赤霉素诱导多刺梨突变体果实孤雌核。

IF 4.5 2区 生物学 Q1 PLANT SCIENCES
Rameshkumar Ramakrishnan, Udi Zurgil, Shamili Kanna, Danuše Tarkowská, Ondřej Novák, Miroslav Strnad, Noemi Tel-Zur, Yaron Sitrit
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引用次数: 0

摘要

摘要/ abstract摘要:分离到了一个刺梨单性果实突变体,揭示了GA在单性果实发育中的作用,该突变体受GID-GA20ox/ ga20ox遗传系统调控GA的合成/调控。通过对刺梨单性生殖突变体Beer Sheva1 (BS1)及其相关非单性生殖茎的研究,探讨了刺梨单性生殖果实发育的复杂动力学。BS1果实为研究刺梨果实发育的调控机制提供了一个独特的模型。我们假设BS1子房中赤霉素(GAs)水平的升高诱导了孤雌果实的发育。通过整合不同的方法,包括GA定量和表达分析,我们阐明了生物合成、分解代谢和调控GA基因在协调胚珠发育中的关键作用。值得注意的是,我们的研究揭示了GA生物合成和分解代谢基因之间的复杂相互作用,特别是GID1, GA20ox和GA20ox,它们显著影响BS1卵巢中的GA水平。内源性GAs的定量测定证实,与相关胚珠相比,BS1中GA1、GA3和GA4的生物活性水平更高,表明GAs在孤雌生殖中的核心作用。此外,将GA抑制剂多效唑(PBZ)应用于BS1花蕾,可使BS1果实恢复到含有活籽的祖先表型,从而验证了GA在种子发育中的关键作用。高通量rna测序分析共鉴定出BS1中7717个差异表达基因(DEGs),其中ga相关基因较多。总的来说,我们的发现揭示了控制刺梨单性果实发育的复杂激素调节网络,为未来的研究铺平了道路,旨在了解胚珠发育和商业上理想的无籽果实的发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Gibberellin-induced parthenocarpy in fruits of a prickly pear mutant.

Gibberellin-induced parthenocarpy in fruits of a prickly pear mutant.

Gibberellin-induced parthenocarpy in fruits of a prickly pear mutant.

Gibberellin-induced parthenocarpy in fruits of a prickly pear mutant.

Gibberellin-induced parthenocarpy in fruits of a prickly pear mutant.

Gibberellin-induced parthenocarpy in fruits of a prickly pear mutant.

Gibberellin-induced parthenocarpy in fruits of a prickly pear mutant.

Key message: A parthenocarpic fruit mutant of prickly pear was isolated, revealing the role of GAs in parthenocarpic fruit development which is controlled by the GID-GA20ox/GA2ox genetic system modulating GA biosynthesis/regulation. We explored the intricate dynamics of parthenocarpic fruit development in prickly pear Opuntia ficus-indica (Cactaceae) through the investigation of fruits of the Beer Sheva1 (BS1) a parthenocarpic mutant and its revertant non-parthenocarpic stems. BS1 fruits, characterized by parthenocarpy and enlarged unfertilized ovules, provide a unique model for investigating the regulatory mechanisms underlying fruit development in prickly pear. We hypothesized that elevated levels of gibberellins (GAs) in BS1 ovaries induce parthenocarpic fruit development. By integrating different approaches, including GA quantification and expression analysis of ovaries from BS1 and revertant flowers, we elucidated the pivotal role of biosynthetic, catabolic, and regulatory GA genes in orchestrating ovule development. Notably, our investigation revealed a complex interplay between GA biosynthesis and catabolic genes, particularly GID1, GA20ox, and GA2ox, which significantly influenced GA levels in BS1 ovaries. Quantification of endogenous GAs confirmed higher levels of bioactive GA1, GA3, and GA4 in BS1 compared to revertant ovules, indicating the central role of GAs in parthenocarpy. Furthermore, application of the GA inhibitor paclobutrazol (PBZ) to BS1 flower buds resulted in the reversion of BS1 fruits to the progenitor phenotype containing viable seeds, thereby validating the critical involvement of GAs in seed development. High-throughput RNA-sequencing analysis identified a total of 7717 differentially expressed genes (DEGs) in BS1, among them GA-related genes. Overall, our findings shed light on the complex hormonal regulatory network governing parthenocarpic fruit development in prickly pear, paving the way for future studies aiming at understanding ovule development and development of commercially desirable seedless fruits.

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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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