{"title":"Hsa_circ_0013729通过调节MEF2D以依赖ceRNA和RBP的方式促进胃癌进展。","authors":"Huazhi Li, Shaofei Chen, Yangqing Zhong, Lingjia Meng","doi":"10.1007/s10528-025-11216-x","DOIUrl":null,"url":null,"abstract":"<p><p>Circular RNAs (circRNAs) are emerging as major regulatory factors in gastric cancer progression. Here, in addition to the regulatory role of hsa_circ_0013729, we also evaluated its biomarker potential in gastric cancer. RNA-Seq profiles were obtained from GSE194384 and GSE184882. 116 gastric cancer specimens and adjacent para- tumor gastric tissues were analyzed for the expression of hsa_circ_0013729. The functional (proliferative, migratory, and invasive) significance of hsa_circ_0013729 was evaluated by cell experiments. The competing theories of endogenous RNAs and RNA-binding proteins were used to explore the underlying mechanism. Analyses of GSE194384 and GSE184882 identified hsa_circ_0013729 as a dysregulated circRNA in gastric cancer. The quantification of hsa_circ_0013729 in our patient cohort revealed its upregulation, diagnostic significance (ROC-AUC = 0.94, 95% confidence interval: 0.9149 to 0.9718; p < 0.0001), and prognostic value with a hazard ratio of 2.65 in gastric cancer. Hsa_circ_0013729 was identified as a potential driver in gastric cancer cell proliferation, migration, and invasion. Hsa_circ_0013729 acted as a ceRNA for miR-361-3p and interfered with the binding between miR-361-3p and MEF2D. Hsa_circ_0013729 interacted with HNRNPIL1 to stabilize MEF2D mRNA.Hsa_circ_0013729 may promote gastric cancer via the miR-361-3p/MEF2D axis and HNRNPUL1/MEF2D axis, showing potential as a biomarker and therapeutic target.</p>","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hsa_circ_0013729 Promotes Gastric Cancer Progression by Regulating MEF2D in ceRNA- and RBP- Dependent Manners.\",\"authors\":\"Huazhi Li, Shaofei Chen, Yangqing Zhong, Lingjia Meng\",\"doi\":\"10.1007/s10528-025-11216-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Circular RNAs (circRNAs) are emerging as major regulatory factors in gastric cancer progression. Here, in addition to the regulatory role of hsa_circ_0013729, we also evaluated its biomarker potential in gastric cancer. RNA-Seq profiles were obtained from GSE194384 and GSE184882. 116 gastric cancer specimens and adjacent para- tumor gastric tissues were analyzed for the expression of hsa_circ_0013729. The functional (proliferative, migratory, and invasive) significance of hsa_circ_0013729 was evaluated by cell experiments. The competing theories of endogenous RNAs and RNA-binding proteins were used to explore the underlying mechanism. Analyses of GSE194384 and GSE184882 identified hsa_circ_0013729 as a dysregulated circRNA in gastric cancer. The quantification of hsa_circ_0013729 in our patient cohort revealed its upregulation, diagnostic significance (ROC-AUC = 0.94, 95% confidence interval: 0.9149 to 0.9718; p < 0.0001), and prognostic value with a hazard ratio of 2.65 in gastric cancer. Hsa_circ_0013729 was identified as a potential driver in gastric cancer cell proliferation, migration, and invasion. Hsa_circ_0013729 acted as a ceRNA for miR-361-3p and interfered with the binding between miR-361-3p and MEF2D. Hsa_circ_0013729 interacted with HNRNPIL1 to stabilize MEF2D mRNA.Hsa_circ_0013729 may promote gastric cancer via the miR-361-3p/MEF2D axis and HNRNPUL1/MEF2D axis, showing potential as a biomarker and therapeutic target.</p>\",\"PeriodicalId\":482,\"journal\":{\"name\":\"Biochemical Genetics\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-08-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical Genetics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10528-025-11216-x\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10528-025-11216-x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Hsa_circ_0013729 Promotes Gastric Cancer Progression by Regulating MEF2D in ceRNA- and RBP- Dependent Manners.
Circular RNAs (circRNAs) are emerging as major regulatory factors in gastric cancer progression. Here, in addition to the regulatory role of hsa_circ_0013729, we also evaluated its biomarker potential in gastric cancer. RNA-Seq profiles were obtained from GSE194384 and GSE184882. 116 gastric cancer specimens and adjacent para- tumor gastric tissues were analyzed for the expression of hsa_circ_0013729. The functional (proliferative, migratory, and invasive) significance of hsa_circ_0013729 was evaluated by cell experiments. The competing theories of endogenous RNAs and RNA-binding proteins were used to explore the underlying mechanism. Analyses of GSE194384 and GSE184882 identified hsa_circ_0013729 as a dysregulated circRNA in gastric cancer. The quantification of hsa_circ_0013729 in our patient cohort revealed its upregulation, diagnostic significance (ROC-AUC = 0.94, 95% confidence interval: 0.9149 to 0.9718; p < 0.0001), and prognostic value with a hazard ratio of 2.65 in gastric cancer. Hsa_circ_0013729 was identified as a potential driver in gastric cancer cell proliferation, migration, and invasion. Hsa_circ_0013729 acted as a ceRNA for miR-361-3p and interfered with the binding between miR-361-3p and MEF2D. Hsa_circ_0013729 interacted with HNRNPIL1 to stabilize MEF2D mRNA.Hsa_circ_0013729 may promote gastric cancer via the miR-361-3p/MEF2D axis and HNRNPUL1/MEF2D axis, showing potential as a biomarker and therapeutic target.
期刊介绍：
Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses.
Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication.
Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses.
Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods.
Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.
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