槲皮素对PRV感染3D4/2细胞氧化应激及组蛋白乙酰化变化的调控机制

IF 3.5 3区 医学 Q3 IMMUNOLOGY
Microbial pathogenesis Pub Date : 2025-11-01 Epub Date: 2025-08-05 DOI:10.1016/j.micpath.2025.107958
Jiaxia Jiang, Yuheng Wei, Wen Zhao, Haifeng Yuan, Changqiao Huang, Xiaoli Yu, Yanhua Li, Liqun Jiang, Meilling Yu, Tingjun Hu, Qiuhua Wang
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引用次数: 0

摘要

病毒感染会对身体造成氧化应激损伤。槲皮素是一种广泛存在于多种植物中的类黄酮化合物,具有抗氧化作用。本文旨在探讨槲皮苷的抗氧化机制及其对组蛋白乙酰化的影响。用添加或不添加槲皮苷的伪狂犬病毒(PRV)感染3D4/2细胞,检测细胞活力和抗氧化酶活性。western blot分析氧化应激相关信号通路和组蛋白乙酰化变化。结果表明,槲皮素对PRV感染的3D4/2细胞具有保护作用,对PRV感染细胞中SOD(超氧化物歧化酶)、GPx(谷胱甘肽过氧化物酶)、CAT(过氧化氢酶)活性的影响不尽相同,槲皮素在一定浓度下可提高PRV感染细胞中AcH3、AcH4和Nrf2信号通路相关蛋白的表达水平。此外,槲皮素可以抑制PRV感染细胞中AKT磷酸化,进一步促进AMPK磷酸化水平和PPAR-γ蛋白表达水平。AMPK通路抑制剂阻断了槲皮素对Nrf2和HO1蛋白表达水平的促进作用;PPAR-γ抑制剂阻断了槲皮素对Nrf2的促进作用。结果表明,槲皮素可通过Nrf2通路调节PRV感染3D4/2细胞的组蛋白乙酰化,槲皮素可减轻PRV诱导3D42细胞的氧化应激,这与Nrf2、AMPK和PPAR-γ信号通路有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regulatory mechanism of quercitrin on oxidative stress and histone acetylation changes in PRV infected 3D4/2 cells.

Viral infection causes oxidative stress damage to the body. Quercitrin is a flavonoid compound that exists widely in many plants and it exhibits antioxidant properties. The aim of this work was to investigate the antioxidant mechanism of quercitrin and its effect on histone acetylation changes. 3D4/2 cells were infected by PRV (Pseudorabies virus) with or without quercitrin, cell viability and antioxidant enzyme activity were tested. In addition, oxidative stress-related signaling pathways and histone acetylation changes were analyzed by western blots. The results showed that quercitrin played a protective effect on PRV infected 3D4/2 cells, the effects of quercitrin on the activity of SOD (Superoxide Dismutase), GPx (Glutathione peroxidase), CAT (Catalase) in PRV infected cell were not the same, quercitrin could increase the proteins expression levels AcH3, AcH4 and Nrf2 signaling pathway related proteins of PRV infected cells at certain concentrations. In addition, quercitrin could inhibit AKT phosphorylation in PRV infected cells and further promote the phosphorylation levels of AMPK and PPAR-γ protein expression levels. Moreover, AMPK pathway inhibitors blocked the promoting effect of quercitrin on Nrf2 and HO1 protein expression levels; PPAR-γ inhibitors blocked the promoting effect of quercitrin on Nrf2. The findings demonstrated that quercitrin could regulate histone acetylation in PRV infected 3D4/2 cells through the Nrf2 pathway and quercitrin could alleviate oxidative stress in 3D42 cells induced by PRV, which is associated with the Nrf2, AMPK, and PPAR-γ signaling pathway.

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来源期刊
Microbial pathogenesis
Microbial pathogenesis 医学-免疫学
CiteScore
7.40
自引率
2.60%
发文量
472
审稿时长
56 days
期刊介绍: Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)
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