{"title":"携带环状小染色体的拟南芥系谱的不育性和结构变异。","authors":"Benny Ordoñez, Weier Guo, Witsarut Chueakhunthod, Isabelle M Henry, Luca Comai","doi":"10.1007/s10577-025-09776-0","DOIUrl":null,"url":null,"abstract":"<p><p>Circular minichromosomes could be useful tools for plant biotechnology, yet their long-term structural stability, heritability, and effects on phenotype remain poorly understood. In this study, we report a multi-generational analysis of the Arabidopsis mini1a ring minichromosome, which originated from the chromosome 1 centromere in a haploid induction cross. Is mini1a unstable, as suggested by classical studies on other ring chromosomes? Using whole-genome sequencing of individuals carrying mini1a representing multiple successive generations, we uncovered a major catastrophe driven by DNA breaks and novel junction formation, resulting in a new version of mini1a, that carries a 1.3 Mb deletion in the centromeric region (mini1aΔ). We identified 20 new breakpoints, of which 7 disrupted gene bodies-a frequency unlikely to occur by chance. Interestingly, both mini1a and mini1aΔ could exist in one or two copies and could co-exist in a single plant. Although they were inherited efficiently, their presence was sometimes associated with plant sectors with 100% sterility. These findings highlight the structural plasticity of mini1a. At the same time, they raise questions regarding the mechanisms underlying the observed reduced plant fertility. In summary, circular minichromosomes can be deleterious and biotechnology applications based on the manipulation of minichromosomes will require careful planning and testing.</p>","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"33 1","pages":"16"},"PeriodicalIF":2.8000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12316722/pdf/","citationCount":"0","resultStr":"{\"title\":\"Sterility and structural variation in an arabidopsis pedigree carrying a ring minichromosome.\",\"authors\":\"Benny Ordoñez, Weier Guo, Witsarut Chueakhunthod, Isabelle M Henry, Luca Comai\",\"doi\":\"10.1007/s10577-025-09776-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Circular minichromosomes could be useful tools for plant biotechnology, yet their long-term structural stability, heritability, and effects on phenotype remain poorly understood. In this study, we report a multi-generational analysis of the Arabidopsis mini1a ring minichromosome, which originated from the chromosome 1 centromere in a haploid induction cross. Is mini1a unstable, as suggested by classical studies on other ring chromosomes? Using whole-genome sequencing of individuals carrying mini1a representing multiple successive generations, we uncovered a major catastrophe driven by DNA breaks and novel junction formation, resulting in a new version of mini1a, that carries a 1.3 Mb deletion in the centromeric region (mini1aΔ). We identified 20 new breakpoints, of which 7 disrupted gene bodies-a frequency unlikely to occur by chance. Interestingly, both mini1a and mini1aΔ could exist in one or two copies and could co-exist in a single plant. Although they were inherited efficiently, their presence was sometimes associated with plant sectors with 100% sterility. These findings highlight the structural plasticity of mini1a. At the same time, they raise questions regarding the mechanisms underlying the observed reduced plant fertility. In summary, circular minichromosomes can be deleterious and biotechnology applications based on the manipulation of minichromosomes will require careful planning and testing.</p>\",\"PeriodicalId\":50698,\"journal\":{\"name\":\"Chromosome Research\",\"volume\":\"33 1\",\"pages\":\"16\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12316722/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chromosome Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10577-025-09776-0\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromosome Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10577-025-09776-0","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Sterility and structural variation in an arabidopsis pedigree carrying a ring minichromosome.
Circular minichromosomes could be useful tools for plant biotechnology, yet their long-term structural stability, heritability, and effects on phenotype remain poorly understood. In this study, we report a multi-generational analysis of the Arabidopsis mini1a ring minichromosome, which originated from the chromosome 1 centromere in a haploid induction cross. Is mini1a unstable, as suggested by classical studies on other ring chromosomes? Using whole-genome sequencing of individuals carrying mini1a representing multiple successive generations, we uncovered a major catastrophe driven by DNA breaks and novel junction formation, resulting in a new version of mini1a, that carries a 1.3 Mb deletion in the centromeric region (mini1aΔ). We identified 20 new breakpoints, of which 7 disrupted gene bodies-a frequency unlikely to occur by chance. Interestingly, both mini1a and mini1aΔ could exist in one or two copies and could co-exist in a single plant. Although they were inherited efficiently, their presence was sometimes associated with plant sectors with 100% sterility. These findings highlight the structural plasticity of mini1a. At the same time, they raise questions regarding the mechanisms underlying the observed reduced plant fertility. In summary, circular minichromosomes can be deleterious and biotechnology applications based on the manipulation of minichromosomes will require careful planning and testing.
期刊介绍:
Chromosome Research publishes manuscripts from work based on all organisms and encourages submissions in the following areas including, but not limited, to:
· Chromosomes and their linkage to diseases;
· Chromosome organization within the nucleus;
· Chromatin biology (transcription, non-coding RNA, etc);
· Chromosome structure, function and mechanics;
· Chromosome and DNA repair;
· Epigenetic chromosomal functions (centromeres, telomeres, replication, imprinting,
dosage compensation, sex determination, chromosome remodeling);
· Architectural/epigenomic organization of the genome;
· Functional annotation of the genome;
· Functional and comparative genomics in plants and animals;
· Karyology studies that help resolve difficult taxonomic problems or that provide
clues to fundamental mechanisms of genome and karyotype evolution in plants and animals;
· Mitosis and Meiosis;
· Cancer cytogenomics.