Wenqing Hong , Shu Yang , Weixi Shu , Gordon Price , Liyan Song
{"title":"多领域微生物群相互作用的时空动态驱动垃圾填埋场CNPS循环","authors":"Wenqing Hong , Shu Yang , Weixi Shu , Gordon Price , Liyan Song","doi":"10.1016/j.wasman.2025.115048","DOIUrl":null,"url":null,"abstract":"<div><div>Landfill ecosystems represent significant terrestrial carbon sinks, where municipal solid waste (MSW) undergoes decomposition and transformation through biochemical reactions mediated by multi-kingdom microbiome. However, the spatial and temporal characterization of microbial interactions and collaboration within these multi-kingdom microbiomes remain largely unknown. In this study, we reveal the hierarchical and collaborative mechanisms by which multi-kingdom microbiomes drive carbon, nitrogen, phosphorus, and sulfur cycling across a 30-meter landfilling depth, corresponding to a landfilling age of 1 to 4 years. Through integration of metagenomics and network analyses, we elucidated vertical succession patterns in microbial community structure and function. The surface layer (1–2 years) was centered on bacterial-dominated primary metabolism, with <em>Enterococcus aquimarinus</em> and <em>Brevundimonas bullata</em> inferred to mediate metabolic coupling through fermentation, nitrogen fixation andphosphorus solubilization. Concurrently, phage-driven host lysis may contribute to the suppression of acidification. In the middle layer (2–3 years), co-occurrence patterns between archaea and fungi suggest mutualistic interaction supporting methanogenesis, wherein fungi such as <em>Anaeromyces robustus</em> would supply key substrates for methanogens such as <em>Methanofollis ethanolicus</em>. In the bottom layer (3–4 years), fungi, bacteria, and viruses collaborate under nutrient-limited conditions, with phages employing a “kill-the-winner” approach to sustain ecosystem function and stability. Collectively, our findings indicate that key microbial connectors across kingdoms contribute to elemental cycling through cross-kingdom interaction, including substrate exchange and nutrient supply. This study advances our understanding of multi-kingdom microbial dynamics during MSW decomposition and offers a conceptual framework for enhancing biogeochemical cycling efficiency within landfill ecosystems.</div></div>","PeriodicalId":23969,"journal":{"name":"Waste management","volume":"206 ","pages":"Article 115048"},"PeriodicalIF":7.1000,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Spatiotemporal dynamics of multi-kingdom microbiome interactions drive CNPS cycling in landfills\",\"authors\":\"Wenqing Hong , Shu Yang , Weixi Shu , Gordon Price , Liyan Song\",\"doi\":\"10.1016/j.wasman.2025.115048\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Landfill ecosystems represent significant terrestrial carbon sinks, where municipal solid waste (MSW) undergoes decomposition and transformation through biochemical reactions mediated by multi-kingdom microbiome. However, the spatial and temporal characterization of microbial interactions and collaboration within these multi-kingdom microbiomes remain largely unknown. In this study, we reveal the hierarchical and collaborative mechanisms by which multi-kingdom microbiomes drive carbon, nitrogen, phosphorus, and sulfur cycling across a 30-meter landfilling depth, corresponding to a landfilling age of 1 to 4 years. Through integration of metagenomics and network analyses, we elucidated vertical succession patterns in microbial community structure and function. The surface layer (1–2 years) was centered on bacterial-dominated primary metabolism, with <em>Enterococcus aquimarinus</em> and <em>Brevundimonas bullata</em> inferred to mediate metabolic coupling through fermentation, nitrogen fixation andphosphorus solubilization. Concurrently, phage-driven host lysis may contribute to the suppression of acidification. In the middle layer (2–3 years), co-occurrence patterns between archaea and fungi suggest mutualistic interaction supporting methanogenesis, wherein fungi such as <em>Anaeromyces robustus</em> would supply key substrates for methanogens such as <em>Methanofollis ethanolicus</em>. In the bottom layer (3–4 years), fungi, bacteria, and viruses collaborate under nutrient-limited conditions, with phages employing a “kill-the-winner” approach to sustain ecosystem function and stability. Collectively, our findings indicate that key microbial connectors across kingdoms contribute to elemental cycling through cross-kingdom interaction, including substrate exchange and nutrient supply. This study advances our understanding of multi-kingdom microbial dynamics during MSW decomposition and offers a conceptual framework for enhancing biogeochemical cycling efficiency within landfill ecosystems.</div></div>\",\"PeriodicalId\":23969,\"journal\":{\"name\":\"Waste management\",\"volume\":\"206 \",\"pages\":\"Article 115048\"},\"PeriodicalIF\":7.1000,\"publicationDate\":\"2025-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Waste management\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0956053X25004593\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Waste management","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956053X25004593","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Spatiotemporal dynamics of multi-kingdom microbiome interactions drive CNPS cycling in landfills
Landfill ecosystems represent significant terrestrial carbon sinks, where municipal solid waste (MSW) undergoes decomposition and transformation through biochemical reactions mediated by multi-kingdom microbiome. However, the spatial and temporal characterization of microbial interactions and collaboration within these multi-kingdom microbiomes remain largely unknown. In this study, we reveal the hierarchical and collaborative mechanisms by which multi-kingdom microbiomes drive carbon, nitrogen, phosphorus, and sulfur cycling across a 30-meter landfilling depth, corresponding to a landfilling age of 1 to 4 years. Through integration of metagenomics and network analyses, we elucidated vertical succession patterns in microbial community structure and function. The surface layer (1–2 years) was centered on bacterial-dominated primary metabolism, with Enterococcus aquimarinus and Brevundimonas bullata inferred to mediate metabolic coupling through fermentation, nitrogen fixation andphosphorus solubilization. Concurrently, phage-driven host lysis may contribute to the suppression of acidification. In the middle layer (2–3 years), co-occurrence patterns between archaea and fungi suggest mutualistic interaction supporting methanogenesis, wherein fungi such as Anaeromyces robustus would supply key substrates for methanogens such as Methanofollis ethanolicus. In the bottom layer (3–4 years), fungi, bacteria, and viruses collaborate under nutrient-limited conditions, with phages employing a “kill-the-winner” approach to sustain ecosystem function and stability. Collectively, our findings indicate that key microbial connectors across kingdoms contribute to elemental cycling through cross-kingdom interaction, including substrate exchange and nutrient supply. This study advances our understanding of multi-kingdom microbial dynamics during MSW decomposition and offers a conceptual framework for enhancing biogeochemical cycling efficiency within landfill ecosystems.
期刊介绍:
Waste Management is devoted to the presentation and discussion of information on solid wastes,it covers the entire lifecycle of solid. wastes.
Scope:
Addresses solid wastes in both industrialized and economically developing countries
Covers various types of solid wastes, including:
Municipal (e.g., residential, institutional, commercial, light industrial)
Agricultural
Special (e.g., C and D, healthcare, household hazardous wastes, sewage sludge)