Yu-Hong Lin, Cheng Chen, Shoupeng Wei, Guillot Adrien, Bryan Mackowiak, Hongna Pan, Yaojie Fu, Luca Maccioni, Tianyi Ren, Li Zhang, Joseph Hibbeln, Robert Pawlosky, Bin Gao
{"title":"用气相色谱/质谱法测定小鼠体内乙醛和乙醇的高通量定量。","authors":"Yu-Hong Lin, Cheng Chen, Shoupeng Wei, Guillot Adrien, Bryan Mackowiak, Hongna Pan, Yaojie Fu, Luca Maccioni, Tianyi Ren, Li Zhang, Joseph Hibbeln, Robert Pawlosky, Bin Gao","doi":"10.1111/acer.70126","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Acetaldehyde, an immediate ethanol metabolite, mediates many ethanol-induced behavioral effects and is both psychoactive and toxic to animals and humans. Monitoring the kinetics of acetaldehyde using rodent models of alcohol misuse is essential for understanding and managing ethanol-associated diseases. However, quantitation of acetaldehyde in biological specimens after alcohol consumption has been challenging due to its high volatility, relatively low concentrations, and strong reactivity toward biochemical molecules. It was necessary to develop and establish an accurate and high-throughput method to quantitate acetaldehyde and ethanol.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Gas chromatography/mass spectrometry in positive chemical ionization mode coupled with a 111-vial headspace autosampler was employed to quantitate acetaldehyde and ethanol using <sup>2</sup>H<sub>4</sub>-acetaldehyde and <sup>2</sup>H<sub>5</sub>-ethanol as internal standards. A multidimensional approach was used to develop the method, including sample collection and processing, instrumental data analysis, optimization, and validation. Blood and tissues collected from genetically modified mouse models and their wild-type counterparts were studied.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The method was validated and applied to quantitate acetaldehyde and ethanol in blood and tissues from multiple mouse studies on ethanol metabolism. Acetaldehyde and ethanol were well-resolved from chromatographic interferences with linear ranges of 6.25–800 μM for acetaldehyde and 1.25–160 mM for ethanol. Both regression coefficients for calibration curves were >0.999. The within- and between-run precisions for ethanol in plasma, whole blood, and serum were all <5.0%, and for acetaldehyde in plasma and serum were <9.0%, while in whole blood it was 19.2%. Sample throughput was on the order of 60 samples per 15 h daily, with a maximum of 111 per batch.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Despite some limitations, this validated method proved to be specific, accurate, and reproducible for high-throughput quantitation of acetaldehyde and ethanol in rodent plasma, whole blood, serum, and visceral organs.</p>\n </section>\n </div>","PeriodicalId":72145,"journal":{"name":"Alcohol (Hanover, York County, Pa.)","volume":"49 9","pages":"1897-1911"},"PeriodicalIF":2.7000,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/acer.70126","citationCount":"0","resultStr":"{\"title\":\"High-throughput quantitation of acetaldehyde and ethanol in mice using gas chromatography/mass spectrometry positive chemical ionization\",\"authors\":\"Yu-Hong Lin, Cheng Chen, Shoupeng Wei, Guillot Adrien, Bryan Mackowiak, Hongna Pan, Yaojie Fu, Luca Maccioni, Tianyi Ren, Li Zhang, Joseph Hibbeln, Robert Pawlosky, Bin Gao\",\"doi\":\"10.1111/acer.70126\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Acetaldehyde, an immediate ethanol metabolite, mediates many ethanol-induced behavioral effects and is both psychoactive and toxic to animals and humans. Monitoring the kinetics of acetaldehyde using rodent models of alcohol misuse is essential for understanding and managing ethanol-associated diseases. However, quantitation of acetaldehyde in biological specimens after alcohol consumption has been challenging due to its high volatility, relatively low concentrations, and strong reactivity toward biochemical molecules. It was necessary to develop and establish an accurate and high-throughput method to quantitate acetaldehyde and ethanol.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Gas chromatography/mass spectrometry in positive chemical ionization mode coupled with a 111-vial headspace autosampler was employed to quantitate acetaldehyde and ethanol using <sup>2</sup>H<sub>4</sub>-acetaldehyde and <sup>2</sup>H<sub>5</sub>-ethanol as internal standards. A multidimensional approach was used to develop the method, including sample collection and processing, instrumental data analysis, optimization, and validation. Blood and tissues collected from genetically modified mouse models and their wild-type counterparts were studied.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The method was validated and applied to quantitate acetaldehyde and ethanol in blood and tissues from multiple mouse studies on ethanol metabolism. Acetaldehyde and ethanol were well-resolved from chromatographic interferences with linear ranges of 6.25–800 μM for acetaldehyde and 1.25–160 mM for ethanol. Both regression coefficients for calibration curves were >0.999. The within- and between-run precisions for ethanol in plasma, whole blood, and serum were all <5.0%, and for acetaldehyde in plasma and serum were <9.0%, while in whole blood it was 19.2%. 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High-throughput quantitation of acetaldehyde and ethanol in mice using gas chromatography/mass spectrometry positive chemical ionization
Background
Acetaldehyde, an immediate ethanol metabolite, mediates many ethanol-induced behavioral effects and is both psychoactive and toxic to animals and humans. Monitoring the kinetics of acetaldehyde using rodent models of alcohol misuse is essential for understanding and managing ethanol-associated diseases. However, quantitation of acetaldehyde in biological specimens after alcohol consumption has been challenging due to its high volatility, relatively low concentrations, and strong reactivity toward biochemical molecules. It was necessary to develop and establish an accurate and high-throughput method to quantitate acetaldehyde and ethanol.
Methods
Gas chromatography/mass spectrometry in positive chemical ionization mode coupled with a 111-vial headspace autosampler was employed to quantitate acetaldehyde and ethanol using 2H4-acetaldehyde and 2H5-ethanol as internal standards. A multidimensional approach was used to develop the method, including sample collection and processing, instrumental data analysis, optimization, and validation. Blood and tissues collected from genetically modified mouse models and their wild-type counterparts were studied.
Results
The method was validated and applied to quantitate acetaldehyde and ethanol in blood and tissues from multiple mouse studies on ethanol metabolism. Acetaldehyde and ethanol were well-resolved from chromatographic interferences with linear ranges of 6.25–800 μM for acetaldehyde and 1.25–160 mM for ethanol. Both regression coefficients for calibration curves were >0.999. The within- and between-run precisions for ethanol in plasma, whole blood, and serum were all <5.0%, and for acetaldehyde in plasma and serum were <9.0%, while in whole blood it was 19.2%. Sample throughput was on the order of 60 samples per 15 h daily, with a maximum of 111 per batch.
Conclusions
Despite some limitations, this validated method proved to be specific, accurate, and reproducible for high-throughput quantitation of acetaldehyde and ethanol in rodent plasma, whole blood, serum, and visceral organs.
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