Thaís Lima Ferreira, Lívia Gurgel do Amaral Valente Sá, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Beatriz Oliveira de Souza, Bruno Coêlho Cavalcanti, Hemerson Iury Ferreira Magalhães, Iri Sandro Pampolha Lima, Amanda Cavalcante Leitão, Manoel Odorico de Moraes, João Batista de Andrade Neto, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva
{"title":"地西泮对耐甲氧西林金黄色葡萄球菌浮游菌和生物膜菌的抑菌活性。","authors":"Thaís Lima Ferreira, Lívia Gurgel do Amaral Valente Sá, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Beatriz Oliveira de Souza, Bruno Coêlho Cavalcanti, Hemerson Iury Ferreira Magalhães, Iri Sandro Pampolha Lima, Amanda Cavalcante Leitão, Manoel Odorico de Moraes, João Batista de Andrade Neto, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva","doi":"10.1080/08927014.2025.2540534","DOIUrl":null,"url":null,"abstract":"<p><p>The aim was to evaluate the antibacterial activity of diazepam against methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) strains of <i>Staphylococcus aureus</i> and its possible mechanism of action. The broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) of diazepam. A checkerboard assay was used to evaluate the interaction of diazepam with different antibiotics. Colorimetric assays with MTT were used to evaluate the effect of diazepam against the biofilms by MSSA and MRSA. Flow cytometry and fluorescence microscopy were used to evaluate the possible mechanism of action of diazepam against MRSA. Diazepam had a MIC of 256 µg/mL. It only had indifferent interactions with the analyzed antibiotics. Diazepam significantly reduced the viability of MSSA and MRSA biofilms. Diazepam caused fragmentation of bacterial DNA and carbonylation of proteins, resulting in reduced cell viability. Therefore, diazepam has <i>in vitro</i> antimicrobial activity against planktonic and biofilm strains of MRSA and MSSA.</p>","PeriodicalId":8898,"journal":{"name":"Biofouling","volume":" ","pages":"846-856"},"PeriodicalIF":2.0000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antibacterial activity of diazepam against planktonic and biofilm strains of methicillin-resistant <i>Staphylococcus aureus</i>.\",\"authors\":\"Thaís Lima Ferreira, Lívia Gurgel do Amaral Valente Sá, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Beatriz Oliveira de Souza, Bruno Coêlho Cavalcanti, Hemerson Iury Ferreira Magalhães, Iri Sandro Pampolha Lima, Amanda Cavalcante Leitão, Manoel Odorico de Moraes, João Batista de Andrade Neto, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva\",\"doi\":\"10.1080/08927014.2025.2540534\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aim was to evaluate the antibacterial activity of diazepam against methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) strains of <i>Staphylococcus aureus</i> and its possible mechanism of action. The broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) of diazepam. A checkerboard assay was used to evaluate the interaction of diazepam with different antibiotics. Colorimetric assays with MTT were used to evaluate the effect of diazepam against the biofilms by MSSA and MRSA. Flow cytometry and fluorescence microscopy were used to evaluate the possible mechanism of action of diazepam against MRSA. Diazepam had a MIC of 256 µg/mL. It only had indifferent interactions with the analyzed antibiotics. Diazepam significantly reduced the viability of MSSA and MRSA biofilms. Diazepam caused fragmentation of bacterial DNA and carbonylation of proteins, resulting in reduced cell viability. Therefore, diazepam has <i>in vitro</i> antimicrobial activity against planktonic and biofilm strains of MRSA and MSSA.</p>\",\"PeriodicalId\":8898,\"journal\":{\"name\":\"Biofouling\",\"volume\":\" \",\"pages\":\"846-856\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biofouling\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/08927014.2025.2540534\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/1 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biofouling","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/08927014.2025.2540534","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/1 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Antibacterial activity of diazepam against planktonic and biofilm strains of methicillin-resistant Staphylococcus aureus.
The aim was to evaluate the antibacterial activity of diazepam against methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) strains of Staphylococcus aureus and its possible mechanism of action. The broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) of diazepam. A checkerboard assay was used to evaluate the interaction of diazepam with different antibiotics. Colorimetric assays with MTT were used to evaluate the effect of diazepam against the biofilms by MSSA and MRSA. Flow cytometry and fluorescence microscopy were used to evaluate the possible mechanism of action of diazepam against MRSA. Diazepam had a MIC of 256 µg/mL. It only had indifferent interactions with the analyzed antibiotics. Diazepam significantly reduced the viability of MSSA and MRSA biofilms. Diazepam caused fragmentation of bacterial DNA and carbonylation of proteins, resulting in reduced cell viability. Therefore, diazepam has in vitro antimicrobial activity against planktonic and biofilm strains of MRSA and MSSA.
期刊介绍：
Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion.
Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context.
Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
