氢氧化钙与脂肪源性干细胞条件培养基（ADSCs-CM）联合通过RUNX-2和OPN表达对Wistar大鼠修复性牙本质形成的影响

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-08-05 DOI:10.1016/j.jobcr.2025.07.001

Radixtio Auzan Fepiosandi , Dian Agustin Wahjuningrum , Galih Sampoerno , Anuj Bhardwaj , Ajinkya M. Pawar

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引用次数: 0

摘要

背景：牙髓暴露需要有效的牙髓封盖，以保持牙髓的活力和功能。氢氧化钙（Ca(OH)2）被广泛用作纸浆封盖剂；然而，它有明显的局限性，包括形成具有隧道缺陷的修复性牙本质。这些缺陷可能会影响长期的成功。脂肪源性干细胞条件培养基（ADSCs-CM）通过旁分泌机制显示出再生潜力。目的探讨钙（OH）2联合ADSCs-CM对牙源性标志物RUNX-2和骨桥蛋白（OPN）表达的影响，并评价健康Wistar大鼠修复牙本质的组织学特征。材料与方法选用12只健康雌性褐家鼠（Rattus norvegicus）进行体内实验研究，随机分为对照组（仅Ca(OH)2）和处理组（Ca(OH)2 + ADSCs-CM按2:1比例），每组6只。V级空腔采用标准牙髓暴露制备。止血后，应用相应的材料，并用玻璃离子水门合剂密封。在第1、3、6天进行苏木精和伊红（HE）染色的组织学评估和RUNX-2和OPN表达的免疫组织化学评估。统计学分析采用Shapiro-Wilk检验和独立t检验(p <；0.05)。结果治疗组各时间点runx -2、OPN表达均显著升高(p <；0.05)，第6天达到峰值。组织学上，与对照组相比，治疗组表现出更均匀和更有组织的修复牙本质。结论Ca(OH)2中添加ADSCs-CM通过上调RUNX-2和OPN的表达促进修复性牙本质形成。这种联合治疗可以作为直接髓盖手术的生物学上有利的替代方法。

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Effect of calcium hydroxide and adipose-derived stem cell conditioned medium (ADSCs-CM) combination on reparative dentin formation in Wistar rats through RUNX-2 and OPN expression

Background

Vital pulp exposure requires effective pulp capping to preserve the vitality and function of the dental pulp. Calcium hydroxide (Ca(OH)₂) is widely used as a pulp capping agent; however, it has significant limitations, including the formation of reparative dentin with tunnel defects. These defects may compromise long-term success. Adipose-derived stem cell conditioned medium (ADSCs-CM) has shown regenerative potential through paracrine mechanisms.

Objectives

To evaluate the effect of combining Ca(OH)₂ with ADSCs-CM on the expression of odontogenic markers RUNX-2 and osteopontin (OPN), and secondarily to assess the histological characteristics of reparative dentin in healthy Wistar rats.

Materials and methods

An in vivo experimental study was conducted using twelve healthy female Wistar rats (Rattus norvegicus), randomly divided into control (Ca(OH)₂ only) and treatment (Ca(OH)₂ + ADSCs-CM in a 2:1 ratio) groups (n = 6/group). Class V cavities were prepared with standardized pulp exposure. Following hemostasis, the respective materials were applied and sealed with glass ionomer cement. Histological evaluation using hematoxylin and eosin (HE) staining and immunohistochemical assessment of RUNX-2 and OPN expression were performed on days 1, 3, and 6. Statistical analysis was conducted using the Shapiro-Wilk test and independent t-tests (p < 0.05).

Results

RUNX-2 and OPN expressions were significantly higher in the treatment group at all time points (p < 0.05), with peak levels observed on day 6. Histologically, the treatment group exhibited more homogeneous and better organized reparative dentin compared to the control group.

Conclusion

The addition of ADSCs-CM to Ca(OH)₂ enhances reparative dentinogenesis by upregulating RUNX-2 and OPN expression. This combination therapy may serve as a biologically favorable alternative for direct pulp capping procedures.

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.