IRF8和Pu.1在系统性红斑狼疮患者B细胞中的过表达。

IF 1.9 4区 医学 Q3 RHEUMATOLOGY
Lupus Pub Date : 2025-10-01 Epub Date: 2025-08-05 DOI:10.1177/09612033251366400
Amin Azizan, Elham Farhadi, Seyedeh Tahereh Faezi, Majid Alikhani, Ahmadreza Jamshidi, Mohammad Vodjgani, Mahdi Mahmoudi
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引用次数: 0

摘要

系统性红斑狼疮(SLE)是一种复杂的自身免疫性疾病,以免疫调节改变为特征,特别是涉及B细胞,表现出生存增加和发育失调。本研究旨在探讨IRF4, IRF8, SP1和PU.1在SLE患者B细胞中的表达,因为这些因子在B细胞的发育,功能和分化中起着关键作用。方法分离培养sb细胞,用抗igm活化。采用逆转录聚合酶链反应(RT-PCR)检测基线和B细胞活化后IRF4、IRF8、SP1和PU.1 mRNA的表达。分析转录因子表达与临床参数的相关性。结果在B细胞激活后,与基线不同,SLE患者的IRF4表达显著增加,各组之间没有变化。IRF8在活动性SLE中表达显著升高,激活后表达增加。SP1的表达在所有组和条件下都保持稳定。PU.1基因表达在活动性SLE中基线时较高,在B细胞活化后进一步升高。IRF4与IRF8呈正相关,PU.1与SP1呈正相关。PU.1表达与SLE疾病活动性指标相关。结论SLE患者B细胞中irf4、IRF8、PU.1表达发生改变。PU.1与SLEDAI和抗dsdna滴度呈正相关。这些发现强调了它们在B细胞SLE发病机制中的潜在作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Overexpression of IRF8 and Pu.1 in B cells of systemic lupus erythematosus patients.

BackgroundSystemic Lupus Erythematosus (SLE), a complex autoimmune disorder characterized by altered immune regulation, particularly involving B cells, which exhibit increased survival and developmental dysregulation. This study aimed to investigate the expression of IRF4, IRF8, SP1, and PU.1 in B cells of SLE patients, as these factors are known to play critical roles in the development, function, and differentiation of B cells.MethodsB cells were isolated, cultured, and activated using anti-IgM. The mRNA expression of IRF4, IRF8, SP1, and PU.1 was assessed using reverse transcription polymerase chain reaction (RT-PCR) at baseline and after B cell activation. Correlations between transcription factor expression and clinical parameters were analyzed.ResultsUpon B cell activation, IRF4 expression increased significantly in SLE patients, unlike at baseline, where no changes were observed between groups. IRF8 expression was significantly raised in active SLE and increased upon activation. SP1 expression remained stable across all groups and conditions. PU.1 gene expression was higher in active SLE at baseline and increased further upon B cell activation. Positive correlations were found between IRF4 and IRF8, as well as between PU.1 and SP1. PU.1 expression correlated with SLE disease activity indices.ConclusionIRF4, IRF8, and PU.1 expression in B cells is altered in SLE. PU.1 shows a positive correlation with SLEDAI and anti-dsDNA titers. These findings highlight their potential roles in the pathogenesis of SLE in B cells.

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来源期刊
Lupus
Lupus 医学-风湿病学
CiteScore
4.20
自引率
11.50%
发文量
225
审稿时长
1 months
期刊介绍: The only fully peer reviewed international journal devoted exclusively to lupus (and related disease) research. Lupus includes the most promising new clinical and laboratory-based studies from leading specialists in all lupus-related disciplines. Invaluable reading, with extended coverage, lupus-related disciplines include: Rheumatology, Dermatology, Immunology, Obstetrics, Psychiatry and Cardiovascular Research…
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