{"title":"用数学模型分析丹参对心肌损伤的昼夜节律作用机制。","authors":"Xiaoqian Huo, Liansheng Qiao, Jianing Zhang, Yanling Zhang, Jiwang Zhang","doi":"10.1016/j.fitote.2025.106782","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim of the study: </strong>Salvia miltiorrhiza Bunge (Danshen, SMB) is commonly used in the treatment of myocardial injury in cardiovascular and cerebrovascular diseases. In recent research, circadian rhythm disruption was identified as a potential reason of myocardial injury. In this study, we investigated the mechanism of SMB and its components exerting protective effect on myocardial injury through circadian rhythms pathway.</p><p><strong>Materials and methods: </strong>The myocardial injury protection and regulation mechanism of circadian rhythm of SMB was discovered in the myocardial injury model of SD rats. SMB and its components on circadian rhythm regulation was based on the rhythm gene curve fitting. Synergistic action of multiple SMB components in circadian rhythm regulation was analyzed by system dynamics modelling.</p><p><strong>Results: </strong>SMB improved hemorheological indicators. Pathway were significantly enriched in myocardial tissue of SMB-treated myocardial injury rats through module analysis of protein interaction network. Regulation of circadian gene Arntl and Per1 expression by SMB extracts was analyzed in transcriptomics and the results shows that the SMB extracts can adjust the rhythm to normal state. The effects of eight major SMB components on the gene expression were detected by rhythmic gene expression profiling in myocardial injury model of H9c2 cell. In system dynamics model, the effects of eight components on the downstream genes of the pathway were simulated by mathematical model.</p><p><strong>Conclusion: </strong>This study indicated that SMB regulated circadian gene expression and improve circadian rhythm disorders. Circadian rhythms were one of important mechanism of action for SMB to exert myocardial injury protective effect.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106782"},"PeriodicalIF":2.6000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mechanism analysis of Salvia miltiorrhiza Bunge (Danshen) on circadian rhythm for treating myocardial injury by mathematic model.\",\"authors\":\"Xiaoqian Huo, Liansheng Qiao, Jianing Zhang, Yanling Zhang, Jiwang Zhang\",\"doi\":\"10.1016/j.fitote.2025.106782\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim of the study: </strong>Salvia miltiorrhiza Bunge (Danshen, SMB) is commonly used in the treatment of myocardial injury in cardiovascular and cerebrovascular diseases. In recent research, circadian rhythm disruption was identified as a potential reason of myocardial injury. In this study, we investigated the mechanism of SMB and its components exerting protective effect on myocardial injury through circadian rhythms pathway.</p><p><strong>Materials and methods: </strong>The myocardial injury protection and regulation mechanism of circadian rhythm of SMB was discovered in the myocardial injury model of SD rats. SMB and its components on circadian rhythm regulation was based on the rhythm gene curve fitting. Synergistic action of multiple SMB components in circadian rhythm regulation was analyzed by system dynamics modelling.</p><p><strong>Results: </strong>SMB improved hemorheological indicators. Pathway were significantly enriched in myocardial tissue of SMB-treated myocardial injury rats through module analysis of protein interaction network. Regulation of circadian gene Arntl and Per1 expression by SMB extracts was analyzed in transcriptomics and the results shows that the SMB extracts can adjust the rhythm to normal state. The effects of eight major SMB components on the gene expression were detected by rhythmic gene expression profiling in myocardial injury model of H9c2 cell. In system dynamics model, the effects of eight components on the downstream genes of the pathway were simulated by mathematical model.</p><p><strong>Conclusion: </strong>This study indicated that SMB regulated circadian gene expression and improve circadian rhythm disorders. Circadian rhythms were one of important mechanism of action for SMB to exert myocardial injury protective effect.</p>\",\"PeriodicalId\":12147,\"journal\":{\"name\":\"Fitoterapia\",\"volume\":\" \",\"pages\":\"106782\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fitoterapia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.fitote.2025.106782\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fitoterapia","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.fitote.2025.106782","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/5 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Mechanism analysis of Salvia miltiorrhiza Bunge (Danshen) on circadian rhythm for treating myocardial injury by mathematic model.
Aim of the study: Salvia miltiorrhiza Bunge (Danshen, SMB) is commonly used in the treatment of myocardial injury in cardiovascular and cerebrovascular diseases. In recent research, circadian rhythm disruption was identified as a potential reason of myocardial injury. In this study, we investigated the mechanism of SMB and its components exerting protective effect on myocardial injury through circadian rhythms pathway.
Materials and methods: The myocardial injury protection and regulation mechanism of circadian rhythm of SMB was discovered in the myocardial injury model of SD rats. SMB and its components on circadian rhythm regulation was based on the rhythm gene curve fitting. Synergistic action of multiple SMB components in circadian rhythm regulation was analyzed by system dynamics modelling.
Results: SMB improved hemorheological indicators. Pathway were significantly enriched in myocardial tissue of SMB-treated myocardial injury rats through module analysis of protein interaction network. Regulation of circadian gene Arntl and Per1 expression by SMB extracts was analyzed in transcriptomics and the results shows that the SMB extracts can adjust the rhythm to normal state. The effects of eight major SMB components on the gene expression were detected by rhythmic gene expression profiling in myocardial injury model of H9c2 cell. In system dynamics model, the effects of eight components on the downstream genes of the pathway were simulated by mathematical model.
Conclusion: This study indicated that SMB regulated circadian gene expression and improve circadian rhythm disorders. Circadian rhythms were one of important mechanism of action for SMB to exert myocardial injury protective effect.
期刊介绍：
Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas:
1. Characterization of active ingredients of medicinal plants
2. Development of standardization method for bioactive plant extracts and natural products
3. Identification of bioactivity in plant extracts
4. Identification of targets and mechanism of activity of plant extracts
5. Production and genomic characterization of medicinal plants biomass
6. Chemistry and biochemistry of bioactive natural products of plant origin
7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.
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