基于第一代生物传感器的试纸，具有扩展的线性范围，可以在全血中检测葡萄糖

IF 5.6 3区材料科学 Q1 ELECTROCHEMISTRY

Electrochimica Acta Pub Date : 2025-08-02 DOI:10.1016/j.electacta.2025.147060

Maria A. Komkova , Artem A. Eliseev , Andrei A. Poyarkov , Darya V. Vokhmyanina , Andrei A. Eliseev , Arkady A. Karyakin

{"title":"基于第一代生物传感器的试纸，具有扩展的线性范围，可以在全血中检测葡萄糖","authors":"Maria A. Komkova , Artem A. Eliseev , Andrei A. Poyarkov , Darya V. Vokhmyanina , Andrei A. Eliseev , Arkady A. Karyakin","doi":"10.1016/j.electacta.2025.147060","DOIUrl":null,"url":null,"abstract":"<div><div>We report on the approach enabling first-generation biosensor-based test-strips to respond linearly up to 40 mM glucose concentration. Glucose oxidase (GOD) has been chosen as the most selective biocatalyst. Decreasing its content in the enzyme containing membrane so that [E]<sub>0</sub> ≈ [S]<sub>0</sub>, it is possible to increase an apparent Michaelis constant prolonging linear calibration range. Prussian Blue (PB) acting as a transducer practically prevents false-positive responses caused by reductants. Novel bi-pulse power generation readout with overall measurement duration of 5.005 s provides up to 10-fold enhanced sensitivity (17 ± 3 mA·M<sup>−1</sup>·cm<sup>−2</sup>), which compensates its obvious loss caused by the decrease of the enzyme content. Coupled with an advanced readout, the resulting test-strips become suitable for whole blood analysis even upon monitoring of diabetes.</div></div>","PeriodicalId":305,"journal":{"name":"Electrochimica Acta","volume":"538 ","pages":"Article 147060"},"PeriodicalIF":5.6000,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Test-strips based on the first-generation biosensors with expanded linear range enabling glucose detection in whole blood\",\"authors\":\"Maria A. Komkova , Artem A. Eliseev , Andrei A. Poyarkov , Darya V. Vokhmyanina , Andrei A. Eliseev , Arkady A. Karyakin\",\"doi\":\"10.1016/j.electacta.2025.147060\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>We report on the approach enabling first-generation biosensor-based test-strips to respond linearly up to 40 mM glucose concentration. Glucose oxidase (GOD) has been chosen as the most selective biocatalyst. Decreasing its content in the enzyme containing membrane so that [E]<sub>0</sub> ≈ [S]<sub>0</sub>, it is possible to increase an apparent Michaelis constant prolonging linear calibration range. Prussian Blue (PB) acting as a transducer practically prevents false-positive responses caused by reductants. Novel bi-pulse power generation readout with overall measurement duration of 5.005 s provides up to 10-fold enhanced sensitivity (17 ± 3 mA·M<sup>−1</sup>·cm<sup>−2</sup>), which compensates its obvious loss caused by the decrease of the enzyme content. Coupled with an advanced readout, the resulting test-strips become suitable for whole blood analysis even upon monitoring of diabetes.</div></div>\",\"PeriodicalId\":305,\"journal\":{\"name\":\"Electrochimica Acta\",\"volume\":\"538 \",\"pages\":\"Article 147060\"},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2025-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Electrochimica Acta\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0013468625014203\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ELECTROCHEMISTRY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electrochimica Acta","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0013468625014203","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ELECTROCHEMISTRY","Score":null,"Total":0}

引用次数: 0

摘要

我们报告了使第一代基于生物传感器的试纸能够线性响应高达40 mM葡萄糖浓度的方法。葡萄糖氧化酶（GOD）被认为是最具选择性的生物催化剂。降低其在含酶膜中的含量，使[E]0≈[S]0，可以增加表观米切里斯常数，延长线性校准范围。普鲁士蓝（PB）作为换能器实际上可以防止由还原剂引起的假阳性反应。新型双脉冲发电读数，总测量持续时间为5.005 s，灵敏度提高了10倍（17±3 mA·M−1·cm−2），弥补了酶含量下降造成的明显损失。再加上先进的读数，由此产生的试纸甚至可以用于监测糖尿病的全血分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Test-strips based on the first-generation biosensors with expanded linear range enabling glucose detection in whole blood

查看原文本刊更多论文

Test-strips based on the first-generation biosensors with expanded linear range enabling glucose detection in whole blood

We report on the approach enabling first-generation biosensor-based test-strips to respond linearly up to 40 mM glucose concentration. Glucose oxidase (GOD) has been chosen as the most selective biocatalyst. Decreasing its content in the enzyme containing membrane so that [E]₀ ≈ [S]₀, it is possible to increase an apparent Michaelis constant prolonging linear calibration range. Prussian Blue (PB) acting as a transducer practically prevents false-positive responses caused by reductants. Novel bi-pulse power generation readout with overall measurement duration of 5.005 s provides up to 10-fold enhanced sensitivity (17 ± 3 mA·M⁻¹·cm⁻²), which compensates its obvious loss caused by the decrease of the enzyme content. Coupled with an advanced readout, the resulting test-strips become suitable for whole blood analysis even upon monitoring of diabetes.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Electrochimica Acta 工程技术-电化学

CiteScore

11.30

自引率

6.10%

发文量

1634

审稿时长

41 days

期刊介绍： Electrochimica Acta is an international journal. It is intended for the publication of both original work and reviews in the field of electrochemistry. Electrochemistry should be interpreted to mean any of the research fields covered by the Divisions of the International Society of Electrochemistry listed below, as well as emerging scientific domains covered by ISE New Topics Committee.