{"title":"伊朗Semnan地区家猫中猫科细胞繁殖体的实时荧光定量PCR分子研究。","authors":"Masoud Ebrahimi Toodarvari, Hesamedin Eskafian, Hamid Staji, Mahmood Ahmadi-Hamedani","doi":"10.18502/ijpa.v20i2.19033","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Cytauxzoon felis</i> is a protozoan parasite transmitted by ticks and affects felids. Acute infection in domestic cats is characterized by symptoms such as lethargy, anorexia, fever, and anemia.</p><p><strong>Methods: </strong>The present study focuses on diagnosing and molecularly identifying <i>C. felis</i> using a real-time PCR method in cats from Semnan, Iran. During the winter and spring of 2024, two hundred cats were randomly selected from veterinary clinics in Semnan. Blood samples were collected from the cats for DNA extraction and molecular analysis. Samples were divided into 40 pooled of 5 samples, each consisting of a combination of 5 blood samples. Then, the genomic DNAs were extracted from blood specimens and screened by real-time PCR (RT-PCR) for the presence of <i>C. felis</i> infection by amplifying of ITS2 gene belonging to the <i>Cytauxzoon</i> genus.</p><p><strong>Results: </strong>The results indicated that 6 out of the 200 blood samples were infected (3%).</p><p><strong>Conclusion: </strong>This study was conducted for the first time in Semnan and shows that the prevalence of <i>C. felis</i> in cats is significant.</p>","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"20 2","pages":"261-267"},"PeriodicalIF":0.9000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12307781/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>Cytauxzoon felis</i> in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, Iran.\",\"authors\":\"Masoud Ebrahimi Toodarvari, Hesamedin Eskafian, Hamid Staji, Mahmood Ahmadi-Hamedani\",\"doi\":\"10.18502/ijpa.v20i2.19033\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong><i>Cytauxzoon felis</i> is a protozoan parasite transmitted by ticks and affects felids. Acute infection in domestic cats is characterized by symptoms such as lethargy, anorexia, fever, and anemia.</p><p><strong>Methods: </strong>The present study focuses on diagnosing and molecularly identifying <i>C. felis</i> using a real-time PCR method in cats from Semnan, Iran. During the winter and spring of 2024, two hundred cats were randomly selected from veterinary clinics in Semnan. Blood samples were collected from the cats for DNA extraction and molecular analysis. Samples were divided into 40 pooled of 5 samples, each consisting of a combination of 5 blood samples. Then, the genomic DNAs were extracted from blood specimens and screened by real-time PCR (RT-PCR) for the presence of <i>C. felis</i> infection by amplifying of ITS2 gene belonging to the <i>Cytauxzoon</i> genus.</p><p><strong>Results: </strong>The results indicated that 6 out of the 200 blood samples were infected (3%).</p><p><strong>Conclusion: </strong>This study was conducted for the first time in Semnan and shows that the prevalence of <i>C. felis</i> in cats is significant.</p>\",\"PeriodicalId\":14669,\"journal\":{\"name\":\"Iranian Journal of Parasitology\",\"volume\":\"20 2\",\"pages\":\"261-267\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12307781/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/ijpa.v20i2.19033\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/ijpa.v20i2.19033","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Cytauxzoon felis in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, Iran.
Background: Cytauxzoon felis is a protozoan parasite transmitted by ticks and affects felids. Acute infection in domestic cats is characterized by symptoms such as lethargy, anorexia, fever, and anemia.
Methods: The present study focuses on diagnosing and molecularly identifying C. felis using a real-time PCR method in cats from Semnan, Iran. During the winter and spring of 2024, two hundred cats were randomly selected from veterinary clinics in Semnan. Blood samples were collected from the cats for DNA extraction and molecular analysis. Samples were divided into 40 pooled of 5 samples, each consisting of a combination of 5 blood samples. Then, the genomic DNAs were extracted from blood specimens and screened by real-time PCR (RT-PCR) for the presence of C. felis infection by amplifying of ITS2 gene belonging to the Cytauxzoon genus.
Results: The results indicated that 6 out of the 200 blood samples were infected (3%).
Conclusion: This study was conducted for the first time in Semnan and shows that the prevalence of C. felis in cats is significant.
期刊介绍：
Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members.
The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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