{"title":"技术说明:用液相色谱-串联质谱法(LC-MS/MS)测定类固醇激素的普通和血清分离采血管的比较:分析睾酮和脱氢表雄酮的血清分离采血管错误峰的观察。","authors":"Uttam Garg, Bheemraj Ramoo, Clarence Frazee, Amitava Dasgupta","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) is the gold standard for the analysis of steroids including 11-deoxycortisol, androstenedione, testosterone, 17-hydroxyprogesterone, and dehydroepiandrosterone (DHEA) in serum or plasma. In our hospital we observed an occasional problem in analysis of testosterone in some patients when blood was collected in Beckton-Dickinson serum separator gel tubes (BD gel tubes). Therefore, we investigated this issue.</p><p><strong>Methods: </strong>Blood was collected from 10 volunteers (five males and five females) simultaneously in plain red top tubes (no gel), Greiner serum separator gel tubes (Greiner gel tube), and BD (Beckton-Dickinson) gel tubes followed by extraction of steroids from serum, reconstitution with mobile phase, and subsequent analysis of various steroids using LC-MS/MS in a single run.</p><p><strong>Results: </strong>No interfering peak was observed when blood was collected in plain red top tube for all five steroids. However, we observed clinically significant interference with low testosterone values when blood specimens were collected in BD gel tubes. For DHEA, we observed interference when blood was collected in BD gel tubes and Griner tubes. Other steroids were not affected.</p><p><strong>Conclusions: </strong>We concluded that BD serum separator gel tube may not be suitable for analysis of testosterone in women and DHEA for both men and women.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 3","pages":"449-452"},"PeriodicalIF":1.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Technical Note:</i> Comparison of Plain and Serum-Separator Blood Collection Tubes for the Measurement of Steroid Hormones by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS): Observation of Erroneous Peaks in Serum Separator Tubes for Analysis of Testosterone and Dehydroepiandrosterone.\",\"authors\":\"Uttam Garg, Bheemraj Ramoo, Clarence Frazee, Amitava Dasgupta\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) is the gold standard for the analysis of steroids including 11-deoxycortisol, androstenedione, testosterone, 17-hydroxyprogesterone, and dehydroepiandrosterone (DHEA) in serum or plasma. In our hospital we observed an occasional problem in analysis of testosterone in some patients when blood was collected in Beckton-Dickinson serum separator gel tubes (BD gel tubes). Therefore, we investigated this issue.</p><p><strong>Methods: </strong>Blood was collected from 10 volunteers (five males and five females) simultaneously in plain red top tubes (no gel), Greiner serum separator gel tubes (Greiner gel tube), and BD (Beckton-Dickinson) gel tubes followed by extraction of steroids from serum, reconstitution with mobile phase, and subsequent analysis of various steroids using LC-MS/MS in a single run.</p><p><strong>Results: </strong>No interfering peak was observed when blood was collected in plain red top tube for all five steroids. However, we observed clinically significant interference with low testosterone values when blood specimens were collected in BD gel tubes. For DHEA, we observed interference when blood was collected in BD gel tubes and Griner tubes. Other steroids were not affected.</p><p><strong>Conclusions: </strong>We concluded that BD serum separator gel tube may not be suitable for analysis of testosterone in women and DHEA for both men and women.</p>\",\"PeriodicalId\":8228,\"journal\":{\"name\":\"Annals of clinical and laboratory science\",\"volume\":\"55 3\",\"pages\":\"449-452\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of clinical and laboratory science\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of clinical and laboratory science","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Technical Note: Comparison of Plain and Serum-Separator Blood Collection Tubes for the Measurement of Steroid Hormones by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS): Observation of Erroneous Peaks in Serum Separator Tubes for Analysis of Testosterone and Dehydroepiandrosterone.
Objective: Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) is the gold standard for the analysis of steroids including 11-deoxycortisol, androstenedione, testosterone, 17-hydroxyprogesterone, and dehydroepiandrosterone (DHEA) in serum or plasma. In our hospital we observed an occasional problem in analysis of testosterone in some patients when blood was collected in Beckton-Dickinson serum separator gel tubes (BD gel tubes). Therefore, we investigated this issue.
Methods: Blood was collected from 10 volunteers (five males and five females) simultaneously in plain red top tubes (no gel), Greiner serum separator gel tubes (Greiner gel tube), and BD (Beckton-Dickinson) gel tubes followed by extraction of steroids from serum, reconstitution with mobile phase, and subsequent analysis of various steroids using LC-MS/MS in a single run.
Results: No interfering peak was observed when blood was collected in plain red top tube for all five steroids. However, we observed clinically significant interference with low testosterone values when blood specimens were collected in BD gel tubes. For DHEA, we observed interference when blood was collected in BD gel tubes and Griner tubes. Other steroids were not affected.
Conclusions: We concluded that BD serum separator gel tube may not be suitable for analysis of testosterone in women and DHEA for both men and women.
期刊介绍:
The Annals of Clinical & Laboratory Science
welcomes manuscripts that report research in clinical
science, including pathology, clinical chemistry,
biotechnology, molecular biology, cytogenetics,
microbiology, immunology, hematology, transfusion
medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.