一种评价生物膜伤口清洁的新型体外洗涤器模型。

IF 3.4 3区 医学 Q2 CELL BIOLOGY
Fergus Watson, Marcus J Swann, Jeanne Saint Bezard, Rui Chen, Alisha Oropallo, Steven L Percival
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引用次数: 0

摘要

慢性伤口是全球患者和医院的重大负担,所有伤口都表现出高微生物负荷和生物膜。伤口清洁对于清除外来污染物,受损组织和机会性病原体至关重要,从而改善愈合。本研究解决了标准化的体外临床前模型的需求,用于比较抗菌伤口清洁溶液与生物膜机械破坏的功效。开发了一种新的模型来模拟清洁实践,同时标准化施加的压力和施加在伤口床上的擦洗力。使用受伤的猪外植体,在暴露于不同的物理参数和清洁溶液之前,在表面形成微生物生物膜。结果表明,增加压力和洗涤时间对生物膜的去除都有积极的影响,表明微生物水平降低了bbbb1 log。该模型能够显示在相同条件下,生理盐水和基于抗菌剂的溶液(HClO)在清洁溶液中的显着差异,减少了>.5 log。使用荧光活性染色的共聚焦激光显微镜提供了使用纱布破坏生物膜的支持证据。该模型的适应性和多功能性有助于提供临床相关的体外证据,并通过不同清洁技术的标准化来有效比较伤口清洁剂对生物膜的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A Novel In Vitro Scrubber Model for Evaluating Wound Cleansing on Biofilms.

A Novel In Vitro Scrubber Model for Evaluating Wound Cleansing on Biofilms.

A Novel In Vitro Scrubber Model for Evaluating Wound Cleansing on Biofilms.

A Novel In Vitro Scrubber Model for Evaluating Wound Cleansing on Biofilms.

Chronic wounds are a significant burden on patients and hospitals globally, with all exhibiting high microbial loading and biofilm. Wound cleansing is critical for removing foreign contaminants, damaged tissue and opportunistic pathogens, allowing for improved healing. This study addresses the need for a standardised in vitro preclinical model for comparing the efficacy of antimicrobial wound cleansing solutions with mechanical disruption on biofilms. A novel model was developed to emulate cleaning practises whilst standardising pressure applied and scrubbing forces applied to the wound bed. Using wounded porcine explants, microbial biofilms were formed on the surface before exposure to varying physical parameters and cleaning solutions. The results showed that both increased pressure and scrubbing duration had a positive impact on biofilm removal, demonstrating > 1 log reduction in microbial levels. The model was able to show the significant difference in cleaning solutions between saline and an antimicrobial-based solution (HClO), > 4.5 log reduction, whilst under identical conditions. Confocal laser microscopy, using fluorescent viability stains, provided supporting evidence of biofilm disruption using gauze. The model's adaptability and versatility help to provide clinically relevant in vitro evidence and effective comparisons of wound cleansing agents on biofilms through the standardisation of different cleaning techniques.

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来源期刊
Wound Repair and Regeneration
Wound Repair and Regeneration 医学-皮肤病学
CiteScore
5.90
自引率
3.40%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Wound Repair and Regeneration provides extensive international coverage of cellular and molecular biology, connective tissue, and biological mediator studies in the field of tissue repair and regeneration and serves a diverse audience of surgeons, plastic surgeons, dermatologists, biochemists, cell biologists, and others. Wound Repair and Regeneration is the official journal of The Wound Healing Society, The European Tissue Repair Society, The Japanese Society for Wound Healing, and The Australian Wound Management Association.
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