用于超灵敏可靠检测甲型流感病毒核蛋白的比例电化学发光传感器

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-08-06 DOI:10.1016/j.snb.2025.138476

Guotao Duan, Xiaoxia Gu, Xianwen Kan

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引用次数: 0

摘要

甲型流感病毒（FluA）具有高传染性，对人类健康和全球经济构成严重威胁。灵敏可靠的流感检测对及时预防、感染控制和流行病学调查至关重要。本文研制了一种电化学发光（ECL）免疫传感器，用于超灵敏、可靠地检测流感病毒核蛋白（FluA NP）。一步法合成的鲁米诺包封金属有机凝胶（luu -MOG）由于鲁米诺的聚集诱导发光（AIE），表现出无共聚物的高阳极ECL响应。制备了姜黄素修饰的碳聚合物纳米棒作为阴极发光材料。FluA NP的存在将cu - cpnr引入到Lu-MOG修饰电极上，导致cu - mog向cu - cpnr的共振能量传递（RET）导致阳极ECL信号（Ia）降低，而cu - cpnr的阴极ECL强度（Ic）增加。因此，FluA NP检测采用Ic/Ia比例，线性范围和检出限分别为1.0×10-14 - 1.0×10-7 g/mL和8.97 fg/mL。与单信号（阳极或阴极ECL响应）相比，基于比例策略的ECL传感器具有良好的检测性能（线性范围宽、LOD低、稳定性和重复性好），能够准确、可靠地测定实际样品中的FluA NP，可能为临床诊断中FluA NP的监测提供了一种有前景的方法。

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Ratiometric electrochemiluminescence sensor for ultrasensitive and reliable detection of Influenza A virus nucleoprotein

Influenza A virus (FluA) with high infectivity poses a serious threat to human health and the global economy. Sensitive and reliable detection of FluA is of vital importance for timely prevention, infection control, and epidemiological investigations. Herein, an electrochemiluminescence (ECL) immunosensor was developed for the ultrasensitive and reliable detection of FluA nucleoprotein (FluA NP). A metal-organic gels (MOG) encapsulated with luminol (Lu-MOG) synthesized through one-step method showed a coreactant-free high anodic ECL response due to the aggregation-induced emission (AIE) of the luminol. Carbon polymer nanorods modified with curcumin (Cur-CPNR) was prepared as a cathode luminophore. The presence of FluA NP introduced Cur-CPNR on Lu-MOG modified electrode, causing the decrease of anodic ECL signal (I_a) owing to the resonance energy transfer (RET) from Lu-MOG to Cur-CPNR, as well as the increase of cathodic ECL intensity (I_c) of Cur-CPNR. Therefore, a ratio of I_c/I_a was employed for FluA NP detection with a linear range and a limit of detection of 1.0×10^-14 - 1.0×10^-7g/mL and 8.97 fg/mL, respectively. Compared with single signal (anodic or cathodic ECL response), the present ratiometric strategy based ECL sensor possessed good assay performances (wide linear range, low LOD, as well as excellent stability and repeatability) and enabled it to accurately and reliably determine FluA NP in real samples, probably providing a promising way for the monitoring of FluA NP in clinical diagnostics.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.