将患者源性尿细胞重编程为iPSCs用于抗gad65自身免疫性脑炎研究。

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-07-28 DOI:10.1016/j.scr.2025.103790

Haribaskar Ramachandran , Saskia Räuber , Jochen Dobner , Barbara Hildebrandt , Denise Haslinger , Andreas G. Chiocchetti , Paul Disse , Lara-Maria Preuth , Rajeevan Narayanan Therpurakal , Sven G. Meuth , Nico Melzer , Andrea Rossi

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引用次数: 0

摘要

我们将一名抗gad65自身抗体相关自身免疫性边缘脑炎（ALE）患者的尿细胞重新编程为ipscine IUFi020-A。通过hipscore分析证实了多能性，而g带和cnv分析表明IUFi020-A具有真正的iPSC系的特征，与供体尿细胞相比没有遗传变化。strp分析进一步证实IUFi020-A来源于亲代尿液细胞。其他鉴定证实没有质粒整合和支原体污染。IUFi020-Aline提供了一种非侵入性的替代基于成纤维细胞的重编程方法，并可作为研究抗gad65相关ALE致病机制的有价值模型。

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Reprogramming Patient-Derived urine cells into iPSCs for Anti-GAD65 autoimmune encephalitis research

Urine cells from a patient with anti-GAD65 autoantibody-associated autoimmune limbic encephalitis (ALE) were reprogrammed into iPSC line IUFi020-A. Pluripotency was confirmed through hiPSCore analysis while G-banding and CNV analysis demonstrated that IUFi020-A exhibits characteristic features of a bona fide iPSC line with no genetic changes compared to the donor urine cells. STR analysis further confirmed that IUFi020-A was derived from the parental urine cells. Additional characterization verified the absence of plasmid integration and mycoplasma contamination. IUFi020-A line provides a non-invasive alternative to fibroblast-based reprogramming and serves as a valuable model for investigating the pathogenic mechanisms of anti-GAD65-associated ALE.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.