Gladys Medina, Jose Luis Zambrano, Carmen Luisa Loureiro, Domingo J Garzaro, Miguel Barrios, Rosa Alba Salas, Scott C Weaver, Flor H Pujol
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Eastern Equine Encephalitis Virus Exhibits More Charged Amino Acids in Its Envelope Proteins, Compared to Madariaga Virus.
Given the importance of eastern equine encephalitis virus (EEEV; complex lineage I) in veterinary and public health in North America, there is limited knowledge about lineages II, III, and IV, also known as Madariaga virus (MADV), which are prevalent in Central and South America. Because charge mutations in the envelope glycoproteins are critical to the emergence of Venezuelan equine encephalitis virus, we analyzed the structural polyproteins of Venezuelan MADV isolates and compared them with sequences of EEEV. Four substitutions involving positively charged residues were identified in the E3 (R44Q) and E2 (R205H/Q, K260T/A, and R310Q) proteins. Additionally, histidine residues were present in EEEV and absent in MADV: E2 (H82Y, H94Y, H114Q, and H181S) and E1 (H118Q). None of these amino acids were predicted to be under selective pressure or associated with a significant conformational change in the envelope proteins. However, some of these substitutions might still be associated with the virulence and pathogenicity of EEEV.
期刊介绍：
The American Journal of Tropical Medicine and Hygiene, established in 1921, is published monthly by the American Society of Tropical Medicine and Hygiene. It is among the top-ranked tropical medicine journals in the world publishing original scientific articles and the latest science covering new research with an emphasis on population, clinical and laboratory science and the application of technology in the fields of tropical medicine, parasitology, immunology, infectious diseases, epidemiology, basic and molecular biology, virology and international medicine.
The Journal publishes unsolicited peer-reviewed manuscripts, review articles, short reports, images in Clinical Tropical Medicine, case studies, reports on the efficacy of new drugs and methods of treatment, prevention and control methodologies,new testing methods and equipment, book reports and Letters to the Editor. Topics range from applied epidemiology in such relevant areas as AIDS to the molecular biology of vaccine development.
The Journal is of interest to epidemiologists, parasitologists, virologists, clinicians, entomologists and public health officials who are concerned with health issues of the tropics, developing nations and emerging infectious diseases. Major granting institutions including philanthropic and governmental institutions active in the public health field, and medical and scientific libraries throughout the world purchase the Journal.
Two or more supplements to the Journal on topics of special interest are published annually. These supplements represent comprehensive and multidisciplinary discussions of issues of concern to tropical disease specialists and health issues of developing countries
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