{"title":"线粒体靶向荧光探针在铁下垂和多发性肝损伤模型中成像HOCl。","authors":"Cailing Fan, Na Xiao, Yixing Shi, Yonghao Song, Lei Liu, Yuwei Wu, Keying Yang, Yongwei Limeng, Qinxi Dong, Yanan Gao, Wei Shu, Chaoyuan Zeng","doi":"10.1007/s00216-025-06028-2","DOIUrl":null,"url":null,"abstract":"<div><p>Ferroptosis exerts a significant influence on the pathogenesis, progression, and therapeutic outcomes of liver diseases. Mitochondria play pivotal roles in liver disease and are also an indispensable hub in the final implementation of ferroptosis. The overabundance of mitochondrial HOCl can result in the permeabilization of mitochondria, which can cause cell death and damage to liver tissue. Herein, we designed a fluorescent probe named <b>PTZ-PY</b> based on thiophenazine for HOCl rapid detection. <b>PTZ-PY</b> exhibits excellent sensitivity and high selectivity to HOCl, and the LOD of HOCl is 13 nM. <b>PTZ-PY</b> is capable of targeting mitochondria and effectively monitors fluctuations in endogenous HOCl concentrations within cellular and zebrafish models, and has been utilized to document the escalation of HOCl levels associated with ferroptosis. As an efficient tool for detecting HOCl, <b>PTZ-PY</b> has demonstrated remarkable potential in exploring functions of HOCl in physiological processes, suggesting that it holds a promising application prospect in related research fields.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 22","pages":"5077 - 5086"},"PeriodicalIF":3.8000,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A mitochondrial-targeted fluorescent probe for imaging HOCl in ferroptosis and multiple liver injury models\",\"authors\":\"Cailing Fan, Na Xiao, Yixing Shi, Yonghao Song, Lei Liu, Yuwei Wu, Keying Yang, Yongwei Limeng, Qinxi Dong, Yanan Gao, Wei Shu, Chaoyuan Zeng\",\"doi\":\"10.1007/s00216-025-06028-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Ferroptosis exerts a significant influence on the pathogenesis, progression, and therapeutic outcomes of liver diseases. Mitochondria play pivotal roles in liver disease and are also an indispensable hub in the final implementation of ferroptosis. The overabundance of mitochondrial HOCl can result in the permeabilization of mitochondria, which can cause cell death and damage to liver tissue. Herein, we designed a fluorescent probe named <b>PTZ-PY</b> based on thiophenazine for HOCl rapid detection. <b>PTZ-PY</b> exhibits excellent sensitivity and high selectivity to HOCl, and the LOD of HOCl is 13 nM. <b>PTZ-PY</b> is capable of targeting mitochondria and effectively monitors fluctuations in endogenous HOCl concentrations within cellular and zebrafish models, and has been utilized to document the escalation of HOCl levels associated with ferroptosis. As an efficient tool for detecting HOCl, <b>PTZ-PY</b> has demonstrated remarkable potential in exploring functions of HOCl in physiological processes, suggesting that it holds a promising application prospect in related research fields.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":462,\"journal\":{\"name\":\"Analytical and Bioanalytical Chemistry\",\"volume\":\"417 22\",\"pages\":\"5077 - 5086\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-08-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical and Bioanalytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00216-025-06028-2\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Bioanalytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00216-025-06028-2","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
A mitochondrial-targeted fluorescent probe for imaging HOCl in ferroptosis and multiple liver injury models
Ferroptosis exerts a significant influence on the pathogenesis, progression, and therapeutic outcomes of liver diseases. Mitochondria play pivotal roles in liver disease and are also an indispensable hub in the final implementation of ferroptosis. The overabundance of mitochondrial HOCl can result in the permeabilization of mitochondria, which can cause cell death and damage to liver tissue. Herein, we designed a fluorescent probe named PTZ-PY based on thiophenazine for HOCl rapid detection. PTZ-PY exhibits excellent sensitivity and high selectivity to HOCl, and the LOD of HOCl is 13 nM. PTZ-PY is capable of targeting mitochondria and effectively monitors fluctuations in endogenous HOCl concentrations within cellular and zebrafish models, and has been utilized to document the escalation of HOCl levels associated with ferroptosis. As an efficient tool for detecting HOCl, PTZ-PY has demonstrated remarkable potential in exploring functions of HOCl in physiological processes, suggesting that it holds a promising application prospect in related research fields.
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Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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