在高血糖大鼠模型中,湿暴露烧伤软膏通过激活SDF-1/CXCR4轴促进全层伤口愈合过程中的血管生成。

IF 3.4 3区 医学 Q2 CELL BIOLOGY
Naili Zhang, Li Fu, Jiayuan Xu, Chengxu Gu, Hongxing Li, Dong Wang, Tongshen Liu, Xueying Shi, Ning Xu, Jun Qiu, Lina Ma
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引用次数: 0

摘要

湿暴露烧伤软膏(MEBO)促进糖尿病足溃疡(DFUs)伤口愈合的机制尚不清楚。为了阐明这一机制,我们利用高血糖大鼠全层创面模型研究了基质细胞衍生因子-1 (SDF-1)在MEBO促进溃疡愈合和新生血管形成中的作用。在伤后第3、7、14天,MEBO组创面愈合率明显高于模型组和凡士林组(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Moist-Exposed Burn Ointment Promotes Angiogenesis During Full-Thickness Wound Healing by Activating the SDF-1/CXCR4 Axis in a Hyperglycaemic Rat Model.

The mechanism by which moist-exposed burn ointment (MEBO) promotes wound healing in diabetic foot ulcers (DFUs) remains unclear. To elucidate this mechanism, we investigated the role of stromal cell-derived factor-1 (SDF-1) in MEBO's ability to enhance ulcer healing and neovascularisation using a full-thickness wound model in hyperglycaemic rats. On post-wounding days 3, 7, and 14, the wound healing rates were significantly higher in the MEBO group compared to the model group and Vaseline group (p < 0.05). Additionally, the MEBO group exhibited increased epidermal layer thickness and enhanced collagen fibre deposition relative to the model group and Vaseline group (p < 0.05). Furthermore, the number of CD31-positive cells and microvascular density (MVD) were significantly elevated in the MEBO group compared to the model group and Vaseline group (p < 0.05). Flow cytometric analysis also demonstrated that the proportion of CD45-, CD34+, and VEGFR2+ cells in the wound area of the MEBO-treated group was significantly higher compared to the model group and Vaseline group. Expression levels of SDF-1, HIF-1α were also markedly higher in the MEBO group compared to the model group and Vaseline group (p < 0.05). Finally, a significant increase in double-positive CXCR4 and CD31 cells was observed exclusively in the MEBO group of hyperglycaemic rats (p < 0.05). These findings suggest that MEBO therapy promotes angiogenesis and accelerates wound healing through activation of the SDF-1/CXCR4 axis during wound healing in diabetics.

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来源期刊
Wound Repair and Regeneration
Wound Repair and Regeneration 医学-皮肤病学
CiteScore
5.90
自引率
3.40%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Wound Repair and Regeneration provides extensive international coverage of cellular and molecular biology, connective tissue, and biological mediator studies in the field of tissue repair and regeneration and serves a diverse audience of surgeons, plastic surgeons, dermatologists, biochemists, cell biologists, and others. Wound Repair and Regeneration is the official journal of The Wound Healing Society, The European Tissue Repair Society, The Japanese Society for Wound Healing, and The Australian Wound Management Association.
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