{"title":"通过SAP5抑制白色念珠菌和金黄色葡萄球菌双种界间生物膜发育。","authors":"Anmol Kulshrestha, Pratima Gupta","doi":"10.1080/17460913.2025.2539011","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>Microbial biofilms, particularly polymicrobial ones formed by <i>Candida albicans</i> and <i>Staphylococcus aureus</i>, exhibit increased resistance to conventional treatment. This study investigates the inhibitory effect of 4-hydroxyphenylacetic acid on Secreted Aspartyl Protease 5, a key virulence factor involved in hyphal development and biofilm formation in <i>Candida albicans</i>, to investigate its therapeutic potential in inhibiting polymicrobial biofilms developed with <i>Staphylococcus aureus</i>.</p><p><strong>Materials & methods: </strong>Biofilm formation was studied using a Biofilm Infection Simulator System and polymethylmethacrylate coupons. Biofilms were analyzed under untreated and 4-HPA-treated conditions. SAP5 proteinase activity on BSA was quantified in treated & un-treated cultures.</p><p><strong>Results: </strong>4-HPA treatment significantly restricted <i>C. albicans</i> hyphal growth, reducing <i>S. aureus</i> attachment. The biofilm decreased by over 78% in mono-species and 81% in polymicrobial cultures. The inhibition of SAP5 protease activity was 79% in mono-species and 65% in mixed cultures.</p><p><strong>Conclusions: </strong>4-HPA effectively reduces biofilm formation and SAP5 activity, highlighting its potential as a biofilm-specific therapeutic for polymicrobial infections.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"779-791"},"PeriodicalIF":2.4000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344808/pdf/","citationCount":"0","resultStr":"{\"title\":\"Control of <i>Candida albicans</i> and <i>Staphylococcus aureus</i> dual species interkingdom biofilm development via SAP5 inhibition.\",\"authors\":\"Anmol Kulshrestha, Pratima Gupta\",\"doi\":\"10.1080/17460913.2025.2539011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>Microbial biofilms, particularly polymicrobial ones formed by <i>Candida albicans</i> and <i>Staphylococcus aureus</i>, exhibit increased resistance to conventional treatment. This study investigates the inhibitory effect of 4-hydroxyphenylacetic acid on Secreted Aspartyl Protease 5, a key virulence factor involved in hyphal development and biofilm formation in <i>Candida albicans</i>, to investigate its therapeutic potential in inhibiting polymicrobial biofilms developed with <i>Staphylococcus aureus</i>.</p><p><strong>Materials & methods: </strong>Biofilm formation was studied using a Biofilm Infection Simulator System and polymethylmethacrylate coupons. Biofilms were analyzed under untreated and 4-HPA-treated conditions. SAP5 proteinase activity on BSA was quantified in treated & un-treated cultures.</p><p><strong>Results: </strong>4-HPA treatment significantly restricted <i>C. albicans</i> hyphal growth, reducing <i>S. aureus</i> attachment. The biofilm decreased by over 78% in mono-species and 81% in polymicrobial cultures. The inhibition of SAP5 protease activity was 79% in mono-species and 65% in mixed cultures.</p><p><strong>Conclusions: </strong>4-HPA effectively reduces biofilm formation and SAP5 activity, highlighting its potential as a biofilm-specific therapeutic for polymicrobial infections.</p>\",\"PeriodicalId\":12773,\"journal\":{\"name\":\"Future microbiology\",\"volume\":\" \",\"pages\":\"779-791\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344808/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Future microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/17460913.2025.2539011\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/3 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/17460913.2025.2539011","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/3 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Control of Candida albicans and Staphylococcus aureus dual species interkingdom biofilm development via SAP5 inhibition.
Aim: Microbial biofilms, particularly polymicrobial ones formed by Candida albicans and Staphylococcus aureus, exhibit increased resistance to conventional treatment. This study investigates the inhibitory effect of 4-hydroxyphenylacetic acid on Secreted Aspartyl Protease 5, a key virulence factor involved in hyphal development and biofilm formation in Candida albicans, to investigate its therapeutic potential in inhibiting polymicrobial biofilms developed with Staphylococcus aureus.
Materials & methods: Biofilm formation was studied using a Biofilm Infection Simulator System and polymethylmethacrylate coupons. Biofilms were analyzed under untreated and 4-HPA-treated conditions. SAP5 proteinase activity on BSA was quantified in treated & un-treated cultures.
Results: 4-HPA treatment significantly restricted C. albicans hyphal growth, reducing S. aureus attachment. The biofilm decreased by over 78% in mono-species and 81% in polymicrobial cultures. The inhibition of SAP5 protease activity was 79% in mono-species and 65% in mixed cultures.
Conclusions: 4-HPA effectively reduces biofilm formation and SAP5 activity, highlighting its potential as a biofilm-specific therapeutic for polymicrobial infections.
期刊介绍:
Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.