Xin Yuan, Jiajie Li, Tang Deng, Li Yang, Yilun Wu, Liangchen Xiang, Fang Yuan, Xinrui Sun, Yi Xia, Yihong Yang, Yi Zhang, Fang Ma, Yan Wang
{"title":"精子唾液酸酶NEU1和NEU3是体外受精受精率的潜在预测因子：一项前瞻性队列研究。","authors":"Xin Yuan, Jiajie Li, Tang Deng, Li Yang, Yilun Wu, Liangchen Xiang, Fang Yuan, Xinrui Sun, Yi Xia, Yihong Yang, Yi Zhang, Fang Ma, Yan Wang","doi":"10.1007/s10815-025-03602-6","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to explore the roles of spermatozoal sialidase NEU1 and NEU3 in affecting the fertilization rate of in vitro fertilization (IVF), and whether sperm sialidase NEU1 and NEU3 can be used as predictors of IVF outcome.</p><p><strong>Material and methods: </strong>This was a prospective cohort study that collected semen samples from 194 IVF couples between January 2024 and April 2024. Patients were grouped based on oocyte maturity and routine semen analysis. Detection of sperm NEU1 and NEU3 was carried out in semen from the same ejaculate for IVF by using flow cytometry, followed by correlation analysis with fertilization rates.</p><p><strong>Results: </strong>NEU1 and NEU3 are independent indicators separate from regular semen parameters. With a metaphase II (MII) rate ≥80% and normal semen routine analysis, sperm NEU1 (P = 0.0300) and NEU3 (P = 0.0253) levels were significantly different demarcated by 80% fertilization rate. Lower NEU1 and NEU3 expression on sperm is associated with lower fertilization rates. ROC curve analyses displaying the diagnostic accuracy of NEU1 (P = 0.0306, AUC = 0.660) and NEU3 (P = 0.0259, AUC = 0.665). χ<sup>2</sup> test analyzes the fertilization rate and the different cut-off rate for NEU1 (χ<sup>2</sup> = 4.671, P = 0.0307) or NEU3 (χ<sup>2</sup> = 6.377, P = 0.0116).</p><p><strong>Conclusions: </strong>NEU1 and NEU3 on sperm can predict the IVF fertilization outcome in the group with oocyte MII rate ≥80%. The protein levels of NEU1 and NEU3 on sperm could be a new index for assisted reproductive technology (ART).</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Spermatozoal sialidases NEU1 and NEU3 are potential predictors for fertilization rate in IVF: a prospective cohort study.\",\"authors\":\"Xin Yuan, Jiajie Li, Tang Deng, Li Yang, Yilun Wu, Liangchen Xiang, Fang Yuan, Xinrui Sun, Yi Xia, Yihong Yang, Yi Zhang, Fang Ma, Yan Wang\",\"doi\":\"10.1007/s10815-025-03602-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>This study aimed to explore the roles of spermatozoal sialidase NEU1 and NEU3 in affecting the fertilization rate of in vitro fertilization (IVF), and whether sperm sialidase NEU1 and NEU3 can be used as predictors of IVF outcome.</p><p><strong>Material and methods: </strong>This was a prospective cohort study that collected semen samples from 194 IVF couples between January 2024 and April 2024. Patients were grouped based on oocyte maturity and routine semen analysis. Detection of sperm NEU1 and NEU3 was carried out in semen from the same ejaculate for IVF by using flow cytometry, followed by correlation analysis with fertilization rates.</p><p><strong>Results: </strong>NEU1 and NEU3 are independent indicators separate from regular semen parameters. With a metaphase II (MII) rate ≥80% and normal semen routine analysis, sperm NEU1 (P = 0.0300) and NEU3 (P = 0.0253) levels were significantly different demarcated by 80% fertilization rate. Lower NEU1 and NEU3 expression on sperm is associated with lower fertilization rates. ROC curve analyses displaying the diagnostic accuracy of NEU1 (P = 0.0306, AUC = 0.660) and NEU3 (P = 0.0259, AUC = 0.665). χ<sup>2</sup> test analyzes the fertilization rate and the different cut-off rate for NEU1 (χ<sup>2</sup> = 4.671, P = 0.0307) or NEU3 (χ<sup>2</sup> = 6.377, P = 0.0116).</p><p><strong>Conclusions: </strong>NEU1 and NEU3 on sperm can predict the IVF fertilization outcome in the group with oocyte MII rate ≥80%. The protein levels of NEU1 and NEU3 on sperm could be a new index for assisted reproductive technology (ART).</p>\",\"PeriodicalId\":15246,\"journal\":{\"name\":\"Journal of Assisted Reproduction and Genetics\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-08-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Assisted Reproduction and Genetics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s10815-025-03602-6\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Assisted Reproduction and Genetics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10815-025-03602-6","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Spermatozoal sialidases NEU1 and NEU3 are potential predictors for fertilization rate in IVF: a prospective cohort study.
Purpose: This study aimed to explore the roles of spermatozoal sialidase NEU1 and NEU3 in affecting the fertilization rate of in vitro fertilization (IVF), and whether sperm sialidase NEU1 and NEU3 can be used as predictors of IVF outcome.
Material and methods: This was a prospective cohort study that collected semen samples from 194 IVF couples between January 2024 and April 2024. Patients were grouped based on oocyte maturity and routine semen analysis. Detection of sperm NEU1 and NEU3 was carried out in semen from the same ejaculate for IVF by using flow cytometry, followed by correlation analysis with fertilization rates.
Results: NEU1 and NEU3 are independent indicators separate from regular semen parameters. With a metaphase II (MII) rate ≥80% and normal semen routine analysis, sperm NEU1 (P = 0.0300) and NEU3 (P = 0.0253) levels were significantly different demarcated by 80% fertilization rate. Lower NEU1 and NEU3 expression on sperm is associated with lower fertilization rates. ROC curve analyses displaying the diagnostic accuracy of NEU1 (P = 0.0306, AUC = 0.660) and NEU3 (P = 0.0259, AUC = 0.665). χ2 test analyzes the fertilization rate and the different cut-off rate for NEU1 (χ2 = 4.671, P = 0.0307) or NEU3 (χ2 = 6.377, P = 0.0116).
Conclusions: NEU1 and NEU3 on sperm can predict the IVF fertilization outcome in the group with oocyte MII rate ≥80%. The protein levels of NEU1 and NEU3 on sperm could be a new index for assisted reproductive technology (ART).
期刊介绍：
The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species.
The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.
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