{"title":"在伊朗德黑兰选定的医疗中心使用血清学和分子技术评估慢性肾脏疾病(CKD)患者中弓形虫和弓形虫的患病率。","authors":"Bahare Razmand, Farid Tahvildar Biderouni, Alireza Abadi, Niloofar Taghipour","doi":"10.18502/ijpa.v20i2.19028","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Toxoplasmosis and toxocariasis are two zoonotic diseases with global impact. Chronic kidney disease (CKD) can lead to complications, associated with reduced immune responses that predispose them to frequent parasitic infections. We aimed to determine the prevalence of toxoplasmosis and toxocariasis in CKD patients to propose a new way to control them in dialyses duration in Tehran, Iran.</p><p><strong>Methods: </strong>Three hundred and sixty-five CKD patients and 72 healthy individuals were tested for anti-<i>Toxoplasma gondii</i> (IgG, IgM) and anti-<i>Toxocara</i> (IgG) antibodies using conventional ELISA technique. IgM positive samples underwent genetic analysis.</p><p><strong>Results: </strong>Of the 437 samples studied (365 patients and 72 controls), 182 CKD patients (49.8%) and 20 controls (27.9%) were positive for <i>Toxoplasma</i> IgG, and 8 (2.2%) CKD samples were positive for <i>Toxoplasma</i> IgM, while none of the control samples were positive for <i>Toxoplasma</i> IgM. Molecular analysis of the 8 IgM positive samples with B1gene confirmed the presence of Toxoplasma antigen in all of them. <i>Toxocara</i> IgG antibodies showed a lower prevalence in CKD patients (5.7%) compared to controls (9.7%). The study revealed significant differences (<i>P</i> <0.05) in <i>Toxoplasma</i> IgG and IgM antibodies, as well as variables such as dialysis and dialysis duration between the two groups of patients and the control group. However, variables such as <i>Toxocara</i> IgG, age, gender, lupus, and pets showed no significant difference between the control group and the patients.</p><p><strong>Conclusion: </strong>The collected data in this study could serve as a reference for future studies and may be useful for examination and evaluation of acute and chronic toxoplasmosis before and after starting dialysis.</p>","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"20 2","pages":"227-235"},"PeriodicalIF":0.9000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12307789/pdf/","citationCount":"0","resultStr":"{\"title\":\"Evaluation of <i>Toxoplasma</i> and <i>Toxocara</i> Prevalence in Chronic Kidney Disease (CKD) Patients Using Serologic and Molecular Technique in Selected Medical Centers of Tehran, Iran.\",\"authors\":\"Bahare Razmand, Farid Tahvildar Biderouni, Alireza Abadi, Niloofar Taghipour\",\"doi\":\"10.18502/ijpa.v20i2.19028\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Toxoplasmosis and toxocariasis are two zoonotic diseases with global impact. Chronic kidney disease (CKD) can lead to complications, associated with reduced immune responses that predispose them to frequent parasitic infections. We aimed to determine the prevalence of toxoplasmosis and toxocariasis in CKD patients to propose a new way to control them in dialyses duration in Tehran, Iran.</p><p><strong>Methods: </strong>Three hundred and sixty-five CKD patients and 72 healthy individuals were tested for anti-<i>Toxoplasma gondii</i> (IgG, IgM) and anti-<i>Toxocara</i> (IgG) antibodies using conventional ELISA technique. IgM positive samples underwent genetic analysis.</p><p><strong>Results: </strong>Of the 437 samples studied (365 patients and 72 controls), 182 CKD patients (49.8%) and 20 controls (27.9%) were positive for <i>Toxoplasma</i> IgG, and 8 (2.2%) CKD samples were positive for <i>Toxoplasma</i> IgM, while none of the control samples were positive for <i>Toxoplasma</i> IgM. Molecular analysis of the 8 IgM positive samples with B1gene confirmed the presence of Toxoplasma antigen in all of them. <i>Toxocara</i> IgG antibodies showed a lower prevalence in CKD patients (5.7%) compared to controls (9.7%). The study revealed significant differences (<i>P</i> <0.05) in <i>Toxoplasma</i> IgG and IgM antibodies, as well as variables such as dialysis and dialysis duration between the two groups of patients and the control group. However, variables such as <i>Toxocara</i> IgG, age, gender, lupus, and pets showed no significant difference between the control group and the patients.</p><p><strong>Conclusion: </strong>The collected data in this study could serve as a reference for future studies and may be useful for examination and evaluation of acute and chronic toxoplasmosis before and after starting dialysis.</p>\",\"PeriodicalId\":14669,\"journal\":{\"name\":\"Iranian Journal of Parasitology\",\"volume\":\"20 2\",\"pages\":\"227-235\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12307789/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/ijpa.v20i2.19028\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/ijpa.v20i2.19028","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Evaluation of Toxoplasma and Toxocara Prevalence in Chronic Kidney Disease (CKD) Patients Using Serologic and Molecular Technique in Selected Medical Centers of Tehran, Iran.
Background: Toxoplasmosis and toxocariasis are two zoonotic diseases with global impact. Chronic kidney disease (CKD) can lead to complications, associated with reduced immune responses that predispose them to frequent parasitic infections. We aimed to determine the prevalence of toxoplasmosis and toxocariasis in CKD patients to propose a new way to control them in dialyses duration in Tehran, Iran.
Methods: Three hundred and sixty-five CKD patients and 72 healthy individuals were tested for anti-Toxoplasma gondii (IgG, IgM) and anti-Toxocara (IgG) antibodies using conventional ELISA technique. IgM positive samples underwent genetic analysis.
Results: Of the 437 samples studied (365 patients and 72 controls), 182 CKD patients (49.8%) and 20 controls (27.9%) were positive for Toxoplasma IgG, and 8 (2.2%) CKD samples were positive for Toxoplasma IgM, while none of the control samples were positive for Toxoplasma IgM. Molecular analysis of the 8 IgM positive samples with B1gene confirmed the presence of Toxoplasma antigen in all of them. Toxocara IgG antibodies showed a lower prevalence in CKD patients (5.7%) compared to controls (9.7%). The study revealed significant differences (P <0.05) in Toxoplasma IgG and IgM antibodies, as well as variables such as dialysis and dialysis duration between the two groups of patients and the control group. However, variables such as Toxocara IgG, age, gender, lupus, and pets showed no significant difference between the control group and the patients.
Conclusion: The collected data in this study could serve as a reference for future studies and may be useful for examination and evaluation of acute and chronic toxoplasmosis before and after starting dialysis.
期刊介绍:
Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members.
The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.