Polygonatum sibiricum polysaccharides combat fatty liver haemorrhage syndrome in hens via regulation of lipid metabolism, oxidative stress and inflammatory response.

1. A total of 90 healthy Hyline brown laying hens (44 weeks old) were randomly assigned into three groups, a group of hens with fatty liver haemorrhagic syndrome (FLHS) fed with high-energy low-protein diet (HELPD), which exacerbates the syndrome, and the other two groups fed with HELPD containing 400 and 800 mg/kg PSP, respectively, to investigate the therapeutic effects and underlying mechanisms of Polygonatum sibiricum polysaccharides (PSP) on FLHS.2. Dietary PSP supplementation significantly elevated laying rate of FLHS hens. The FLHS hens fed with PSP displayed significant decrease in ALT, AST, TG, TC and LDL-C, while the increase in ALB, TP and HDL-C compared with FLHS hens.3. Hens receiving PSP had significantly reduced levels of MDA but increased activity of T-AOC, SOD, CAT and GSH-Px in both the serum and liver of hens with FLHS. It inhibited inflammatory response in FLHS hens, as evidenced by reduced levels and expression of IL-1β, TNF-α and IL-6, but elevated levels and expression of IL-10 in the serum and liver.4. Transcriptome analysis confirmed that PSP improved FLHS in laying hens through regulating lipid metabolism (such as PPAR signalling pathway, fatty acid metabolism and fatty acid biosynthesis), redox balance (including FoxO signalling pathway and peroxisome) and inflammation (e.g. adipocytokine signalling pathway and cytokine-cytokine receptor interaction).5. This study indicated that dietary PSP supplementation is a practical strategy to prevent and treat FLHS through improving lipid deposition, oxidative stress and inflammation in laying hens.