{"title":"不同牙周病原菌感染DPSCs的细胞分化。","authors":"Tiansong Xu, Xiaofen Chai, Wen Zhang, Xueying Li, Yifei Zhang, Qian Zhang, Lin Yue, Xiaoying Zou, Feng Chen","doi":"10.1111/odi.70045","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>The aim of the present study was to investigate the effects of Capnocytophaga periodontitidis (C. periodontitidis) and Porphyromonas gingivalis (P. gingivalis) on dental pulp stem cells (DPSCs) subclusters.</p><p><strong>Materials and methods: </strong>DPSCs were collected from healthy donors and were infected with C. periodontitidis or P. gingivalis. High-throughput single-cell RNA sequencing of infected DPSCs was performed to analyze gene expression changes, focusing on differentiation, hypoxia, and cell cycle-related pathways.</p><p><strong>Results: </strong>Based on our single-cell resolution analysis, four major DPSC clusters were identified: classical-like, fibroblast-like, smooth muscle-like, and perivascular-like. C. periodontitidis infection induced differentiation into smooth muscle-like cells, while P. gingivalis promoted fibroblast (mineralized) lineages. P. gingivalis had a more significant effect on DPSCs differentiation. A specific subcluster responded uniquely to C. periodontitidis, with gene expression linked to B cell activation, muscle proliferation, and ROS pathways.</p><p><strong>Conclusion: </strong>DPSCs showed the homogeneity and heterogeneity when infected by these two pathogens. DPSCs differentiated into smooth muscle-related cells and mineralization-related cells when infected by C. periodontitidis and P. gingivalis, respectively. Our results will facilitate understanding and therapeutic strategies of primary periodontal disease with secondary endodontic involvement.</p>","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cell Differentiation of DPSCs Infected With Different Periodontal Pathogens.\",\"authors\":\"Tiansong Xu, Xiaofen Chai, Wen Zhang, Xueying Li, Yifei Zhang, Qian Zhang, Lin Yue, Xiaoying Zou, Feng Chen\",\"doi\":\"10.1111/odi.70045\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>The aim of the present study was to investigate the effects of Capnocytophaga periodontitidis (C. periodontitidis) and Porphyromonas gingivalis (P. gingivalis) on dental pulp stem cells (DPSCs) subclusters.</p><p><strong>Materials and methods: </strong>DPSCs were collected from healthy donors and were infected with C. periodontitidis or P. gingivalis. High-throughput single-cell RNA sequencing of infected DPSCs was performed to analyze gene expression changes, focusing on differentiation, hypoxia, and cell cycle-related pathways.</p><p><strong>Results: </strong>Based on our single-cell resolution analysis, four major DPSC clusters were identified: classical-like, fibroblast-like, smooth muscle-like, and perivascular-like. C. periodontitidis infection induced differentiation into smooth muscle-like cells, while P. gingivalis promoted fibroblast (mineralized) lineages. P. gingivalis had a more significant effect on DPSCs differentiation. A specific subcluster responded uniquely to C. periodontitidis, with gene expression linked to B cell activation, muscle proliferation, and ROS pathways.</p><p><strong>Conclusion: </strong>DPSCs showed the homogeneity and heterogeneity when infected by these two pathogens. DPSCs differentiated into smooth muscle-related cells and mineralization-related cells when infected by C. periodontitidis and P. gingivalis, respectively. Our results will facilitate understanding and therapeutic strategies of primary periodontal disease with secondary endodontic involvement.</p>\",\"PeriodicalId\":19615,\"journal\":{\"name\":\"Oral diseases\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-07-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Oral diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/odi.70045\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/odi.70045","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Cell Differentiation of DPSCs Infected With Different Periodontal Pathogens.
Aim: The aim of the present study was to investigate the effects of Capnocytophaga periodontitidis (C. periodontitidis) and Porphyromonas gingivalis (P. gingivalis) on dental pulp stem cells (DPSCs) subclusters.
Materials and methods: DPSCs were collected from healthy donors and were infected with C. periodontitidis or P. gingivalis. High-throughput single-cell RNA sequencing of infected DPSCs was performed to analyze gene expression changes, focusing on differentiation, hypoxia, and cell cycle-related pathways.
Results: Based on our single-cell resolution analysis, four major DPSC clusters were identified: classical-like, fibroblast-like, smooth muscle-like, and perivascular-like. C. periodontitidis infection induced differentiation into smooth muscle-like cells, while P. gingivalis promoted fibroblast (mineralized) lineages. P. gingivalis had a more significant effect on DPSCs differentiation. A specific subcluster responded uniquely to C. periodontitidis, with gene expression linked to B cell activation, muscle proliferation, and ROS pathways.
Conclusion: DPSCs showed the homogeneity and heterogeneity when infected by these two pathogens. DPSCs differentiated into smooth muscle-related cells and mineralization-related cells when infected by C. periodontitidis and P. gingivalis, respectively. Our results will facilitate understanding and therapeutic strategies of primary periodontal disease with secondary endodontic involvement.
期刊介绍：
Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.
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