新弗朗西斯菌FTN_0096参与细胞内复制和宿主应答因子的鉴定

IF 2.6 3区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
PLoS ONE Pub Date : 2025-08-01 eCollection Date: 2025-01-01 DOI:10.1371/journal.pone.0329626
Dhandy Koesoemo Wardhana, Takashi Shimizu, Kenta Watanabe, Akihiko Uda, Masahisa Watarai
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引用次数: 0

摘要

土拉菌是人畜共患疾病土拉菌病的病原体。我们研究了土拉菌亚种的一个致病因子。novicida的词性:因此,我们利用HeLa细胞建立了一种新的感染模型。新发梭菌通常感染巨噬细胞系细胞,很少感染上皮细胞。我们成功地用含新乳弧菌抗体的小鼠血清补充了表达Fc受体的HeLa细胞(HeLa-Fcγ - rii细胞)。筛选了2232个转座子突变体,确定了对hela - fc - γ - rii细胞毒性相对较小的菌株,并分离出13株。转座子插入位点测序鉴定出FTN_0096等13个基因。我们专注于FTN_0096。尽管野生型菌株在hela - fc - γ rii和THP-1细胞中增殖,但细胞内FTN_0096突变体数量减少。FTN_0096突变体在感染初期不能从吞噬溶酶体中逃脱。此外,在线粒体和高尔基复合体中检测到FTN_0096突变体。这些结果表明,FTN_0096基因在细胞内复制中起着重要的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Identification of the Francisella novicida FTN_0096 as a factor involved in intracellular replication and host response.

Identification of the Francisella novicida FTN_0096 as a factor involved in intracellular replication and host response.

Identification of the Francisella novicida FTN_0096 as a factor involved in intracellular replication and host response.

Identification of the Francisella novicida FTN_0096 as a factor involved in intracellular replication and host response.

Francisella tularensis is the causative agent of the zoonotic disease tularemia. We investigated a pathogenic factor of F. tularensis subsp. novicida (F. novicida). Accordingly, we established a novel infection model using HeLa cells. F. novicida usually infects macrophage lineage cells and less frequently epithelial cells. We successfully infected HeLa cells expressing the Fc receptor (HeLa-FcγRII cells) using F. novicida supplemented with mouse serum containing F. novicida antibodies. A total of 2,232 transposon mutants of F. novicida were screened to determine the relatively fewer cytotoxic strains of the HeLa-FcγRII cells, and 13 strains were thus isolated. Sequencing analysis of transposon insertion sites identified 13 genes, including FTN_0096. We focused on FTN_0096. Although the F. novicida wild-type strain proliferated in HeLa-FcγRII and THP-1 cells, the number of intracellular FTN_0096 mutant decreased. FTN_0096 mutant cannot escape from phagolysosomes in the initial phases of infection. Moreover, FTN_0096 mutant was detected in the mitochondria and Golgi complex. These findings indicate the importance of FTN_0096 of F. novicida for intracellular replication in the cells.

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来源期刊
PLoS ONE
PLoS ONE 生物-生物学
CiteScore
6.20
自引率
5.40%
发文量
14242
审稿时长
3.7 months
期刊介绍: PLOS ONE is an international, peer-reviewed, open-access, online publication. PLOS ONE welcomes reports on primary research from any scientific discipline. It provides: * Open-access—freely accessible online, authors retain copyright * Fast publication times * Peer review by expert, practicing researchers * Post-publication tools to indicate quality and impact * Community-based dialogue on articles * Worldwide media coverage
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