技术说明:用液相色谱-串联质谱法(LC-MS/MS)测定类固醇激素的普通和血清分离采血管的比较:分析睾酮和脱氢表雄酮的血清分离采血管错误峰的观察。

IF 1 4区 医学 Q4 MEDICAL LABORATORY TECHNOLOGY
Uttam Garg, Bheemraj Ramoo, Clarence Frazee, Amitava Dasgupta
{"title":"技术说明:用液相色谱-串联质谱法(LC-MS/MS)测定类固醇激素的普通和血清分离采血管的比较:分析睾酮和脱氢表雄酮的血清分离采血管错误峰的观察。","authors":"Uttam Garg, Bheemraj Ramoo, Clarence Frazee, Amitava Dasgupta","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) is the gold standard for the analysis of steroids including 11-deoxycortisol, androstenedione, testosterone, 17-hydroxyprogesterone, and dehydroepiandrosterone (DHEA) in serum or plasma. In our hospital we observed an occasional problem in analysis of testosterone in some patients when blood was collected in Beckton-Dickinson serum separator gel tubes (BD gel tubes). Therefore, we investigated this issue.</p><p><strong>Methods: </strong>Blood was collected from 10 volunteers (five males and five females) simultaneously in plain red top tubes (no gel), Greiner serum separator gel tubes (Greiner gel tube), and BD (Beckton-Dickinson) gel tubes followed by extraction of steroids from serum, reconstitution with mobile phase, and subsequent analysis of various steroids using LC-MS/MS in a single run.</p><p><strong>Results: </strong>No interfering peak was observed when blood was collected in plain red top tube for all five steroids. However, we observed clinically significant interference with low testosterone values when blood specimens were collected in BD gel tubes. For DHEA, we observed interference when blood was collected in BD gel tubes and Griner tubes. Other steroids were not affected.</p><p><strong>Conclusions: </strong>We concluded that BD serum separator gel tube may not be suitable for analysis of testosterone in women and DHEA for both men and women.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 3","pages":"449-452"},"PeriodicalIF":1.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Technical Note:</i> Comparison of Plain and Serum-Separator Blood Collection Tubes for the Measurement of Steroid Hormones by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS): Observation of Erroneous Peaks in Serum Separator Tubes for Analysis of Testosterone and Dehydroepiandrosterone.\",\"authors\":\"Uttam Garg, Bheemraj Ramoo, Clarence Frazee, Amitava Dasgupta\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) is the gold standard for the analysis of steroids including 11-deoxycortisol, androstenedione, testosterone, 17-hydroxyprogesterone, and dehydroepiandrosterone (DHEA) in serum or plasma. In our hospital we observed an occasional problem in analysis of testosterone in some patients when blood was collected in Beckton-Dickinson serum separator gel tubes (BD gel tubes). Therefore, we investigated this issue.</p><p><strong>Methods: </strong>Blood was collected from 10 volunteers (five males and five females) simultaneously in plain red top tubes (no gel), Greiner serum separator gel tubes (Greiner gel tube), and BD (Beckton-Dickinson) gel tubes followed by extraction of steroids from serum, reconstitution with mobile phase, and subsequent analysis of various steroids using LC-MS/MS in a single run.</p><p><strong>Results: </strong>No interfering peak was observed when blood was collected in plain red top tube for all five steroids. However, we observed clinically significant interference with low testosterone values when blood specimens were collected in BD gel tubes. For DHEA, we observed interference when blood was collected in BD gel tubes and Griner tubes. Other steroids were not affected.</p><p><strong>Conclusions: </strong>We concluded that BD serum separator gel tube may not be suitable for analysis of testosterone in women and DHEA for both men and women.</p>\",\"PeriodicalId\":8228,\"journal\":{\"name\":\"Annals of clinical and laboratory science\",\"volume\":\"55 3\",\"pages\":\"449-452\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of clinical and laboratory science\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of clinical and laboratory science","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

目的:液相色谱-串联质谱联用(LC-MS/MS)是分析血清或血浆中甾体激素(11-脱氧皮质醇、雄烯二酮、睾酮、17-羟孕酮、脱氢表雄酮(DHEA))的金标准。在我院,我们观察到一些患者在使用贝克顿-迪金森血清分离凝胶管(BD凝胶管)采血时,偶有睾酮分析出现问题。因此,我们调查了这个问题。方法:10名志愿者(男5名,女5名)分别用普通红顶管(无凝胶)、Greiner血清分离凝胶管(Greiner凝胶管)和BD (Beckton-Dickinson)凝胶管同时采集血液,从血清中提取类固醇,流动相重构,随后用LC-MS/MS一次性分析各种类固醇。结果:5种类固醇素红顶管采血均未见干扰峰。然而,我们观察到在BD凝胶管中采集血液标本时,低睾酮值有临床意义的干扰。对于脱氢表雄酮,我们在BD凝胶管和Griner管中采集血液时观察到干扰。其他类固醇不受影响。结论:BD血清分离凝胶管可能不适合用于女性睾酮和男性和女性脱氢表雄酮的分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Technical Note: Comparison of Plain and Serum-Separator Blood Collection Tubes for the Measurement of Steroid Hormones by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS): Observation of Erroneous Peaks in Serum Separator Tubes for Analysis of Testosterone and Dehydroepiandrosterone.

Objective: Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) is the gold standard for the analysis of steroids including 11-deoxycortisol, androstenedione, testosterone, 17-hydroxyprogesterone, and dehydroepiandrosterone (DHEA) in serum or plasma. In our hospital we observed an occasional problem in analysis of testosterone in some patients when blood was collected in Beckton-Dickinson serum separator gel tubes (BD gel tubes). Therefore, we investigated this issue.

Methods: Blood was collected from 10 volunteers (five males and five females) simultaneously in plain red top tubes (no gel), Greiner serum separator gel tubes (Greiner gel tube), and BD (Beckton-Dickinson) gel tubes followed by extraction of steroids from serum, reconstitution with mobile phase, and subsequent analysis of various steroids using LC-MS/MS in a single run.

Results: No interfering peak was observed when blood was collected in plain red top tube for all five steroids. However, we observed clinically significant interference with low testosterone values when blood specimens were collected in BD gel tubes. For DHEA, we observed interference when blood was collected in BD gel tubes and Griner tubes. Other steroids were not affected.

Conclusions: We concluded that BD serum separator gel tube may not be suitable for analysis of testosterone in women and DHEA for both men and women.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Annals of clinical and laboratory science
Annals of clinical and laboratory science 医学-医学实验技术
CiteScore
1.60
自引率
0.00%
发文量
112
审稿时长
6-12 weeks
期刊介绍: The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信