Anya Lillemor Lindström Battle, Lee James Sweetlove
{"title":"多形地豆转基因表达的光遗传调控","authors":"Anya Lillemor Lindström Battle, Lee James Sweetlove","doi":"10.1002/aps3.11632","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Premise</h3>\n \n <p>The model liverwort <i>Marchantia polymorpha</i> is an emerging testbed species for plant metabolic engineering but lacks well-characterized inducible promoters, which are necessary to minimize biochemical and physiological disruption when over-accumulating target products. Here, we demonstrate the functionality of the light-inducible plant-usable light-switch elements (PULSE) optogenetic system in <i>Marchantia</i> and exemplify its use through the light-inducible overproduction of the bioplastic poly-3-hydroxybutyrate (PHB).</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>The PULSE system was used to drive expression of luciferase as a reporter and characterize its induction in transgenic <i>M. polymorpha</i>. Additionally, PULSE was used to drive expression of the PHB biosynthetic pathway; the accumulation of PHB under light-inducible control was compared to constitutive overexpression.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>PULSE was fully functional and minimally leaky in <i>M. polymorpha</i>. The presence of the PULSE construct, even in the absence of induction, resulted in a developmental phenotype. Constitutive and inducible expression resulted in similar PHB accumulation levels.</p>\n </section>\n \n <section>\n \n <h3> Discussion</h3>\n \n <p>PHB biosynthesis in plants is known to adversely affect plant health, but placing its production under optogenetic control alleviated negative effects on biomass accumulation in some instances. The work presented here represents a significant expansion of the toolbox for the metabolic engineering of <i>M. polymorpha</i>.</p>\n </section>\n </div>","PeriodicalId":8022,"journal":{"name":"Applications in Plant Sciences","volume":"13 4","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aps3.11632","citationCount":"0","resultStr":"{\"title\":\"Optogenetic control of transgene expression in Marchantia polymorpha\",\"authors\":\"Anya Lillemor Lindström Battle, Lee James Sweetlove\",\"doi\":\"10.1002/aps3.11632\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Premise</h3>\\n \\n <p>The model liverwort <i>Marchantia polymorpha</i> is an emerging testbed species for plant metabolic engineering but lacks well-characterized inducible promoters, which are necessary to minimize biochemical and physiological disruption when over-accumulating target products. Here, we demonstrate the functionality of the light-inducible plant-usable light-switch elements (PULSE) optogenetic system in <i>Marchantia</i> and exemplify its use through the light-inducible overproduction of the bioplastic poly-3-hydroxybutyrate (PHB).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>The PULSE system was used to drive expression of luciferase as a reporter and characterize its induction in transgenic <i>M. polymorpha</i>. Additionally, PULSE was used to drive expression of the PHB biosynthetic pathway; the accumulation of PHB under light-inducible control was compared to constitutive overexpression.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>PULSE was fully functional and minimally leaky in <i>M. polymorpha</i>. The presence of the PULSE construct, even in the absence of induction, resulted in a developmental phenotype. Constitutive and inducible expression resulted in similar PHB accumulation levels.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Discussion</h3>\\n \\n <p>PHB biosynthesis in plants is known to adversely affect plant health, but placing its production under optogenetic control alleviated negative effects on biomass accumulation in some instances. 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Optogenetic control of transgene expression in Marchantia polymorpha
Premise
The model liverwort Marchantia polymorpha is an emerging testbed species for plant metabolic engineering but lacks well-characterized inducible promoters, which are necessary to minimize biochemical and physiological disruption when over-accumulating target products. Here, we demonstrate the functionality of the light-inducible plant-usable light-switch elements (PULSE) optogenetic system in Marchantia and exemplify its use through the light-inducible overproduction of the bioplastic poly-3-hydroxybutyrate (PHB).
Methods
The PULSE system was used to drive expression of luciferase as a reporter and characterize its induction in transgenic M. polymorpha. Additionally, PULSE was used to drive expression of the PHB biosynthetic pathway; the accumulation of PHB under light-inducible control was compared to constitutive overexpression.
Results
PULSE was fully functional and minimally leaky in M. polymorpha. The presence of the PULSE construct, even in the absence of induction, resulted in a developmental phenotype. Constitutive and inducible expression resulted in similar PHB accumulation levels.
Discussion
PHB biosynthesis in plants is known to adversely affect plant health, but placing its production under optogenetic control alleviated negative effects on biomass accumulation in some instances. The work presented here represents a significant expansion of the toolbox for the metabolic engineering of M. polymorpha.
期刊介绍:
Applications in Plant Sciences (APPS) is a monthly, peer-reviewed, open access journal promoting the rapid dissemination of newly developed, innovative tools and protocols in all areas of the plant sciences, including genetics, structure, function, development, evolution, systematics, and ecology. Given the rapid progress today in technology and its application in the plant sciences, the goal of APPS is to foster communication within the plant science community to advance scientific research. APPS is a publication of the Botanical Society of America, originating in 2009 as the American Journal of Botany''s online-only section, AJB Primer Notes & Protocols in the Plant Sciences.
APPS publishes the following types of articles: (1) Protocol Notes describe new methods and technological advancements; (2) Genomic Resources Articles characterize the development and demonstrate the usefulness of newly developed genomic resources, including transcriptomes; (3) Software Notes detail new software applications; (4) Application Articles illustrate the application of a new protocol, method, or software application within the context of a larger study; (5) Review Articles evaluate available techniques, methods, or protocols; (6) Primer Notes report novel genetic markers with evidence of wide applicability.