一种在辐射条件下对活细胞进行亚细胞分辨率原位观察的定量辐照显微镜平台。

IF 1.9 4区 工程技术 Q3 MICROSCOPY
Zhao Chen, Liang Li, Jianli Liu, Yufang Zhao, Chenguang Liu, Jian Liu
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引用次数: 0

摘要

为了研究空间环境下质子辐射对活细胞的影响以及解读低剂量累积辐射效应的生物学机制,本研究开发了微束辐照显微镜平台。该系统集成了一个10 MeV质子加速器和一个垂直微束线设计。采用质子-光子-电子转换和高速光电电路的超快单质子计数和辐射同步控制模块实现了端到端操作延迟273.5 ns的确定性辐照控制。与宽视场和共聚焦荧光显微镜相结合,该平台可以在定量细胞照射期间进行实时原位观察,促进低剂量情况下辐射诱导损伤模式和信号转导的机制研究。人类胚胎肾293T细胞的实验验证成功模拟了空间辐射环境:剂量依赖性DNA双链断裂(通过γ-H2AX聚焦可见)和辐射诱导的引发损伤传播的旁观者效应。这些结果使该平台成为空间辐射健康风险评估不可或缺的工具,同时为质子治疗研究提供了微尺度能量沉积动力学的基础见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A quantitative irradiation microscopy platform for in situ observation of living cells with subcellular resolution under radiation conditions.

To address the critical need for investigating proton radiation effects on living cells in space environments and deciphering biological mechanisms underlying low-dose cumulative radiation effects, this study developed a microbeam irradiation microscopy platform. The system integrates a 10 MeV proton accelerator with a vertical microbeam line design. An ultrafast single-proton counting and radiation synchronisation control module-employing proton-photon-electron conversion and high-speed photoelectric circuitry achieve deterministic irradiation control with an end-to-end operational delay of 273.5 ns. Coupled with wide-field and confocal fluorescence microscopy, the platform enables real-time in situ observation during quantitative cellular irradiation, facilitating mechanistic studies of radiation-induced damage patterns and signal transduction in low-dose scenarios. Experimental validation using human embryonic kidney 293T cells demonstrated successful simulation of space radiation environments: dose-dependent DNA double-strand breaks (visualised via γ-H2AX foci) and radiation-induced bystander effects triggering damage propagation. These results establish the platform as an indispensable tool for space radiation health risk assessment while providing foundational insights into microscale energy deposition dynamics for proton therapy research.

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来源期刊
Journal of microscopy
Journal of microscopy 工程技术-显微镜技术
CiteScore
4.30
自引率
5.00%
发文量
83
审稿时长
1 months
期刊介绍: The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.
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