携带环状小染色体的拟南芥系谱的不育性和结构变异。

IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Benny Ordoñez, Weier Guo, Witsarut Chueakhunthod, Isabelle M Henry, Luca Comai
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引用次数: 0

摘要

环状小染色体可能是植物生物技术的有用工具,但其长期结构稳定性、遗传力和对表型的影响尚不清楚。在这项研究中,我们报道了对拟南芥mini1a环状小染色体的多代分析,该染色体起源于单倍体诱导杂交中的1号染色体着丝粒。mini1a是不稳定的,如经典研究建议的其他环染色体?通过对多代携带mini1a的个体进行全基因组测序,我们发现了由DNA断裂和新连接形成驱动的重大灾难,导致新版本的mini1a,在着丝粒区携带1.3 Mb的缺失(mini1aΔ)。我们发现了20个新的断点,其中7个基因体被破坏——这种频率不太可能偶然发生。有趣的是,mini1a和mini1aΔ都可以存在于一个或两个副本中,并且可以在单个植物中共存。虽然它们是有效遗传的,但它们的存在有时与100%不育的植物部门有关。这些发现突出了mini1a的结构可塑性。同时,他们对观察到的植物肥力下降的机制提出了疑问。总之,圆形小染色体可能是有害的,基于小染色体操作的生物技术应用将需要仔细的计划和测试。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Sterility and structural variation in an arabidopsis pedigree carrying a ring minichromosome.

Sterility and structural variation in an arabidopsis pedigree carrying a ring minichromosome.

Sterility and structural variation in an arabidopsis pedigree carrying a ring minichromosome.

Sterility and structural variation in an arabidopsis pedigree carrying a ring minichromosome.

Circular minichromosomes could be useful tools for plant biotechnology, yet their long-term structural stability, heritability, and effects on phenotype remain poorly understood. In this study, we report a multi-generational analysis of the Arabidopsis mini1a ring minichromosome, which originated from the chromosome 1 centromere in a haploid induction cross. Is mini1a unstable, as suggested by classical studies on other ring chromosomes? Using whole-genome sequencing of individuals carrying mini1a representing multiple successive generations, we uncovered a major catastrophe driven by DNA breaks and novel junction formation, resulting in a new version of mini1a, that carries a 1.3 Mb deletion in the centromeric region (mini1aΔ). We identified 20 new breakpoints, of which 7 disrupted gene bodies-a frequency unlikely to occur by chance. Interestingly, both mini1a and mini1aΔ could exist in one or two copies and could co-exist in a single plant. Although they were inherited efficiently, their presence was sometimes associated with plant sectors with 100% sterility. These findings highlight the structural plasticity of mini1a. At the same time, they raise questions regarding the mechanisms underlying the observed reduced plant fertility. In summary, circular minichromosomes can be deleterious and biotechnology applications based on the manipulation of minichromosomes will require careful planning and testing.

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来源期刊
Chromosome Research
Chromosome Research 生物-生化与分子生物学
CiteScore
4.70
自引率
3.80%
发文量
31
审稿时长
1 months
期刊介绍: Chromosome Research publishes manuscripts from work based on all organisms and encourages submissions in the following areas including, but not limited, to: · Chromosomes and their linkage to diseases; · Chromosome organization within the nucleus; · Chromatin biology (transcription, non-coding RNA, etc); · Chromosome structure, function and mechanics; · Chromosome and DNA repair; · Epigenetic chromosomal functions (centromeres, telomeres, replication, imprinting, dosage compensation, sex determination, chromosome remodeling); · Architectural/epigenomic organization of the genome; · Functional annotation of the genome; · Functional and comparative genomics in plants and animals; · Karyology studies that help resolve difficult taxonomic problems or that provide clues to fundamental mechanisms of genome and karyotype evolution in plants and animals; · Mitosis and Meiosis; · Cancer cytogenomics.
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