Xu Zhu, Zhongyi Chen, Bin Deng, Chunhao Yang, Fengkun Xiao
{"title":"水仙花提取物。通过NRF2途径减轻uvb诱导的HaCaT细胞氧化损伤。","authors":"Xu Zhu, Zhongyi Chen, Bin Deng, Chunhao Yang, Fengkun Xiao","doi":"10.1080/13880209.2025.2540348","DOIUrl":null,"url":null,"abstract":"<p><strong>Context: </strong><i>Buddleja officinalis</i> Maxim., a prominent medicinal plant from the Scrophulariaceae family, is extensively distributed throught China, Korea, Vietnam, and Myanmar. This phytotherapeutic agent has demonstrated significant clinical efficacy in treating various ophthalmic disorders, such as xerophthalmia, infectious conjunctivitis, keratopathies, corneal ulcerations, and neuropathic ocular pain.</p><p><strong>Objective: </strong>To explore the potential photoprotective effects of <i>Buddleja officinalis</i> Maxim. extract (BOE) against UVB-induced oxidative damage in human immortalized keratinocytes.</p><p><strong>Materials and methods: </strong>In this study, we analyzed BOE components using HPLC and evaluated scavenging activities against 2, 2'-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS), and Superoxide anion (O<sub>2</sub><sup>·-</sup>) through biochemical assays. Cell viability was assessed with cell counting kit-8 assay (CCK-8) and lactate dehydrogenase (LDH) kits, intracellular reactive oxygen species (ROS) levels were quantified by flow cytometry, and cellular antioxidant enzyme activities were determined using commercial assay kits. To investigate specific antioxidant pathways, qPCR and Western blot analyses were performed.</p><p><strong>Results: </strong>The biochemical assays demonstrated that BOE possesses significant antioxidant activity. Additionally, BOE significantly alleviated the adverse effects of UVB exposure (30 mJ/cm<sup>2</sup>), which were characterized by decreased cell viability, elevated LDH activity, increased intracellular ROS levels, reduced activities of superoxide dismutase (SOD), decreased the levels of reduced glutathione (GSH), and increased Malondialdehyde (MDA) content. And the BOE modulates the antioxidant defense pathway by upregulating the mRNA and protein expression levels of nuclear factor erythroid 2-related factor 2(NRF2), heme oxygenase-1 (HO-1), and NAD(P)H: quinone oxidoreductase 1 (NQO1).</p><p><strong>Discussion and conclusions: </strong>Our findings indicate that the photoprotective efficacy of BOE makes it a promising candidate for incorporation as a natural component in pharmaceutical and cosmetic formulations.</p>","PeriodicalId":19942,"journal":{"name":"Pharmaceutical Biology","volume":"63 1","pages":"567-581"},"PeriodicalIF":4.8000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12333036/pdf/","citationCount":"0","resultStr":"{\"title\":\"The extract of <i>buddleja officinalis</i> maxim. alleviates UVB-induced oxidative damage <i>via</i> the NRF2 pathway in HaCaT cells.\",\"authors\":\"Xu Zhu, Zhongyi Chen, Bin Deng, Chunhao Yang, Fengkun Xiao\",\"doi\":\"10.1080/13880209.2025.2540348\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Context: </strong><i>Buddleja officinalis</i> Maxim., a prominent medicinal plant from the Scrophulariaceae family, is extensively distributed throught China, Korea, Vietnam, and Myanmar. This phytotherapeutic agent has demonstrated significant clinical efficacy in treating various ophthalmic disorders, such as xerophthalmia, infectious conjunctivitis, keratopathies, corneal ulcerations, and neuropathic ocular pain.</p><p><strong>Objective: </strong>To explore the potential photoprotective effects of <i>Buddleja officinalis</i> Maxim. extract (BOE) against UVB-induced oxidative damage in human immortalized keratinocytes.</p><p><strong>Materials and methods: </strong>In this study, we analyzed BOE components using HPLC and evaluated scavenging activities against 2, 2'-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS), and Superoxide anion (O<sub>2</sub><sup>·-</sup>) through biochemical assays. Cell viability was assessed with cell counting kit-8 assay (CCK-8) and lactate dehydrogenase (LDH) kits, intracellular reactive oxygen species (ROS) levels were quantified by flow cytometry, and cellular antioxidant enzyme activities were determined using commercial assay kits. To investigate specific antioxidant pathways, qPCR and Western blot analyses were performed.