对印度尼西亚东爪哇2023年11月登革热暴发期间的分子和血清学诊断进行评估

IF 1.8 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-08-05 DOI:10.1016/j.diagmicrobio.2025.117050

Luthfiana Mutiara Sani , Kartika Senjarini , Salome Steinke , Dao Thi Huyen , Tran Thi Thu Hien , Rike Oktarianti , Tran Thi Thanh Huyen , Le Huu Song , Thirumalaisamy P. Velavan , Do Duc Anh , Truong Nhat My

{"title":"对印度尼西亚东爪哇2023年11月登革热暴发期间的分子和血清学诊断进行评估","authors":"Luthfiana Mutiara Sani , Kartika Senjarini , Salome Steinke , Dao Thi Huyen , Tran Thi Thu Hien , Rike Oktarianti , Tran Thi Thanh Huyen , Le Huu Song , Thirumalaisamy P. Velavan , Do Duc Anh , Truong Nhat My","doi":"10.1016/j.diagmicrobio.2025.117050","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Dengue virus (DENV) is a major public health concern in Indonesia, with all four serotypes contributing to recurrent outbreaks. This study evaluated molecular diagnostic tests for DENV and identified circulating serotypes despite limited national surveillance.</div></div><div><h3>Methods</h3><div>Clinically diagnosed dengue patients were recruited during the 2023 outbreak in Jember, Indonesia. Dengue was confirmed using Rapid Diagnostic Tests (RDTs) for NS1, IgM, IgG, and RT-PCR assays (Fast Track Diagnostics (FTD) and DENV in-house RT-qPCR), with diagnostic accuracy assessed using a composite reference standard. DENV serotypes were identified using Altona, CDC, and Serotype in-house RT-PCR.</div></div><div><h3>Results</h3><div>The FTD RT-PCR test showed higher sensitivity (100 %) than the DENV in-house test (78 %). All RDTs-positive cases were confirmed as DENV RNA-positive by RT-PCR. Serotyping detected all four DENV serotypes, with DENV-3/4 co-infections in seven patients. While the CDC and Serotype in-house tests effectively identified DENV-2, DENV-4, and DENV-3/4; DENV-1 was better detected by the Altona and Serotype in-house tests. The Altona test failed to detect DENV-4 in our study.</div></div><div><h3>Conclusions</h3><div>All four DENV serotypes were detected during the 2023 outbreak, with possible re-emergence of DENV-4. The FTD dengue test proved effective for confirming infections, though rapid serological tests remain a practical but less precise alternative.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117050"},"PeriodicalIF":1.8000,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of molecular and serological diagnostics for dengue during the 2023 outbreak in Jember, East Java, indonesia\",\"authors\":\"Luthfiana Mutiara Sani , Kartika Senjarini , Salome Steinke , Dao Thi Huyen , Tran Thi Thu Hien , Rike Oktarianti , Tran Thi Thanh Huyen , Le Huu Song , Thirumalaisamy P. Velavan , Do Duc Anh , Truong Nhat My\",\"doi\":\"10.1016/j.diagmicrobio.2025.117050\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Dengue virus (DENV) is a major public health concern in Indonesia, with all four serotypes contributing to recurrent outbreaks. This study evaluated molecular diagnostic tests for DENV and identified circulating serotypes despite limited national surveillance.</div></div><div><h3>Methods</h3><div>Clinically diagnosed dengue patients were recruited during the 2023 outbreak in Jember, Indonesia. Dengue was confirmed using Rapid Diagnostic Tests (RDTs) for NS1, IgM, IgG, and RT-PCR assays (Fast Track Diagnostics (FTD) and DENV in-house RT-qPCR), with diagnostic accuracy assessed using a composite reference standard. DENV serotypes were identified using Altona, CDC, and Serotype in-house RT-PCR.</div></div><div><h3>Results</h3><div>The FTD RT-PCR test showed higher sensitivity (100 %) than the DENV in-house test (78 %). All RDTs-positive cases were confirmed as DENV RNA-positive by RT-PCR. Serotyping detected all four DENV serotypes, with DENV-3/4 co-infections in seven patients. While the CDC and Serotype in-house tests effectively identified DENV-2, DENV-4, and DENV-3/4; DENV-1 was better detected by the Altona and Serotype in-house tests. The Altona test failed to detect DENV-4 in our study.</div></div><div><h3>Conclusions</h3><div>All four DENV serotypes were detected during the 2023 outbreak, with possible re-emergence of DENV-4. The FTD dengue test proved effective for confirming infections, though rapid serological tests remain a practical but less precise alternative.</div></div>\",\"PeriodicalId\":11329,\"journal\":{\"name\":\"Diagnostic microbiology and infectious disease\",\"volume\":\"113 4\",\"pages\":\"Article 117050\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-08-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diagnostic microbiology and infectious disease\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0732889325003736\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diagnostic microbiology and infectious disease","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0732889325003736","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

