探索阿尔茨海默病血浆生物标志物在-20°C下长时间储存的稳定性：对资源受限环境的影响

IF 3.1 3区医学 Q2 NEUROSCIENCES

Journal of Alzheimer's Disease Pub Date : 2025-10-01 Epub Date: 2025-08-03 DOI:10.1177/13872877251364899

Biniyam A Ayele, Patrice L Whitehead, Julianna Pascual, Tianjie Gu, Farid Rajabli, Jamie Arvizu, Charles G Golightly, Larry D Adams, Margaret A Pericak-Vance, Jeffery M Vance, Anthony J Griswold

{"title":"探索阿尔茨海默病血浆生物标志物在-20°C下长时间储存的稳定性：对资源受限环境的影响","authors":"Biniyam A Ayele, Patrice L Whitehead, Julianna Pascual, Tianjie Gu, Farid Rajabli, Jamie Arvizu, Charles G Golightly, Larry D Adams, Margaret A Pericak-Vance, Jeffery M Vance, Anthony J Griswold","doi":"10.1177/13872877251364899","DOIUrl":null,"url":null,"abstract":"Plasma samples for Alzheimer's disease biomarker analysis are ideally stored at -80°C, which is challenging in low research resource settings. We assessed the stability of pTau181, Aβ42, Aβ40, NfL, and GFAP in plasma stored at -20°C for 2, 4, 6, and 15 weeks using single molecule array assays. No significant variation was observed in pTau181 and Aβ42/Aβ40 ratio across all timepoints. NfL and GFAP showed greater variability. These findings indicate the stability of these Alzheimer's disease related analytes for up to 15 weeks at -20°C though future studies of other biomarkers and timeframes are recommended.","PeriodicalId":14929,"journal":{"name":"Journal of Alzheimer's Disease","volume":" ","pages":"973-979"},"PeriodicalIF":3.1000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring the stability of Alzheimer's disease plasma biomarkers stored for extended periods at -20°C: Implications for resource-constrained environments.\",\"authors\":\"Biniyam A Ayele, Patrice L Whitehead, Julianna Pascual, Tianjie Gu, Farid Rajabli, Jamie Arvizu, Charles G Golightly, Larry D Adams, Margaret A Pericak-Vance, Jeffery M Vance, Anthony J Griswold\",\"doi\":\"10.1177/13872877251364899\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Plasma samples for Alzheimer's disease biomarker analysis are ideally stored at -80°C, which is challenging in low research resource settings. We assessed the stability of pTau181, Aβ42, Aβ40, NfL, and GFAP in plasma stored at -20°C for 2, 4, 6, and 15 weeks using single molecule array assays. No significant variation was observed in pTau181 and Aβ42/Aβ40 ratio across all timepoints. NfL and GFAP showed greater variability. These findings indicate the stability of these Alzheimer's disease related analytes for up to 15 weeks at -20°C though future studies of other biomarkers and timeframes are recommended.\",\"PeriodicalId\":14929,\"journal\":{\"name\":\"Journal of Alzheimer's Disease\",\"volume\":\" \",\"pages\":\"973-979\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Alzheimer's Disease\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/13872877251364899\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/3 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Alzheimer's Disease","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/13872877251364899","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/3 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"NEUROSCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

用于阿尔茨海默病生物标志物分析的血浆样本理想地保存在-80°C，这在低研究资源环境中具有挑战性。我们使用单分子阵列法评估了pTau181、a - β42、a - β40、NfL和GFAP在-20°C保存2、4、6和15周的血浆中的稳定性。pTau181和a - β42/ a - β40比值在所有时间点均无显著变化。NfL和GFAP表现出更大的变异性。这些研究结果表明，这些与阿尔茨海默病相关的分析物在-20°C下的稳定性可达15周，但建议未来对其他生物标志物和时间框架进行研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Exploring the stability of Alzheimer's disease plasma biomarkers stored for extended periods at -20°C: Implications for resource-constrained environments.

Plasma samples for Alzheimer's disease biomarker analysis are ideally stored at -80°C, which is challenging in low research resource settings. We assessed the stability of pTau181, Aβ₄₂, Aβ₄₀, NfL, and GFAP in plasma stored at -20°C for 2, 4, 6, and 15 weeks using single molecule array assays. No significant variation was observed in pTau181 and Aβ₄₂/Aβ₄₀ ratio across all timepoints. NfL and GFAP showed greater variability. These findings indicate the stability of these Alzheimer's disease related analytes for up to 15 weeks at -20°C though future studies of other biomarkers and timeframes are recommended.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Alzheimer's Disease 医学-神经科学

CiteScore

6.40

自引率

7.50%

发文量

1327

审稿时长

2 months

期刊介绍： The Journal of Alzheimer''s Disease (JAD) is an international multidisciplinary journal to facilitate progress in understanding the etiology, pathogenesis, epidemiology, genetics, behavior, treatment and psychology of Alzheimer''s disease. The journal publishes research reports, reviews, short communications, hypotheses, ethics reviews, book reviews, and letters-to-the-editor. The journal is dedicated to providing an open forum for original research that will expedite our fundamental understanding of Alzheimer''s disease.