诱导表达肝细胞GATA6（hiPSC-GATA6eGFP）的人诱导多能干细胞

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-07-27 DOI:10.1016/j.scr.2025.103784

Yan-Hua Luo , Jian-Yun Ge , Yue Chi , Min Ding , Hui-Ling Wu , Qingjian Zou , Yun-Wen Zheng

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引用次数: 0

摘要

为了解决将人诱导多能干细胞（hiPSCs）分化为均质肝细胞的传统策略的局限性，利用PiggyBac转座子系统开发了一种多西环素诱导的表达gata6的hiPSCs系。该hiPSC细胞系可以暂时控制GATA6的表达，使其在18天内同步分化为多系实质和非实质肝细胞。衍生的细胞表现出成熟的肝细胞特异性基因表达和持续的白蛋白分泌。该细胞系为生成功能性三维多谱系肝类器官提供了一个强大的平台，并为肝脏疾病的机制研究、疾病建模和再生医学提供了新的机会。

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Generation of human induced pluripotent stem cells with inducible expression of GATA6（Liver cells from hiPSC-GATA6eGFP）

To address the limitations of conventional strategies for differentiation human induced pluripotent stem cells (hiPSCs) into homogeneous hepatocytes, a doxycycline-inducible GATA6-expressing hiPSC line was developed using the PiggyBac transposon system. This hiPSC line permitted temporal control of GATA6 expression, enabling synchronous differentiation into multilineage parenchymal and non-parenchymal liver cells within 18 days. The derived cells demonstrated mature hepatocyte-specific gene expression and sustained albumin secretion. This cell line provides a robust platform for generating functional 3D multilineage liver organoids, and offers new opportunities for mechanistic studies of liver disorders, disease modeling, and regenerative medicine.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.