Adaptive Optics-Assisted Long-Term 3D Fluorescence and Intensity Diffraction Tomography for High Spatiotemporal Resolution Cellular Imaging (Laser Photonics Rev. 19(15)/2025)

Adaptive Optics-Assisted Long-Term 3D Multimodal Imaging

In Research Article 2402196, Peng Gao, Jiasong Sun, Chao Zuo, and co-workers propose AO-FIDT, a novel long-term, high-spatiotemporal-resolution 3D fluorescence and intensity diffraction tomography microscopy technique. This method employs an iterative ptychographic approach to decouple 3D RI and wavefront aberrations from unlabeled intensity images. By feeding real-time wavefront aberrations back into the system's PSF, it synchronously corrects 3D fluorescence results and improves reconstruction quality. As the first AO-assisted 3D dual-modal imaging application, AO-FIDT effectively addresses the traditional challenge of reconciling long-term imaging with high spatiotemporal resolution, representing a significant advancement in 3D dual-modal microscopy.