混合区域伊朗蜂胶纳米颗粒的抗胃癌活性：潜在的治疗应用

IF 1.5 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Current Therapeutic Research-clinical and Experimental Pub Date : 2025-01-01 DOI:10.1016/j.curtheres.2025.100806

Sara Aravand MSc , Azam J. Esfahani PhD , Nematollah Gheibi PhD , Saeideh G. Khoei PhD , Shaghayegh P. Dibazar MSc , Leila Zolghadr PhD , Hossein Ahmadpour_Yazdi PhD

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引用次数: 0

摘要

蜂胶由于其类黄酮、酚酸和酯类的存在，在治疗开发方面具有巨大的潜力。然而，其化学成分限制了其溶解度和生物可及性。在这里，我们合成了来自伊朗3个不同地区的反应性伊朗蜂胶纳米颗粒，代表了它们对AGS胃癌细胞的抗癌作用的首次比较研究。方法采集伊朗3个不同地区的蜂胶。采用Bosio法制备伊朗蜂胶提取物（IPE）。进行了定量和定性分析。利用探针超声法制备了伊朗蜂胶纳米颗粒。采用动态光散射(DLS)-Zetasizer法对ipn进行鉴定。接下来，通过MTT法测定IPNs在胃癌细胞株上的细胞存活率，分析IPNs的抗癌潜力。采用Annexin V/ fitc -碘化丙啶（PI）流式细胞术检测IPNs的凋亡活性。结果IPE分析显示主要成分为副香酸和咖啡酸。ipn的平均尺寸为8 ~ 15 nm，具有良好的稳定性和细胞摄取性。与IPE相比，IPNs在24和48 h后对AGS胃癌细胞的存活抑制作用更大，计算出IPE和IPNs作用24 h时的IC50值分别为76.55和43.26µg/ml， 48 h时的IC50值分别为63.26和12.14µg/ml。流式细胞术结果显示IPNs诱导的细胞凋亡明显大于对照细胞。结论IPNs在降低AGS细胞活力和增加凋亡方面比IPE更有效。这些结果表明ipn作为胃癌治疗的低毒性纳米载体的潜力，尽管需要进一步的体内研究来验证其治疗潜力并评估其药代动力学特性。

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Anti-Gastric Cancer Activity of Mixed-Region Iranian Propolis Nanoparticles: Potential Therapeutic Applications

Background

Propolis holds great potential in therapeutic development due to the presence of flavonoids, phenolic acids, and esters. However, its chemical composition has restricted its solubility and bioaccessibility. Here, we synthesized responsive Iranian propolis nanoparticles derived from 3 distinct regions of Iran, representing the first comparative investigation of their anticancer effects against AGS gastric cancer cells.

Methods

Propolis was collected from 3 different regions of Iran. Iranian propolis extract (IPE) was prepared using Bosio method. Quantitative and qualitative analyses were performed. Using the probe sonication, Iranian propolis nanoparticles (IPNs) were prepared. Identification tests of IPNs were performed with dynamic light scattering (DLS)-Zetasizer methods. Next, the anticancer potential of IPNs was analyzed by measuring the cell survival rate on the AGS gastric cancer cell line by MTT assay. Also, the IPNs apoptotic activity was evaluated using Annexin V/FITC-propidium iodide (PI) flow cytometry.

Results

Analysis of the IPE showed the presence of paracoumaric acid and caffeic acid predominantly. An average IPNs size was obtained from 8 to 15 nm with good stability and cellular uptake. Compared with IPE, IPNs showed a greater effect on AGS gastric cancer cell survival inhibition after 24 and 48 h. The IC50 values of cancer cells treated with IPE and IPNs were calculated as 76.55 and 43.26 µg/ml for 24 h and 63.26 and 12.14 µg/ml for 48 h respectively. The flow cytometry results showed that the apoptosis induced by IPNs was greater than the control cells.

Conclusions

Our study indicated that the IPNs can be more effective than IPE in reducing AGS cell viability and increasing apoptosis. These results suggest the potential of IPNs as low-toxicity nanocarriers for gastric cancer therapy, although further in vivo studies are required to validate their therapeutic potential and assess their pharmacokinetic properties.

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来源期刊

Current Therapeutic Research-clinical and Experimental 医学-药学

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

3 months

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