通过SAP5抑制白色念珠菌和金黄色葡萄球菌双种界间生物膜发育。

IF 2.4 4区 生物学 Q3 MICROBIOLOGY
Future microbiology Pub Date : 2025-08-01 Epub Date: 2025-08-03 DOI:10.1080/17460913.2025.2539011
Anmol Kulshrestha, Pratima Gupta
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引用次数: 0

摘要

目的:微生物生物膜,特别是由白色念珠菌和金黄色葡萄球菌形成的多微生物膜,对常规治疗表现出越来越强的耐药性。本研究通过研究4-羟基苯乙酸对分泌性天冬氨酸蛋白酶5的抑制作用,探讨其抑制金黄色葡萄球菌形成的多微生物生物膜的治疗潜力。天冬氨酸蛋白酶5是白色念珠菌菌丝发育和生物膜形成的关键毒力因子。材料与方法:利用生物膜感染模拟系统和聚甲基丙烯酸甲酯片研究生物膜的形成。在未经处理和4- hpa处理的条件下分析生物膜。在处理和未处理的培养物中定量测定牛血清白蛋白上SAP5蛋白酶的活性。结果:4-HPA处理明显抑制白色念珠菌菌丝生长,减少金黄色葡萄球菌附着。单菌种的生物膜减少了78%以上,多菌种的生物膜减少了81%。对SAP5蛋白酶活性的抑制在单种中为79%,在混合培养中为65%。结论:4-HPA可有效减少生物膜的形成和SAP5活性,突出了其作为生物膜特异性治疗多微生物感染的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Control of Candida albicans and Staphylococcus aureus dual species interkingdom biofilm development via SAP5 inhibition.

Aim: Microbial biofilms, particularly polymicrobial ones formed by Candida albicans and Staphylococcus aureus, exhibit increased resistance to conventional treatment. This study investigates the inhibitory effect of 4-hydroxyphenylacetic acid on Secreted Aspartyl Protease 5, a key virulence factor involved in hyphal development and biofilm formation in Candida albicans, to investigate its therapeutic potential in inhibiting polymicrobial biofilms developed with Staphylococcus aureus.

Materials & methods: Biofilm formation was studied using a Biofilm Infection Simulator System and polymethylmethacrylate coupons. Biofilms were analyzed under untreated and 4-HPA-treated conditions. SAP5 proteinase activity on BSA was quantified in treated & un-treated cultures.

Results: 4-HPA treatment significantly restricted C. albicans hyphal growth, reducing S. aureus attachment. The biofilm decreased by over 78% in mono-species and 81% in polymicrobial cultures. The inhibition of SAP5 protease activity was 79% in mono-species and 65% in mixed cultures.

Conclusions: 4-HPA effectively reduces biofilm formation and SAP5 activity, highlighting its potential as a biofilm-specific therapeutic for polymicrobial infections.

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来源期刊
Future microbiology
Future microbiology 生物-微生物学
CiteScore
4.90
自引率
3.20%
发文量
134
审稿时长
6-12 weeks
期刊介绍: Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.
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