使用智能DNA生物传感器的多重非侵入性生物标志物分析：乳腺癌转移诊断的最新趋势

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-07-29 DOI:10.1016/j.trac.2025.118395

Noshin Afshan, Yuna Guo, Jin Jiao

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引用次数: 0

摘要

DNA纳米技术通过有效的生物标志物识别、信号放大和简化分析，为早期诊断提供了强有力的方法。这篇综述探讨了多重生物标志物检测的潜力，重点介绍了多重靶点mirna、mrna、miRNA-mRNA分析、外泌体靶点、蛋白质和mirna -蛋白质相互作用的进展。多靶点方法与DNA纳米技术相结合，提高了诊断的敏感性、特异性和简便性，促进了及时的临床干预。本文对多种生物标志物检测的分析方法进行了分类，区分了非原位技术和原位技术。非原位检测是大规模筛查的理想选择，而原位检测可提供精确的空间数据。通过整合近期文献的见解，本综述旨在展示这些生物传感器在革命性诊断实践和改善患者预后方面的潜力。最终，它强调了多重生物标志物和DNA纳米技术在精准医学中的巨大潜力，反映了最近在早期检测、疾病监测和个性化治疗策略方面的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Multiplex non-invasive biomarkers profiling using smart DNA biosensors: recent trends in breast cancer metastasis diagnosis

DNA nanotechnology offers a powerful approach for early diagnosis by enabling efficient biomarker recognition, signal amplification, and streamlined analysis. This review explores the potential of multiplex biomarker detection, highlighting advancements in multiplex-target miRNAs, mRNAs, miRNA-mRNA analysis, exosomal targets, proteins, and miRNA-protein interactions. The multi-target approach, coupled with DNA nanotechnology, enhances sensitivity, specificity, and simplicity in diagnostics, facilitating timely clinical interventions. This review categorizes the analytical methods for multiplex biomarker detection, distinguishing between ex situ and in situ techniques. Ex situ detection is ideal for large-scale screenings, while in situ offers precise spatial data. By integrating insights from recent literature, this review aims to demonstrate the potential of these biosensors in revolutionizing diagnostic practices and improving patient outcomes. Ultimately, it underscores the remarkable potential of multiplex biomarkers and DNA nanotechnology in precision medicine, reflecting recent advancements in early detection, disease monitoring, and personalized therapeutic strategies.

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.