中国一株具有肠道嗜性的新型强毒PRRSV-1亚型1的鉴定

IF 3.5 3区 医学 Q3 IMMUNOLOGY
Microbial pathogenesis Pub Date : 2025-11-01 Epub Date: 2025-08-08 DOI:10.1016/j.micpath.2025.107951
Keyi Liu, Kang Zhang, Xindong Wang, Zhiying Su, Wenbo Liu, Guochang Chen, Tongwei Ren, Jianguang Qin, Jiaqi Liu, Yanli Pang, Yeshi Yin, Yifeng Qin, Kang Ouyang, Ying Chen, Weijian Huang, Yingyi Wei, Zuzhang Wei
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引用次数: 0

摘要

猪繁殖与呼吸综合征病毒(PRRSV)引起母猪流产和仔猪呼吸系统异常症状。近年来,中国报告的PRRSV-1感染病例越来越多,但关于PRRSV-1亚型致病性的数据有限。本研究从中国南方某猪场出现呼吸窘迫和腹泻的仔猪肺组织中分离出一株PRRSV菌株GXFS20220129。系统发育分析表明,该分离株属于PRRSV-1亚型S1.1谱系,与中国主要流行的PRRSV-1毒株不同。序列同源性分析显示,GXFS20220129与PRRSV-1各亚型的相似度为77.4% ~ 90.1%,与PRRSV-1原型株LV的相似度最高(90.1%)。与其他PRRSV-1亚型相比,GXFS20220129在Nsp2区有20个氨基酸缺失(位置274 ~ 293)和18个氨基酸缺失(位置324 ~ 341)。感染GXFS20220129的仔猪表现出短暂的发热、咳嗽、腹泻和体重减轻,最终导致4头仔猪中有2头死亡。实时荧光定量PCR (qRT-PCR)结果显示,感染仔猪肺部和肠道组织的病毒载量相当。组织病理学分析显示明显的间质性肺炎和肠上皮结构的破坏。免疫组化分析证实肺和肠组织均存在PRRSV N蛋白。上述结果表明,GXFS20220129菌株毒力强,具有明显的肠道嗜性,可引起仔猪严重的临床表现和死亡率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of a novel, virulent PRRSV-1 subtype 1 strain with intestinal tropism in China.

Porcine reproductive and respiratory syndrome virus (PRRSV) causes abortions in sows and abnormal respiratory symptoms in piglets. Recently, reports of PRRSV-1 infection in China are growing, however, data on the pathogenicity of PRRSV-1 subtype 1 are limited. In this study, a PRRSV strain, namely GXFS20220129, was isolated from the lung tissues of piglets exhibiting respiratory distress and diarrhea on a pig farm in southern China. Phylogenetic analysis revealed that the isolate belongs to lineage S1.1 within subtype 1 PRRSV-1, and is distinct from the major epidemic strains of PRRSV-1 in China. Sequence identity analysis showed that GXFS20220129 shares 77.4 %-90.1 % similarity with various PRRSV-1 subtypes, with the highest similarity (90.1 %) to the PRRSV-1 prototype strain LV. Compared to other subtype 1 PRRSV-1 strains, GXFS20220129 has 20 amino acid deletions (positions 274-293) and 18 amino acid deletions (positions 324-341) in the Nsp2 region. Piglets infected with GXFS20220129 exhibited transient fever, coughing, diarrhea, and weight loss, ultimately resulting in the death of 2 out of 4 piglets. Quantitative real-time PCR (qRT-PCR) showed comparable viral loads in the lung and intestinal tissues of infected piglets. Histopathological analyses revealed significant interstitial pneumonia and disruption of intestinal epithelial structures. Immunohistochemical analysis confirmed the presence of PRRSV N protein in both lung and intestinal tissues. These findings indicate that the GXFS20220129 strain is virulent and has a distinct intestinal tropism, causing severe clinical manifestations and mortality in piglets.

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来源期刊
Microbial pathogenesis
Microbial pathogenesis 医学-免疫学
CiteScore
7.40
自引率
2.60%
发文量
472
审稿时长
56 days
期刊介绍: Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)
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