油菜素内酯相关酰基转移酶1介导赤霉素信号和油菜素内酯之间的串扰,调节初级种子休眠。

IF 4.5 2区 生物学 Q1 PLANT SCIENCES
Sher Ney Chong, Pratibha Ravindran, Prakash P Kumar
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引用次数: 0

摘要

关键信息:RGL2:DOF6蛋白复合物增强BAT1表达,可能通过抑制油菜素内酯生物合成促进拟南芥种子初级休眠。因此,BAT1作为一种新的赤霉素和油菜素类固醇信号串扰中间体。包括RGA-LIKE2 (RGL2)和dna结合ONE ZINC FINGER6 (DOF6)在内的转录因子复合物有助于加强种子的初级休眠。为了阐明RGL2:DOF6复合物增强种子初级休眠的潜在机制,我们开始寻找该转录复合物的下游靶点。通过RNA序列分析,发现brassinosteroids相关的ACYLTRANSFERASE1 (BAT1)是RGL2:DOF6复合物的下游靶基因。利用突变系和过表达系进行了各种遗传和分子分析。BAT1是一种已知的内源性油菜素内酯(BR)水平调节剂。我们观察到新鲜收获的、未分层的bat1突变体种子发芽更早,而bat1过表达系比野生型(WT)种子发芽晚。同样,干燥贮藏和冷分层处理有助于打破WT种子的休眠,导致BAT1表达水平受到抑制。RT-qPCR和基于荧光素酶的转录分析显示,DOF6与赤霉素(GA)信号因子RGL2(即RGL2:DOF6复合物)联合可增强BAT1的表达。在脱落酸(ABA)处理的种子中,BAT1的表达也增强,表现为发芽延迟。BAT1维持种子初级休眠的功能可能是通过抑制BR生物合成来实现的,因为DOF6过表达的种子显示出BR生物合成基因(DWF1和ROT3)的抑制。总的来说,这些观察结果表明BAT1作为BR和GA信号通路之间的串扰中间体,是调节初代种子休眠的机制之一。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Brassinosteroid-related acyltransferase1 mediates crosstalk between gibberellin signaling and brassinosteroids to regulate primary seed dormancy.

Key message: RGL2:DOF6 protein complex enhances BAT1 expression, which promotes primary seed dormancy in Arabidopsis, likely by inhibiting brassinosteroid biosynthesis. Hence, BAT1 acts as a novel gibberellin and brassinosteroid signaling crosstalk intermediate. A transcription factor complex including RGA-LIKE2 (RGL2) and DNA-BINDING ONE ZINC FINGER6 (DOF6) helps enforce primary seed dormancy. To elucidate the underlying mechanism by which the RGL2:DOF6 complex can enhance primary seed dormancy, we initiated a search for the downstream targets of this transcription complex. Using RNA sequence analysis, BRASSINOSTEROID-RELATED ACYLTRANSFERASE1 (BAT1) was identified to be a downstream target gene of the RGL2:DOF6 complex. Various genetic and molecular analyses were conducted using the mutant and overexpression lines. BAT1 is a known modulator of endogenous brassinosteroid (BR) levels. We observed that freshly harvested, unstratified bat1 mutant seeds germinated earlier, while BAT1 overexpression lines germinated later than the wild-type (WT) seeds. Consistently, dry-storage and cold-stratification of WT seeds, treatments that help to break dormancy, caused suppression of BAT1 expression levels. RT-qPCR and luciferase-based transcriptional assays showed that BAT1 expression could be enhanced by DOF6 in combination with RGL2 (i.e., by RGL2:DOF6 complex), a gibberellin (GA) signaling factor. BAT1 expression was also enhanced in abscisic acid (ABA)-treated seeds, which exhibit delayed germination. The function of BAT1 in sustaining primary seed dormancy is likely to occur via inhibition of BR biosynthesis because DOF6 overexpression seeds show suppression of BR biosynthetic genes (DWF1 and ROT3). Overall, these observations reveal that BAT1 serves as a crosstalk intermediate between BR and GA signaling pathways as one of the mechanisms in the regulation of primary seed dormancy.

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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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