{"title":"PCR-RFLP检测越南长白猪和杜洛克猪隐性致死等位基因。","authors":"Anh Phu Nam Bui, Nghiep Mai Nguyen, Quang Le","doi":"10.1177/10406387251318022","DOIUrl":null,"url":null,"abstract":"<p><p>Increasing the number of live-born piglets per litter is one of the major objectives of pig breeding programs. Single nucleotide polymorphisms (SNPs) in polynucleotide kinase-phosphatase (<i>PNKP</i>), RNA polymerase I subunit B (<i>POLR1B</i>), ribosome biogenesis (<i>URB1</i>), and transcriptional adaptors ADA2 (<i>TADA2A</i> genes are recessive lethal alleles that are associated with embryonic lethality, which affects the reproductive performance and animal welfare of pig populations). No assay is available to screen for the recessive haplotypes of these 4 SNPs in pig populations. We successfully developed and verified a rapid, simple, and cost-effective PCR-restriction-fragment length polymorphism (PCR-RFLP) assay for genotyping SNPs in <i>PNKP</i>, <i>POLR1B</i>, <i>URB1</i> genes in Landrace and <i>TADA2A</i> genes in Duroc pigs in Vietnam. Our PCR-RFLP approach could potentially be applied in screening programs on a larger scale to improve the reproductive performance of commercial pig populations.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"753-758"},"PeriodicalIF":1.1000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12331623/pdf/","citationCount":"0","resultStr":"{\"title\":\"PCR-RFLP assays to detect recessive lethal alleles in Landrace and Duroc pigs in Vietnam.\",\"authors\":\"Anh Phu Nam Bui, Nghiep Mai Nguyen, Quang Le\",\"doi\":\"10.1177/10406387251318022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Increasing the number of live-born piglets per litter is one of the major objectives of pig breeding programs. Single nucleotide polymorphisms (SNPs) in polynucleotide kinase-phosphatase (<i>PNKP</i>), RNA polymerase I subunit B (<i>POLR1B</i>), ribosome biogenesis (<i>URB1</i>), and transcriptional adaptors ADA2 (<i>TADA2A</i> genes are recessive lethal alleles that are associated with embryonic lethality, which affects the reproductive performance and animal welfare of pig populations). No assay is available to screen for the recessive haplotypes of these 4 SNPs in pig populations. We successfully developed and verified a rapid, simple, and cost-effective PCR-restriction-fragment length polymorphism (PCR-RFLP) assay for genotyping SNPs in <i>PNKP</i>, <i>POLR1B</i>, <i>URB1</i> genes in Landrace and <i>TADA2A</i> genes in Duroc pigs in Vietnam. Our PCR-RFLP approach could potentially be applied in screening programs on a larger scale to improve the reproductive performance of commercial pig populations.</p>\",\"PeriodicalId\":17579,\"journal\":{\"name\":\"Journal of Veterinary Diagnostic Investigation\",\"volume\":\" \",\"pages\":\"753-758\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12331623/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Diagnostic Investigation\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1177/10406387251318022\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Diagnostic Investigation","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1177/10406387251318022","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/7 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
PCR-RFLP assays to detect recessive lethal alleles in Landrace and Duroc pigs in Vietnam.
Increasing the number of live-born piglets per litter is one of the major objectives of pig breeding programs. Single nucleotide polymorphisms (SNPs) in polynucleotide kinase-phosphatase (PNKP), RNA polymerase I subunit B (POLR1B), ribosome biogenesis (URB1), and transcriptional adaptors ADA2 (TADA2A genes are recessive lethal alleles that are associated with embryonic lethality, which affects the reproductive performance and animal welfare of pig populations). No assay is available to screen for the recessive haplotypes of these 4 SNPs in pig populations. We successfully developed and verified a rapid, simple, and cost-effective PCR-restriction-fragment length polymorphism (PCR-RFLP) assay for genotyping SNPs in PNKP, POLR1B, URB1 genes in Landrace and TADA2A genes in Duroc pigs in Vietnam. Our PCR-RFLP approach could potentially be applied in screening programs on a larger scale to improve the reproductive performance of commercial pig populations.
期刊介绍：
The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.
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