Chunyun Fu, Junming Lu, Xiangjun Lu, Yanhua Feng, Huan Zhang, Wenting Tang, Qiang Huang, Ya Huang, Lishai Mo, Huiping Huang, Qifei Li, Jie Tan
{"title":"利用靶向新一代测序技术检测5021例急性呼吸道感染儿科住院患者的铜绿假单胞菌","authors":"Chunyun Fu, Junming Lu, Xiangjun Lu, Yanhua Feng, Huan Zhang, Wenting Tang, Qiang Huang, Ya Huang, Lishai Mo, Huiping Huang, Qifei Li, Jie Tan","doi":"10.1016/j.cca.2025.120529","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the infection status and clinical manifestations of Pseudomonas aeruginosa (P.a.) in hospitalized children with respiratory tract infections.</p><p><strong>Methods: </strong>Between April 2021 and November 2023, a total of 5,021 hospitalized children with acute respiratory tract infections were collected at the Guangxi Maternal and Child Health Hospital. tNGS was used to detect pathogens in their respiratory samples.</p><p><strong>Results: </strong>Among the 5,021 hospitalized children with acute respiratory tract infections, P.a. was detected in 113 cases, with a detection rate of 2.25 %. The infected children were mainly infants under 1 year old, and males were predominant. Among the 113 P.a. positive cases, there was 1 case of single P.a. infection and 112 cases of co-infection with other pathogens. In infection patterns, the P.a.-bacteria-virus co-infection is relatively common. A total of 53 different pathogens were identified in children with mixed P. aeruginosa infections, with Human Herpesvirus 5 (observed in 40 cases) being the most prevalent, followed by Acinetobacter baumannii (39 cases) and Mycoplasma pneumoniae (24 cases). Among children with P. aeruginosa infection, the most common respiratory complications included sinusitis, respiratory failure, and pulmonary consolidation. In contrast, the most frequent complications affecting other systems were electrolyte disturbances, gastrointestinal dysfunction, anemia, and myocardial damage. The median duration of hospitalization for the 113 children was 8 days. Out of 113 children with P.a. infection, 55 cases (48.67 %) required respiratory support and 18 cases (15.93 %) required intensive care unit (ICU) admission.</p><p><strong>Conclusion: </strong>The detection rate of P.a. in hospitalized children with respiratory tract infections was 2.25 %. Almost all hospitalized children with P.a. respiratory tract infections had co-infections with other pathogens. The median duration of hospitalization was 8 days. 15.93 % of hospitalized children with P.a. infection required intensive care.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120529"},"PeriodicalIF":2.9000,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Pseudomonas aeruginosa in 5,021 hospitalized pediatric patients presenting with acute respiratory tract infections utilizing targeted next-generation sequencing.\",\"authors\":\"Chunyun Fu, Junming Lu, Xiangjun Lu, Yanhua Feng, Huan Zhang, Wenting Tang, Qiang Huang, Ya Huang, Lishai Mo, Huiping Huang, Qifei Li, Jie Tan\",\"doi\":\"10.1016/j.cca.2025.120529\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study aimed to assess the infection status and clinical manifestations of Pseudomonas aeruginosa (P.a.) in hospitalized children with respiratory tract infections.</p><p><strong>Methods: </strong>Between April 2021 and November 2023, a total of 5,021 hospitalized children with acute respiratory tract infections were collected at the Guangxi Maternal and Child Health Hospital. tNGS was used to detect pathogens in their respiratory samples.</p><p><strong>Results: </strong>Among the 5,021 hospitalized children with acute respiratory tract infections, P.a. was detected in 113 cases, with a detection rate of 2.25 %. The infected children were mainly infants under 1 year old, and males were predominant. Among the 113 P.a. positive cases, there was 1 case of single P.a. infection and 112 cases of co-infection with other pathogens. In infection patterns, the P.a.-bacteria-virus co-infection is relatively common. A total of 53 different pathogens were identified in children with mixed P. aeruginosa infections, with Human Herpesvirus 5 (observed in 40 cases) being the most prevalent, followed by Acinetobacter baumannii (39 cases) and Mycoplasma pneumoniae (24 cases). Among children with P. aeruginosa infection, the most common respiratory complications included sinusitis, respiratory failure, and pulmonary consolidation. In contrast, the most frequent complications affecting other systems were electrolyte disturbances, gastrointestinal dysfunction, anemia, and myocardial damage. The median duration of hospitalization for the 113 children was 8 days. Out of 113 children with P.a. infection, 55 cases (48.67 %) required respiratory support and 18 cases (15.93 %) required intensive care unit (ICU) admission.</p><p><strong>Conclusion: </strong>The detection rate of P.a. in hospitalized children with respiratory tract infections was 2.25 %. Almost all hospitalized children with P.a. respiratory tract infections had co-infections with other pathogens. The median duration of hospitalization was 8 days. 15.93 % of hospitalized children with P.a. infection required intensive care.</p>\",\"PeriodicalId\":10205,\"journal\":{\"name\":\"Clinica Chimica Acta\",\"volume\":\" \",\"pages\":\"120529\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2026-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinica Chimica Acta\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.cca.2025.120529\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/6 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinica Chimica Acta","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.cca.2025.120529","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/6 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Detection of Pseudomonas aeruginosa in 5,021 hospitalized pediatric patients presenting with acute respiratory tract infections utilizing targeted next-generation sequencing.
Objective: This study aimed to assess the infection status and clinical manifestations of Pseudomonas aeruginosa (P.a.) in hospitalized children with respiratory tract infections.
Methods: Between April 2021 and November 2023, a total of 5,021 hospitalized children with acute respiratory tract infections were collected at the Guangxi Maternal and Child Health Hospital. tNGS was used to detect pathogens in their respiratory samples.
Results: Among the 5,021 hospitalized children with acute respiratory tract infections, P.a. was detected in 113 cases, with a detection rate of 2.25 %. The infected children were mainly infants under 1 year old, and males were predominant. Among the 113 P.a. positive cases, there was 1 case of single P.a. infection and 112 cases of co-infection with other pathogens. In infection patterns, the P.a.-bacteria-virus co-infection is relatively common. A total of 53 different pathogens were identified in children with mixed P. aeruginosa infections, with Human Herpesvirus 5 (observed in 40 cases) being the most prevalent, followed by Acinetobacter baumannii (39 cases) and Mycoplasma pneumoniae (24 cases). Among children with P. aeruginosa infection, the most common respiratory complications included sinusitis, respiratory failure, and pulmonary consolidation. In contrast, the most frequent complications affecting other systems were electrolyte disturbances, gastrointestinal dysfunction, anemia, and myocardial damage. The median duration of hospitalization for the 113 children was 8 days. Out of 113 children with P.a. infection, 55 cases (48.67 %) required respiratory support and 18 cases (15.93 %) required intensive care unit (ICU) admission.
Conclusion: The detection rate of P.a. in hospitalized children with respiratory tract infections was 2.25 %. Almost all hospitalized children with P.a. respiratory tract infections had co-infections with other pathogens. The median duration of hospitalization was 8 days. 15.93 % of hospitalized children with P.a. infection required intensive care.
期刊介绍：
The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)
Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells.
The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.
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