从异卵精子症患者的尿液中建立一种诱导多能干细胞系（LBMi001-A），该患者具有等双心Y染色体，具有完整的AZF区域。

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-07-29 DOI:10.1016/j.scr.2025.103793

Jiabin Wu , Yong Ma , Xiao Zhang, Yanhong Lin, Jian Zeng

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引用次数: 0

摘要

等心Y染色体[idic(Y)]是最常见的Y染色体畸变之一，导致性发育异常的广泛表型谱。本研究利用未整合的仙台病毒，从具有等双中心Y染色体、AZF区完整的少异生精子患者身上建立了首个尿源性诱导多能干细胞（iPSC）系。iPSC系保留了等心Y染色体，表达了多能性标记，并显示出三胚层分化的能力。这个iPSC模型具有相当大的前景，作为阐明idic(Y)相关男性不育症的分子和细胞机制的有力工具。

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Establishment of an induced pluripotent stem cell line (LBMi001-A) from urine of an oligoteratozoospermic patient with isodicentric chromosome Y harboring intact AZF region

Isodicentric Y chromosomes [idic(Y)] represent one of the most frequent Y chromosomal aberrations, leading to a wide phenotypic spectrum of abnormal sexual development. This study establishes the first urine-derived induced pluripotent stem cell (iPSC) line from an oligoteratozoospermic patient with isodicentric chromosome Y harboring intact AZF region using non-integrating Sendai virus. The iPSC line retained the isodicentric Y chromosome, expressed pluripotency markers, and displayed capability for differentiation of the three germ layers. This iPSC model holds considerable promise as a powerful tool for elucidating the molecular and cellular mechanisms underlying idic(Y)-associated male infertility.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.