揭示跨喜马拉雅西藏河马低温和霜冻胁迫响应的转录调控机制。

IF 3.8 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Romit Seth, Amna Devi, Phuntsog Dolkar, Rajni Parmar, Shikha Sharma, Balraj Sharma, Praveen Dhyani, Tsering Stobdan, Ram Kumar Sharma
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引用次数: 0

摘要

西藏河马是一种神秘的、最少被探索的沙棘物种,它对跨喜马拉雅地区的零下温度具有特殊的适应性。本研究整合了生理和转录分析来了解其独特的冷胁迫恢复能力。与延长响应(PR)和冻结响应(FR)相比,冷胁迫早期响应(ER)对叶绿素含量、相对含水量和电解质泄漏等生理指标的影响最小,而延长响应(PR)和冻结响应(FR)在恢复阶段(RR)得到有效恢复。基因组引导的从头组装得到25176个高质量的单基因(N50: 2195 bp;BUSCO: 92.9%),其中75.9%使用NCBI-nr、arport11、SwissProt、COG、KEGG和Pfam数据库进行功能注释。差异表达的单基因聚类显示,ER (4467 DEGs)和RR (4478 DEGs)与PR (14,150 DEGs)和FR (14,528 DEGs)明显不同,这表明PR/FR期间的转录重编程明显高于ER/RR。此外,转录相互作用网络与GO和KEGG富集的整合突出了ICE1-CBF调控网络,CBF表达诱导剂(ICE1)、冷接受蛋白激酶(CRPK1)、抗冻蛋白(AFPs)以及诸如jasmonic酸信号通路、碳水化合物代谢和膜稳定等途径在PR和FR阶段是耐寒性的关键。本研究进一步加深了我们对西藏人冷应激恢复能力的认识,阐明了西藏人在跨喜马拉雅极端环境下的适应机制。本文确定的综合基因组资源和关键候选基因可能为在重点作物和人工林物种中发现耐寒性相关的全基因组变异提供基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unveiling transcriptional regulation underpinning chilling and frost stress response in Trans-Himalayan Hippophae tibetana L.

Hippophae tibetana is an enigmatic least explored Seabuckthorn species, with exceptional adaptability to sub-zero temperatures in Trans-Himalayan region. This study integrates physiological and transcriptional profiling to understand its unique cold stress resilience. The physiological assessment including chlorophyll content, relative water content, and electrolyte leakage were least affected during the early response (ER) of cold stress as compared to prolonged (PR) and freeze response (FR), which was effectively restored during the recovery phase (RR). Genome-guided de novo assembly yielded 25,176 high-quality unigenes (N50: 2195 bp; BUSCO: 92.9%), with 75.9% functionally annotated using NCBI-nr, Araport11, SwissProt, COG, KEGG, and Pfam databases. Clustering of differentially expressed unigenes revealed ER (4467 DEGs) and RR (4478) grouped distinctly from PR (14,150) and FR (14,528), underscoring significantly heightened transcriptional reprogramming during PR/FR compared to ER/RR. Furthermore, the integration of transcriptional interactome network with GO and KEGG enrichment highlighted ICE1-CBF regulatory network with significant upregulation of Inducer of CBF Expression (ICE1), Cold receptive protein kinase (CRPK1), anti-freeze proteins (AFPs), and pathways like jasmonic acid signaling, carbohydrate metabolism, and membrane stabilization as key to cold tolerance during PR and FR phases. The current study advances our understanding of cold stress resilience in H. tibetana, elucidating its adaptive mechanisms in extreme Trans-Himalayan environments. The comprehensive genomic resources and key candidates identified here may provide a foundation for discovering cold tolerance-associated genome-wide variations in priority crops and plantation species.

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来源期刊
Plant Molecular Biology
Plant Molecular Biology 生物-生化与分子生物学
自引率
2.00%
发文量
95
审稿时长
1.4 months
期刊介绍: Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.
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