评估牛粪生物炭在富砷土壤中绿豆幼苗应激缓解中的作用。

IF 5.8 3区 环境科学与生态学 0 ENVIRONMENTAL SCIENCES
Seuj Priya Borah, Mariama Begum, Padmasri Ghosh, Puja Saikia, Debojyoti Moulick, Chira Ranjan Bhattacharjee, Arun Jyoti Nath, Sayak Das, Shuvasish Choudhury
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引用次数: 0

摘要

农业生态系统的砷污染是影响作物质量和人类健康的新威胁之一。因此,迫切需要制定可持续战略来减轻砷污染及其对植物的影响。生物炭是一种主要来自废弃生物质的多孔碳质材料,已知可从各种基质(包括受污染的土壤)中隔离有毒元素,被认为是一种可行和可持续的补救和缓解压力的选择。本研究评价了牛粪生物炭在缓解砷酸盐(As5+)诱导的绿豆幼苗胁迫中的潜在作用。As5+胁迫导致植株生长下降,主要表现在根冠伸长和生物量受到抑制。在没有生物炭处理的情况下,施加As5+胁迫,导致生物炭处理的绿豆幼苗根部和茎部过氧化氢(H2O2)和超氧自由基(O2•-)产量显著增加,而H2O2和O2•-含量显著下降(p≤0.01)。与未添加任何生物炭的As5+胁迫相比,经生物炭处理的绿豆幼苗根系和茎部丙二醛(MDA)含量显著(p≤0.01)下降,且活性氧(ROS)过剩现象明显减少。在过氧化氢酶(CAT)和超氧化物歧化酶(SOD)等酶的抗氧化活性方面,生物炭也有影响,在As5+胁迫下,根和地上部过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性显著高于单独胁迫(p≤0.01)。然而,在没有生物炭处理的情况下,生物炭处理对抗坏血酸(AsA)和总谷胱甘肽(GSH)等非酶促抗氧化剂水平的影响与As5+处理相比没有明显改变。SEM-EDX分析还表明,生物炭通过强化Si、Mg、Fe、Cu、Ca和Zn,改善了As5+胁迫下绿豆幼苗根系和地上部的元素组成;同时,也限制了根和地上部对As5+的吸收和积累。生物炭添加量分别为1.5%和2.0% / kg土壤,2.0%的生物炭添加量对缓解绿豆幼苗As5+诱导的胁迫反应更为有效。本研究结果提示,牛粪生物炭可能通过限制As5+积累,强化必需营养元素,维持As5+胁迫引起的营养失衡,调节活性氧的产生,提高抗氧化防御代谢,从而缓解绿豆幼苗As5+诱导的胁迫反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessing the role of cattle manure–derived biochar in stress mitigation of mung bean seedlings growing in arsenate-enriched soil

Arsenic (As) contamination of agroecosystems is one of the most emerging threats affecting crop quality and human health. Thus, there is an insistent need to develop sustainable strategies for mitigating As-contamination and its impact on plants. Biochar, a porous carbonaceous material mainly derived from waste biomass, is known to sequester toxic elements from various substrates, including contaminated soil, and is considered a viable and sustainable option for remediation as well as stress mitigation. The present study evaluated the prospective role of a biochar derived from cattle manure in mitigating arsenate (As5+) induced stress in mung bean seedlings. The As5+ stress imposition caused a decline in plant growth, which was observed in the inhibition of root and shoot elongation and biomass. The imposition of As5+ stress in the absence of biochar amendment resulted in a strong increase in hydrogen peroxide (H2O2) and superoxide radical (O2•−) production, while H2O2 and O2•− contents declined significantly (p ≤ 0.01) in the root and shoot of mung bean seedlings grown in biochar-amended soil during As5+ stress. With a substantial reduction in reactive oxygen species (ROS) overproduction in mung bean seedlings grown in biochar-amended soil, we observed a significant (p ≤ 0.01) decline in malondialdehyde (MDA) content in the root and shoot during As5+ stress under biochar-amended conditions compared to those under As5+ stress in the absence of any biochar amendment. The impact of biochar was also observed in terms of antioxidant activities of enzymes such as catalase (CAT) and superoxide dismutase (SOD), which showed significantly higher (p ≤ 0.01) activities in both root and shoot during As5+ stress compared to those under As5+ stress alone. However, the effect of biochar amendment in alleviating the levels of non-enzymatic antioxidants such as ascorbate (AsA) and total glutathione (GSH) was not markedly altered from those under As5+ stress in the absence of biochar amendment. The SEM–EDX analysis also showed that biochar amendment improved the elemental composition of mung bean seedlings during As5+ stress in both root and shoot by fortifying Si, Mg, Fe, Cu, Ca, and Zn; at the same time, it restricted the uptake and accumulation of As5+ in both root and shoot. Since the biochar was amended at 1.5% and 2.0% per kg of soil, the effect of 2.0% biochar amendment was found to be more effective in alleviating As5+-induced stress responses in mung bean seedlings. Our results suggested a prospective role of cattle manure biochar in alleviating As5+-induced stress responses in mung bean seedlings by restricting As5+ accumulation and fortifying the essential nutrient elements to uphold the nutrient imbalance induced by As5+ stress, as well as modulating ROS production and uplifting antioxidant defense metabolism.

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来源期刊
CiteScore
8.70
自引率
17.20%
发文量
6549
审稿时长
3.8 months
期刊介绍: Environmental Science and Pollution Research (ESPR) serves the international community in all areas of Environmental Science and related subjects with emphasis on chemical compounds. This includes: - Terrestrial Biology and Ecology - Aquatic Biology and Ecology - Atmospheric Chemistry - Environmental Microbiology/Biobased Energy Sources - Phytoremediation and Ecosystem Restoration - Environmental Analyses and Monitoring - Assessment of Risks and Interactions of Pollutants in the Environment - Conservation Biology and Sustainable Agriculture - Impact of Chemicals/Pollutants on Human and Animal Health It reports from a broad interdisciplinary outlook.
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