Qingling Liu, Xiangyu Cao, Mengge Chen, Xin Yue, Longlong Liu, Rong Yang, Bo Xu, Hongkuan Deng
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Isolation, whole-genome analysis, and anti-biofilm activity of carbapenem-resistant Enterobacteriaceae phage KP-BW9 and its lysin Lys-BW9.
Carbapenem-resistant Enterobacteriaceae (CRE) have been classified as "urgent priority pathogens" due to their high drug resistance and wide spread. Among them, carbapenem-resistant Escherichia coli (CREco) and carbapenem-resistant Klebsiella pneumoniae (CRKP) are the main types. Phages are naturally occurring viruses that can specifically infect and lyse host bacteria, making them an effective alternative to antibiotics. This study isolated and identified a CREco phage, KP-BW9. The sequencing of phage KP-BW9 showed a genome size of 59665 bp, a GC content of 56.67 %, and encoded 79 predicted proteins. It has a latent period and a lysis period, with strong adaptability to the environment, and a lysis rate of 41.67 % for CREco. Both phage KP-BW9 and lysin Lys-BW9 could effectively lyse Escherichia coli and Klebsiella pneumoniae, and significantly inhibit and eradicate the biofilm of their individual and mixed bacteria. As a novel anti-CRE phage, KP-BW9 offers an innovative solution for controlling multidrug-resistant bacterial infections.
期刊介绍：
Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports.
Research Areas Include:
-Pathogenesis
-Virulence factors
-Host susceptibility or resistance
-Immune mechanisms
-Identification, cloning and sequencing of relevant genes
-Genetic studies
-Viruses, prokaryotic organisms and protozoa
-Microbiota
-Systems biology related to infectious diseases
-Targets for vaccine design (pre-clinical studies)
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