Guangbin Luo, Mai Duy Luu Trinh, Margrethe Kristine Dam Falkenberg, Maurizio Junior Chiurazzi, Javad Najafi, Anton Frisgaard Nørrevang, Pedro Miguel Pereira Correia, Michael Palmgren
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Unlocking in vitro transformation of recalcitrant plants.
Genome editing offers powerful opportunities for crop improvement by enabling precise and targeted mutagenesis. Tools such as CRISPR-associated protein 9 and single-guide RNAs can be introduced into plant cells via in vitro transformation, which, despite the rise of in planta methods, remains an important method because it is highly effective when successful. However, transformation-induced stress is a critical and underexplored barrier to successful in vitro transformation, especially in recalcitrant plants. In this opinion article, we discuss in vitro methods for transforming recalcitrant plants, the challenges encountered, and potential solutions through the conceptual lens of stress biology. Reducing cellular stress, transiently weakening the immune response and optimizing regeneration protocols may be essential for expanding the transformation capacity across a broader range of plant species.
期刊介绍：
Trends in Plant Science is the primary monthly review journal in plant science, encompassing a wide range from molecular biology to ecology. It offers concise and accessible reviews and opinions on fundamental plant science topics, providing quick insights into current thinking and developments in plant biology. Geared towards researchers, students, and teachers, the articles are authoritative, authored by both established leaders in the field and emerging talents.
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