儿童期系统性红斑狼疮的内毒素血症诱导低密度粒细胞和先天免疫细胞的细胞外陷阱。

IF 3.5 2区 医学 Q1 RHEUMATOLOGY
Wilasinee Saisorn, Pornpimol Phuengmaung, Chanunya Santiworakul, Kent Doi, Pornpimol Rianthavorn, Asada Leelahavanichkul
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引用次数: 0

摘要

背景:狼疮患者无感染的内毒素血症,其临床意义尚无定论。方法:将46例伴内毒素血症和狼疮活动度(系统性红斑狼疮疾病活动度指数2000评分)的儿童期狼疮患者分为伴内毒素血症的活动性狼疮(n=14)、伴内毒素血症的非活动性狼疮(n=10)、无内毒素血症的活动性狼疮(n=10)和无内毒素血症的非活动性狼疮(n=12)。常规指标(血肌酐、尿沉积物、蛋白尿、补体、血液学指标和组织活动指数)与狼疮活动度等指标进行分析。结果:血清细胞因子(肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、IL-8、IL-10)、瓜氨酸组蛋白H3、无细胞DNA、无细菌DNA各组间差异无统计学意义。骨髓过氧化物酶(MPO)和中性粒细胞弹性酶(NE)的免疫荧光检测显示,无论狼疮疾病的活动性如何,内毒素血症患者外周血单核细胞(PBMC)分数中的细胞外陷阱(ETs)均升高。有趣的是,低密度粒细胞(LDGs),即梯度分离后PBMC部分中的中性粒细胞,在与内毒素血症无关的活动性狼疮患者中升高,但在内毒素血症阳性患者中升高。由于内毒素血症可能来源于肠道,因此测量了血液微生物组,伯克霍尔德菌组是活动性狼疮内毒素血症的代表菌群。将LPS或无细菌DNA与健康对照组的中性粒细胞孵育后,这些常规密度的中性粒细胞转变为LDGs。结论:活动性和非活动性狼疮患者均存在内毒素血症(可能与肠道中的某些细菌群有关),引起PBMC部分和LDGs中的et。然而,LDGs升高在活动性狼疮内毒素血症中最为突出。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Endotoxaemia in childhood-onset systemic lupus erythematosus induces low-density granulocytes and extracellular traps of innate immune cells.

Endotoxaemia in childhood-onset systemic lupus erythematosus induces low-density granulocytes and extracellular traps of innate immune cells.

Endotoxaemia in childhood-onset systemic lupus erythematosus induces low-density granulocytes and extracellular traps of innate immune cells.

Endotoxaemia in childhood-onset systemic lupus erythematosus induces low-density granulocytes and extracellular traps of innate immune cells.

Background: Endotoxaemia without infection in lupus is mentioned with the inconclusive clinical importance.

Methods: With endotoxaemia and lupus activity (Systemic Lupus Erythematosus Disease Activity Index 2000 score), 46 patients with childhood-onset lupus were categorised into active lupus with endotoxaemia (n=14), inactive lupus with endotoxaemia (n=10), active lupus without endotoxaemia (n=10) and inactive lupus without endotoxaemia (n=12). The routine parameters (serum creatinine, urine sediments, proteinuria, complement, haematological aspects and histological activity index) were analysed with lupus activity and other parameters.

Results: Serum cytokines (tumour necrosis factor (TNF)-α, interleukin (IL)-6, IL-8 and IL-10), serum citrullinated histone H3, cell-free DNA and bacterial-free DNA were not different among groups. The extracellular traps (ETs) in the peripheral blood mononuclear cell (PBMC) fraction, measured by immunofluorescence of myeloperoxidase (MPO) and neutrophil elastase (NE), were elevated in endotoxaemia regardless of lupus disease activity. Interestingly, low-density granulocytes (LDGs), the neutrophils in the PBMC fraction after gradient separation, were elevated in active lupus regardless of endotoxaemia but higher in the patients with positive endotoxaemia. Because endotoxaemia might be derived from the gut, the blood microbiome was measured, and the Burkholderia group was the representative bacteria in active lupus with endotoxaemia. The incubation of LPS or bacterial-free DNA with neutrophils from the healthy control altered these regular-density neutrophils to LDGs.

Conclusion: Endotoxaemia presented in both active and inactive lupus (possibly correlated with some bacterial groups in the gut) that caused ETs in the PBMC fraction and LDGs. However, elevated LDGs were most prominent in endotoxaemia with active lupus.

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来源期刊
Lupus Science & Medicine
Lupus Science & Medicine RHEUMATOLOGY-
CiteScore
5.30
自引率
7.70%
发文量
88
审稿时长
15 weeks
期刊介绍: Lupus Science & Medicine is a global, peer reviewed, open access online journal that provides a central point for publication of basic, clinical, translational, and epidemiological studies of all aspects of lupus and related diseases. It is the first lupus-specific open access journal in the world and was developed in response to the need for a barrier-free forum for publication of groundbreaking studies in lupus. The journal publishes research on lupus from fields including, but not limited to: rheumatology, dermatology, nephrology, immunology, pediatrics, cardiology, hepatology, pulmonology, obstetrics and gynecology, and psychiatry.
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