Lucia Molina, Geoffrey Williams, Andrés de Errasti, Denita Hadziabdic, María Belén Pildain
{"title":"在巴塔哥尼亚的lenga （Nothofagus pumilio）森林中从ambrosia甲虫（Gnathotrupes spp.）和木蛾（Chilecomadia valdiviana）的走廊和身体中培养的真菌的多样性。","authors":"Lucia Molina, Geoffrey Williams, Andrés de Errasti, Denita Hadziabdic, María Belén Pildain","doi":"10.1080/00275514.2025.2522019","DOIUrl":null,"url":null,"abstract":"<p><p>Wood-boring insects play an important role in turnover of trees and biomass in temperate forests and interact with a functionally diverse mycobiome. However, the diversity and dynamics of ambrosia beetles, other wood-boring insects, and their fungi remain relatively poorly understood in the forests of temperate South America. Baseline knowledge of insect and fungal diversity is therefore needed to provide a foundation for understanding the potential future dynamics of these critically important ecosystems in the context of global change. This study aimed to document fungal diversity that could be obtained in culture from larvae, adults, and galleries of ambrosia beetles (<i>Gnathotrupes</i> spp.) and a carpenter moth (<i>Chilecomadia valdiviana</i>) from lenga (<i>Nothofagus pumilio</i>) in northwest Patagonia, Argentina. Long molecular barcodes from fungal cultures isolated from galleries, larvae, and adult insects were obtained using nanopore sequencing. Fungal assemblages associated with <i>Gnathotrupes</i> spp. (32 unique taxa) and <i>C. valdiviana</i> (17 unique taxa) differed in structure and composition but shared 11 distinct taxa. Differences were found between fungal assemblages associated with <i>C. valdiviana</i> gut tracts and galleries. Fungal assemblages found in galleries and insect bodies of <i>Gnathotrupes</i> varied among species, seasons, and health conditions of the host crown. Our results also showed that the ophiostomatoid fungi <i>Raffaelea</i> spp. and yeast <i>Cyberlindnera</i> sp. were commonly found with <i>Gnathotrupes</i> spp. whereas <i>Ambrosiozyma angophorae</i> and <i>Oidiodendron</i> sp. were found with <i>C. valdiviana</i>. Species of the blue stain fungi <i>Ophiostoma patagonicum, O. nothofagi</i>, an unidentified <i>Sporothrix</i> sp. and <i>Huntiella decorticans</i> were found with both beetles and moths, and <i>O. patagonicum</i> was the most frequently isolated species. This is the first comprehensive study of microbiota isolated from <i>Gnathotrupes</i> spp. and <i>C. valdiviana</i>.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"818-834"},"PeriodicalIF":2.6000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Diversity of fungi cultured from galleries and bodies of ambrosia beetles (<i>Gnathotrupes</i> spp.) and carpenter moths (<i>Chilecomadia valdiviana</i>) in lenga (<i>Nothofagus pumilio</i>) forests in Patagonia.\",\"authors\":\"Lucia Molina, Geoffrey Williams, Andrés de Errasti, Denita Hadziabdic, María Belén Pildain\",\"doi\":\"10.1080/00275514.2025.2522019\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Wood-boring insects play an important role in turnover of trees and biomass in temperate forests and interact with a functionally diverse mycobiome. However, the diversity and dynamics of ambrosia beetles, other wood-boring insects, and their fungi remain relatively poorly understood in the forests of temperate South America. Baseline knowledge of insect and fungal diversity is therefore needed to provide a foundation for understanding the potential future dynamics of these critically important ecosystems in the context of global change. This study aimed to document fungal diversity that could be obtained in culture from larvae, adults, and galleries of ambrosia beetles (<i>Gnathotrupes</i> spp.) and a carpenter moth (<i>Chilecomadia valdiviana</i>) from lenga (<i>Nothofagus pumilio</i>) in northwest Patagonia, Argentina. Long molecular barcodes from fungal cultures isolated from galleries, larvae, and adult insects were obtained using nanopore sequencing. Fungal assemblages associated with <i>Gnathotrupes</i> spp. (32 unique taxa) and <i>C. valdiviana</i> (17 unique taxa) differed in structure and composition but shared 11 distinct taxa. Differences were found between fungal assemblages associated with <i>C. valdiviana</i> gut tracts and galleries. Fungal assemblages found in galleries and insect bodies of <i>Gnathotrupes</i> varied among species, seasons, and health conditions of the host crown. Our results also showed that the ophiostomatoid fungi <i>Raffaelea</i> spp. and yeast <i>Cyberlindnera</i> sp. were commonly found with <i>Gnathotrupes</i> spp. whereas <i>Ambrosiozyma angophorae</i> and <i>Oidiodendron</i> sp. were found with <i>C. valdiviana</i>. Species of the blue stain fungi <i>Ophiostoma patagonicum, O. nothofagi</i>, an unidentified <i>Sporothrix</i> sp. and <i>Huntiella decorticans</i> were found with both beetles and moths, and <i>O. patagonicum</i> was the most frequently isolated species. This is the first comprehensive study of microbiota isolated from <i>Gnathotrupes</i> spp. and <i>C. valdiviana</i>.</p>\",\"PeriodicalId\":18779,\"journal\":{\"name\":\"Mycologia\",\"volume\":\" \",\"pages\":\"818-834\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/00275514.2025.2522019\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/4 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycologia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/00275514.2025.2522019","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/4 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}
Diversity of fungi cultured from galleries and bodies of ambrosia beetles (Gnathotrupes spp.) and carpenter moths (Chilecomadia valdiviana) in lenga (Nothofagus pumilio) forests in Patagonia.
Wood-boring insects play an important role in turnover of trees and biomass in temperate forests and interact with a functionally diverse mycobiome. However, the diversity and dynamics of ambrosia beetles, other wood-boring insects, and their fungi remain relatively poorly understood in the forests of temperate South America. Baseline knowledge of insect and fungal diversity is therefore needed to provide a foundation for understanding the potential future dynamics of these critically important ecosystems in the context of global change. This study aimed to document fungal diversity that could be obtained in culture from larvae, adults, and galleries of ambrosia beetles (Gnathotrupes spp.) and a carpenter moth (Chilecomadia valdiviana) from lenga (Nothofagus pumilio) in northwest Patagonia, Argentina. Long molecular barcodes from fungal cultures isolated from galleries, larvae, and adult insects were obtained using nanopore sequencing. Fungal assemblages associated with Gnathotrupes spp. (32 unique taxa) and C. valdiviana (17 unique taxa) differed in structure and composition but shared 11 distinct taxa. Differences were found between fungal assemblages associated with C. valdiviana gut tracts and galleries. Fungal assemblages found in galleries and insect bodies of Gnathotrupes varied among species, seasons, and health conditions of the host crown. Our results also showed that the ophiostomatoid fungi Raffaelea spp. and yeast Cyberlindnera sp. were commonly found with Gnathotrupes spp. whereas Ambrosiozyma angophorae and Oidiodendron sp. were found with C. valdiviana. Species of the blue stain fungi Ophiostoma patagonicum, O. nothofagi, an unidentified Sporothrix sp. and Huntiella decorticans were found with both beetles and moths, and O. patagonicum was the most frequently isolated species. This is the first comprehensive study of microbiota isolated from Gnathotrupes spp. and C. valdiviana.
期刊介绍：
International in coverage, Mycologia presents recent advances in mycology, emphasizing all aspects of the biology of Fungi and fungus-like organisms, including Lichens, Oomycetes and Slime Molds. The Journal emphasizes subjects including applied biology, biochemistry, cell biology, development, ecology, evolution, genetics, genomics, molecular biology, morphology, new techniques, animal or plant pathology, phylogenetics, physiology, aspects of secondary metabolism, systematics, and ultrastructure. In addition to research articles, reviews and short notes, Mycologia also includes invited papers based on presentations from the Annual Conference of the Mycological Society of America, such as Karling Lectures or Presidential Addresses.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
