Jorge Gominho , Ana Lourenço , Helena Patrício , Teresa Quilhó , António V. Marques
{"title":"从解剖学和化学角度发现桉树软木树皮-一个案例研究","authors":"Jorge Gominho , Ana Lourenço , Helena Patrício , Teresa Quilhó , António V. Marques","doi":"10.1016/j.bse.2025.105096","DOIUrl":null,"url":null,"abstract":"<div><div>This paper presents the first scientific investigation of the corky bark of <em>Eucalyptus suberea</em> (cork mallee). The study involved two trees with diameters of 23 cm (A) and 47 cm (B), collected from a plantation in Portugal. Anatomical analysis, performed by scanning electron microscopy, revealed the presence of cork cells'. The chemical characterization included summative chemical analysis, FTIR, GC-MS/FID for suberin composition and Py-GC-MS for lignin composition. <em>E. suberea</em> extractive-free barks <u>A</u> and <u>B</u> contained 29 % and 25 % suberin, 30 % and 37 % lignin, and 39 % and 38 % polysaccharides, respectively. The bark <u>A</u> exhibited a higher extractives content (49 %) than just 14 % for bark <u>B</u>. Dichloromethane extract from bark <u>A</u> was notably rich in alkanol ferulates. The main components of <em>E. suberea</em> <u>A</u> and <u>B</u> suberins were glycerol, with 15 % and 11 %, ω-hydroxyacids, with 45 % and 50 %, and α,ω-diacids, with 30 % and 17 %, representing a total of ca. 90 % and 78 % of <em>E. suberea</em> <u>A</u> and <u>B</u> suberins mass, respectively. Both suberins showed low contents of epoxides and <em>vic</em>-diols; also, both presented the particularity of having high contents of free acid groups, especially the suberin of bark <u>B</u>, suggesting a polyester structure with a smaller than usual degree of polymerization. <em>E. suberea</em> corky bark presents a lignin composition that differentiates from the cork G-lignins analyzed until now with a monomer composition exhibiting a GS character, with more G- (53–56 %) than S-units (34–37 %) with an S/G ratio between 0.64 and 0.66, for <u>A</u> and <u>B</u> lignins, respectively.</div></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"123 ","pages":"Article 105096"},"PeriodicalIF":2.0000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Discovering the corky bark of Eucalyptus suberea from anatomical and chemical perspectives – a case study\",\"authors\":\"Jorge Gominho , Ana Lourenço , Helena Patrício , Teresa Quilhó , António V. Marques\",\"doi\":\"10.1016/j.bse.2025.105096\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This paper presents the first scientific investigation of the corky bark of <em>Eucalyptus suberea</em> (cork mallee). The study involved two trees with diameters of 23 cm (A) and 47 cm (B), collected from a plantation in Portugal. Anatomical analysis, performed by scanning electron microscopy, revealed the presence of cork cells'. The chemical characterization included summative chemical analysis, FTIR, GC-MS/FID for suberin composition and Py-GC-MS for lignin composition. <em>E. suberea</em> extractive-free barks <u>A</u> and <u>B</u> contained 29 % and 25 % suberin, 30 % and 37 % lignin, and 39 % and 38 % polysaccharides, respectively. The bark <u>A</u> exhibited a higher extractives content (49 %) than just 14 % for bark <u>B</u>. Dichloromethane extract from bark <u>A</u> was notably rich in alkanol ferulates. The main components of <em>E. suberea</em> <u>A</u> and <u>B</u> suberins were glycerol, with 15 % and 11 %, ω-hydroxyacids, with 45 % and 50 %, and α,ω-diacids, with 30 % and 17 %, representing a total of ca. 90 % and 78 % of <em>E. suberea</em> <u>A</u> and <u>B</u> suberins mass, respectively. Both suberins showed low contents of epoxides and <em>vic</em>-diols; also, both presented the particularity of having high contents of free acid groups, especially the suberin of bark <u>B</u>, suggesting a polyester structure with a smaller than usual degree of polymerization. <em>E. suberea</em> corky bark presents a lignin composition that differentiates from the cork G-lignins analyzed until now with a monomer composition exhibiting a GS character, with more G- (53–56 %) than S-units (34–37 %) with an S/G ratio between 0.64 and 0.66, for <u>A</u> and <u>B</u> lignins, respectively.</div></div>\",\"PeriodicalId\":8799,\"journal\":{\"name\":\"Biochemical Systematics and Ecology\",\"volume\":\"123 \",\"pages\":\"Article 105096\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical Systematics and Ecology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0305197825001450\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Systematics and Ecology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0305197825001450","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Discovering the corky bark of Eucalyptus suberea from anatomical and chemical perspectives – a case study
This paper presents the first scientific investigation of the corky bark of Eucalyptus suberea (cork mallee). The study involved two trees with diameters of 23 cm (A) and 47 cm (B), collected from a plantation in Portugal. Anatomical analysis, performed by scanning electron microscopy, revealed the presence of cork cells'. The chemical characterization included summative chemical analysis, FTIR, GC-MS/FID for suberin composition and Py-GC-MS for lignin composition. E. suberea extractive-free barks A and B contained 29 % and 25 % suberin, 30 % and 37 % lignin, and 39 % and 38 % polysaccharides, respectively. The bark A exhibited a higher extractives content (49 %) than just 14 % for bark B. Dichloromethane extract from bark A was notably rich in alkanol ferulates. The main components of E. subereaA and B suberins were glycerol, with 15 % and 11 %, ω-hydroxyacids, with 45 % and 50 %, and α,ω-diacids, with 30 % and 17 %, representing a total of ca. 90 % and 78 % of E. subereaA and B suberins mass, respectively. Both suberins showed low contents of epoxides and vic-diols; also, both presented the particularity of having high contents of free acid groups, especially the suberin of bark B, suggesting a polyester structure with a smaller than usual degree of polymerization. E. suberea corky bark presents a lignin composition that differentiates from the cork G-lignins analyzed until now with a monomer composition exhibiting a GS character, with more G- (53–56 %) than S-units (34–37 %) with an S/G ratio between 0.64 and 0.66, for A and B lignins, respectively.
期刊介绍:
Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology).
In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.