</p><p><strong>Results: </strong>The biochemical assays demonstrated that BOE possesses significant antioxidant activity. Additionally, BOE significantly alleviated the adverse effects of UVB exposure (30 mJ/cm<sup>2</sup>), which were characterized by decreased cell viability, elevated LDH activity, increased intracellular ROS levels, reduced activities of superoxide dismutase (SOD), decreased the levels of reduced glutathione (GSH), and increased Malondialdehyde (MDA) content. And the BOE modulates the antioxidant defense pathway by upregulating the mRNA and protein expression levels of nuclear factor erythroid 2-related factor 2(NRF2), heme oxygenase-1 (HO-1), and NAD(P)H: quinone oxidoreductase 1 (NQO1).</p><p><strong>Discussion and conclusions: </strong>Our findings indicate that the photoprotective efficacy of BOE makes it a promising candidate for incorporation as a natural component in pharmaceutical and cosmetic formulations.</p>\",\"PeriodicalId\":19942,\"journal\":{\"name\":\"Pharmaceutical Biology\",\"volume\":\"63 1\",\"pages\":\"567-581\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2025-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12333036/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmaceutical Biology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/13880209.2025.2540348\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmaceutical Biology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/13880209.2025.2540348","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/7 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
The extract of buddleja officinalis maxim. alleviates UVB-induced oxidative damage via the NRF2 pathway in HaCaT cells.
Context: Buddleja officinalis Maxim., a prominent medicinal plant from the Scrophulariaceae family, is extensively distributed throught China, Korea, Vietnam, and Myanmar. This phytotherapeutic agent has demonstrated significant clinical efficacy in treating various ophthalmic disorders, such as xerophthalmia, infectious conjunctivitis, keratopathies, corneal ulcerations, and neuropathic ocular pain.
Objective: To explore the potential photoprotective effects of Buddleja officinalis Maxim. extract (BOE) against UVB-induced oxidative damage in human immortalized keratinocytes.
Materials and methods: In this study, we analyzed BOE components using HPLC and evaluated scavenging activities against 2, 2'-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS), and Superoxide anion (O2·-) through biochemical assays. Cell viability was assessed with cell counting kit-8 assay (CCK-8) and lactate dehydrogenase (LDH) kits, intracellular reactive oxygen species (ROS) levels were quantified by flow cytometry, and cellular antioxidant enzyme activities were determined using commercial assay kits. To investigate specific antioxidant pathways, qPCR and Western blot analyses were performed.
Results: The biochemical assays demonstrated that BOE possesses significant antioxidant activity. Additionally, BOE significantly alleviated the adverse effects of UVB exposure (30 mJ/cm2), which were characterized by decreased cell viability, elevated LDH activity, increased intracellular ROS levels, reduced activities of superoxide dismutase (SOD), decreased the levels of reduced glutathione (GSH), and increased Malondialdehyde (MDA) content. And the BOE modulates the antioxidant defense pathway by upregulating the mRNA and protein expression levels of nuclear factor erythroid 2-related factor 2(NRF2), heme oxygenase-1 (HO-1), and NAD(P)H: quinone oxidoreductase 1 (NQO1).
Discussion and conclusions: Our findings indicate that the photoprotective efficacy of BOE makes it a promising candidate for incorporation as a natural component in pharmaceutical and cosmetic formulations.
期刊介绍：
Pharmaceutical Biology will publish manuscripts describing the discovery, methods for discovery, description, analysis characterization, and production/isolation (including sources and surveys) of biologically-active chemicals or other substances, drugs, pharmaceutical products, or preparations utilized in systems of traditional medicine.
Topics may generally encompass any facet of natural product research related to pharmaceutical biology. Papers dealing with agents or topics related to natural product drugs are also appropriate (e.g., semi-synthetic derivatives). Manuscripts will be published as reviews, perspectives, regular research articles, and short communications. The primary criteria for acceptance and publication are scientific rigor and potential to advance the field.
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