摘要

登革热病毒（DENV）是印度尼西亚的一个主要公共卫生问题，所有四种血清型都可导致反复暴发。本研究评估了DENV的分子诊断测试，并在国家监测有限的情况下确定了循环血清型。方法收集印度尼西亚2023年12月登革热疫情期间临床诊断的登革热患者。采用NS1、IgM、IgG快速诊断试验（RDTs）和RT-PCR（快速通道诊断（FTD）和DENV内部RT-qPCR）对登革热进行确诊，并使用复合参考标准评估诊断准确性。使用Altona， CDC和内部血清型RT-PCR鉴定DENV血清型。结果FTD RT-PCR检测的灵敏度（100%）高于DENV室内检测（78%）。所有rdt阳性病例均经RT-PCR证实为DENV rna阳性。血清分型检测到所有四种DENV血清型，7例患者中出现DENV-3/4合并感染。CDC和内部血清型检测有效识别出DENV-2、DENV-4和DENV-3/4；Altona和血清型室内检测对DENV-1检测效果较好。在我们的研究中，Altona试验未能检测到DENV-4。结论在2023年疫情期间检测到所有4种DENV血清型，并有可能再次出现DENV-4。虽然快速血清学检测仍然是一种实用但不太精确的替代方法，但FTD登革热检测证明对确认感染是有效的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Evaluation of molecular and serological diagnostics for dengue during the 2023 outbreak in Jember, East Java, indonesia

Background

Dengue virus (DENV) is a major public health concern in Indonesia, with all four serotypes contributing to recurrent outbreaks. This study evaluated molecular diagnostic tests for DENV and identified circulating serotypes despite limited national surveillance.

Methods

Clinically diagnosed dengue patients were recruited during the 2023 outbreak in Jember, Indonesia. Dengue was confirmed using Rapid Diagnostic Tests (RDTs) for NS1, IgM, IgG, and RT-PCR assays (Fast Track Diagnostics (FTD) and DENV in-house RT-qPCR), with diagnostic accuracy assessed using a composite reference standard. DENV serotypes were identified using Altona, CDC, and Serotype in-house RT-PCR.

Results

The FTD RT-PCR test showed higher sensitivity (100 %) than the DENV in-house test (78 %). All RDTs-positive cases were confirmed as DENV RNA-positive by RT-PCR. Serotyping detected all four DENV serotypes, with DENV-3/4 co-infections in seven patients. While the CDC and Serotype in-house tests effectively identified DENV-2, DENV-4, and DENV-3/4; DENV-1 was better detected by the Altona and Serotype in-house tests. The Altona test failed to detect DENV-4 in our study.

Conclusions

All four DENV serotypes were detected during the 2023 outbreak, with possible re-emergence of DENV-4. The FTD dengue test proved effective for confirming infections, though rapid serological tests remain a practical but less precise alternative.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.