学以其精确的神经刺激而闻名,是行为研究不可或缺的一部分,可以研究涉及决策,记忆,社会互动和运动的神经回路。传统的方法需要两次单独的手术:第一次是将含有视蛋白基因的病毒载体传递到目标大脑区域,第二次是植入光探针进行光刺激。这种双步骤过程增加了组织损伤和注射和植入部位错位的风险。在这项研究中,我们提出了一种3d打印的多模态光遗传神经探针,它将光传递和流体注入结合在一个设备中。通过将市售的微流控管与3d打印光探针集成在一起,该设备为各种应用提供了快速和可定制的组件。该探针被植入小鼠丘脑底核,使病毒载体的传递和装置的植入在一个单一的程序。在病毒表达之后,行为实验表明,光刺激增加了传播距离和速度,证实了有效的神经元激活。免疫组织化学分析显示,通过mCherry标记神经元,通道视紫红质-2 (ChR2(H134R))成功表达,减少了植入部位周围星形细胞(GFAP)和小胶质细胞(ED1)的激活,并保留了NeuN染色证实的神经元群。这些结果突出了该设备的生物相容性、最小的炎症反应和长期神经调节的适用性,在研究和临床环境中具有潜在的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

3D-printed optogenetic neural probe integrated with microfluidic tube for opsin/drug delivery.

3D-printed optogenetic neural probe integrated with microfluidic tube for opsin/drug delivery.

3D-printed optogenetic neural probe integrated with microfluidic tube for opsin/drug delivery.

3D-printed optogenetic neural probe integrated with microfluidic tube for opsin/drug delivery.

Optogenetics, known for its precision in neural stimulation, is integral to behavioral research, enabling the study of neural circuits involved in decision-making, memory, social interaction, and movement. Traditional methodologies require two separate surgeries: the first to deliver a viral vector containing the opsin gene to the targeted brain region, and the second to implant an opto-probe for light stimulation. This dual-step process increases the risk of tissue damage and misalignment between the injection and implantation sites. In this study, we present a 3D-printed multimodal optogenetic neural probe that combines light delivery and fluid injection into a single device. By integrating a commercially available microfluidic tube with a 3D-printed opto-probe, the device offers rapid and customizable assembly for diverse applications. The probe was implanted in the subthalamic nucleus of mice, enabling viral vector delivery and device implantation in a single procedure. Following viral expression, behavioral experiments demonstrated that optical stimulation increased travel distance and velocity, confirming effective neuronal activation. Immunohistochemistry analysis revealed successful expression of Channelrhodopsin-2 (ChR2(H134R)) through mCherry labeling of neurons, reduced astrocytic (GFAP) and microglial (ED1) activation around the implantation site, and preserved neuronal populations as confirmed by NeuN staining. These results highlight the device's biocompatibility, minimal inflammatory response, and suitability for long-term neural modulation, with potential applications in research and clinical settings.

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来源期刊
Scientific Reports
Scientific Reports Natural Science Disciplines-
CiteScore
7.50
自引率
4.30%
发文量
19567
审稿时长
3.9 months
期刊介绍: We publish original research from all areas of the natural sciences, psychology, medicine and engineering. You can learn more about what we publish by browsing our specific scientific subject areas below or explore Scientific Reports by browsing all articles and collections. Scientific Reports has a 2-year impact factor: 4.380 (2021), and is the 6th most-cited journal in the world, with more than 540,000 citations in 2020 (Clarivate Analytics, 2021). •Engineering Engineering covers all aspects of engineering, technology, and applied science. It plays a crucial role in the development of technologies to address some of the world''s biggest challenges, helping to save lives and improve the way we live. •Physical sciences Physical sciences are those academic disciplines that aim to uncover the underlying laws of nature — often written in the language of mathematics. It is a collective term for areas of study including astronomy, chemistry, materials science and physics. •Earth and environmental sciences Earth and environmental sciences cover all aspects of Earth and planetary science and broadly encompass solid Earth processes, surface and atmospheric dynamics, Earth system history, climate and climate change, marine and freshwater systems, and ecology. It also considers the interactions between humans and these systems. •Biological sciences Biological sciences encompass all the divisions of natural sciences examining various aspects of vital processes. The concept includes anatomy, physiology, cell biology, biochemistry and biophysics, and covers all organisms from microorganisms, animals to plants. •Health sciences The health sciences study health, disease and healthcare. This field of study aims to develop knowledge, interventions and technology for use in healthcare to improve the treatment of patients.
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